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pSVtsA58 ori (-)

Plasmid clone of SV40 temperature sensitive large T antigen cDNA

Catalog number RDB01126
Resource name pSVtsA58 ori (-)
Alternative name SV40 ori (-) DNA cDNA
Clone info. Plasmid clone of SV40 temperature sensitive large T antigen cDNA.
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4,361 bp
Selectable markers Amp^r
Gene/insert name SV40 temperature sensitive large T antigen cDNA
Depositor|Developer Ohno, Tadao |
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External Database
SV40 temperature sensitive large T antigen
            Reference sequence
              
             
            Remarks, protocol and/or map (png) RDB01126map_v2.png

            Distribution information

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            RDB01126 pSVtsA58 ori (-) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pSVtsA58 ori (-) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01126).

            Reference section:

            Ohno, T., Yamaguchi, N. , Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Human Genetics, 68, 209-210 (1984). PMID 6094336. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18365_B0Gap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: RDB08068_F1 (Pr0221)
            Region: AmpR pro
            Sequence file: RDB18365_B0Gaa.seq check
            >01126_18365_B0Ga_3_RDB08068_F1_F06_18_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTTCN CNNNNNNTTT NNATTACCCT TAGAAAGCGG TCTGTGAAAA
               61 ACCCCTACCC NATTTCCTTT TTGTTAAGTG ACCTAATTAA CAGGAGGACA CAGAGGGTGG
              121 ATGGGCAGCC TATGATTGGA ATGTCCTCTC AAGTAGAGGA GGTTAGGGTT TATGAGGACA
              181 CAGAGGAGCT TCCTGGGGAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA
              241 GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT
              301 CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG
              361 TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA
              421 ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG
              481 TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT
              541 TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT
              601 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG
              661 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
              721 TATGTATCCG CTCATGANAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA
              781 GANTATGAGT ATTCAACATT TCCGNNNCGC CCTTATTCCC TTTTTTTGCG GCATTTTNGC
              841 NTTCCTGNTT TTTGCTCACC CAGAAACGCN NGNNAAAGTA AAAGANGCNN AANNNCANTN
            901 NGGNNGCNCN NNNNGNNNAC NTCNNANNNN NNNNNN
            //
            Primer: pGL4pA_R (Pr0664)
            Region: SV40 pA, insert 3'
            Sequence file: RDB18365_B0Gab.seq check
            >01126_18365_B0Ga_3_pGL4pA_R_G06_21_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN CNNNANGCGN NTCCTGCATT AGGNAGCAGC CCAGTAGTAG
               61 GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA
              121 CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC
              181 GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC
              241 ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCC AGACATGATA
              301 AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT
              361 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT
              421 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
              481 AGCAAGTAAA ACCTCTACAA ATGTGATATG GCTGATCATG AACAGACTGT GAGGACTGTG
              541 AGGGCTGAGG GGCCTGAAAT GAGCCTTGAG ACTGTGAATC AATGCCTGTT TCATGCCCTG
              601 AGTCTTCCAT GTTCTTCTCC CCACCATCTT CATTTTTATC AGCATTTTCC TGGCTGTCTT
              661 CATCATCATT ATCACTGTTT CTTAGCCAAT CTAAAACTCC AGTTCCCATA GCCACATTAA
              721 ACTTCATTTT TTGATACACT GACAAACTAA ACTCTTTGTC CAATCTCTCT TTCCACTCCA
              781 CNATTCTGCT CTGAATACTT TGANCAAACT CAGCCACAGG TCTGTACNNA ATTAACATAA
              841 GAAGCAAAGC AATGCCACTT TNAATTATCC TCTTTTCTAA CAAAAACTCA CTGCNNTCCN
            901 NNNNATGCTT TAAATAATCT TNGGNNNNNA ANNCNANTTN NTTTANNANN NNNNNNNN
            //
            Primer: LTA_M_F (Pr0270)
            Region: insert mid (Ala438Val)
            Sequence file: RDB18365_B0Gac.seq check
            >01126_18365_B0Ga_3_LTA_M_F_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNCNNN NNGNTTNNNN NTCTGGCCTG NANNGTTTTA GGCACACTGT
               61 ACTCATTCAT GGNGACTATT CCAGGGGGAA ATATTTGAGT TCTTTTATTT AGGTGTTTCT
              121 TTTCTAAGTT TACCTTAACA CTGCCATCCA AATAATCCCT TAAATTGTCC AGGTTATTAA
              181 TTCCCTGACC TGAAGGCAAA TCTCTGGACT CCCCTCCAGT GCCCTTTACA TCCTCAAAAA
              241 CTACTAAAAA CTGGTCAATA GCTACTCCTA GCTCAAAGTT CAGCCTGTCC AAGGGCAAAT
              301 TAACATTTAA AGCTTTCCCC CCACATAATT CAAGCAAAAC AGCTGCTAAT GTAGTTTTAC
              361 CACTATCAAT TGGTCCTTTA AACAGCCAGT ATCTTTTTTT AGGAATGTTG TACACCATGC
              421 ATTTTAAAAA GTCATACACC ACTGAATCCA TTTTGGGCAA CAAACAGTGT AGCCAAGCAA
              481 CTCCAGCCAT CCATTCTTCT ATGTCAGCAG AGCCTGTAGA ACCAAACATT ATATCCATCC
              541 TATCCAAAAG ATCATTAAAT CTGTTTGTTA ACATTTGTTC TCTAGTTAGT TGTAGGCTAT
              601 CAACCCGCTT TTTAGCTAAA ACAGTATCAA CAGCCTGTTG GCATATGGTT TTTTGGTTTT
              661 TGCTGTCAGC AAATATAGCA GCATTTGCAT AATGCTTTTC ATGGTACTTA TAGTGGCTGG
              721 GCTGTTCTTT TTTAATACAT TTTTAAACAC ATTTCNAAAC TGTATTGAAA TTCCNAGNAC
              781 ATCNNNAGCA ATAACAACAC ATCATCACAT TTTNGTTTCC ATNNCATANT CNNNNACNNN
              841 CTTNNNNNNN ACTNNNTTAN NTTCCNNNGC TTCTNNNGGN NNNAAANCAT GCNNCNTNNN
            901 NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Ohno, T., Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Human Genetics, 68, 209-210 (1984). PMID 6094336. [link to RRC of NBRP]
            user_report Fukumitsu K., Establishment of a cell line derived from a mouse fetal liver that has the characteristic to promote the hepatic maturation of mouse embryonic stem cells by a coculture method. Tissue Eng. Part A., 15, 3847-3856 (2009). PMID 19558317. [link to RRC of NBRP]
            user_report Takagi Y., Different initial steps of apoptosis induced by two types of antineoplastic drugs. Biochem. Pharmacol., 76, 303-311 (2008). PMID 18573489. [link to RRC of NBRP]
            user_report Takasaki I., Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12. J. Cell. Biochem., 102, 1472-1485 (2007). PMID 17440958. [link to RRC of NBRP]
            user_report Tabuchi Y, Functional characterization of a conditionally immortalized mouse epididymis caput epithelial cell line MEPC5 using temperature-sensitive simian virus 40 large T-antigen. Biochem. Biophys. Res. Commun., 329, 812-823 (2005). PMID 15752729. [link to RRC of NBRP]
            user_report Takagi Y., Roles of MGMT and MLH1 proteins in alkylation-induced apoptosis and mutagenesis. DNA Repair (Amst)., 2, 1135-1146 (2003). PMID 13679151. [link to RRC of NBRP]

            2024.01.19

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