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pAxc

A cassette cosmid for construction of recombinant adenovirus

Catalog number RDB00918
Resource name pAxc
Alternative name pAdex1c
Clone info. A cassette cosmid for construction of recombinant adenovirus.
Comment Produce adenovirus by COS-TPC method.
Vector backbone pAxc (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42,464 bp
Selectable markers Amp^r
Depositor|Developer Saito, Izumu |
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            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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            Remarks Remember that you will be working with samples containing infectious virus.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
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            提供依頼書 [Word]
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            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB00918 pAxc Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pAxc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB00918).

            Reference section:

            Miyake, S., Makimura, M., Kanegae, Y., Harada, S., Sato, Y., Takamori, K., Tokuda, C., Saito, I., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP]
            Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP]
            original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

            2023.08.17

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