Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pco12 EGFR

Human EGF receptor (EGFR) cDNA

Catalog number RDB01276
Resource name pco12 EGFR
Alternative name Human EGF receptor cDNA
Clone info. Human EGF receptor (EGFR) cDNA
Vector backbone pCO12 (Plasmid)
Size of vector backbone 11.2 kb
Selectable markers Amp^r
Gene/insert name Human EGFR cDNA
Depositor|Developer Yamamoto, Tadashi |
Other clones in our bank

External Database
Human EGFR

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01276.gif
          Remarks, protocol and/or map (pdf) RDB01276.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01276 pco12 EGFR DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pco12 EGFR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01276).

          Reference section:

          Velu, T.J., Beguinot, L., Vass, W.C., Zhang, K., Pastan, I., Lowy, D.R. , Retroviruses expressing different levels of the normal epidermal growth factor receptor: biological properties and new bioassay. J. Cell Biochem., 39, 153-166 (1989). PMID 2565908.
          Velu, T.J., Beguinot, L., Vass, W.C., Willingham, M.C., Merlino, G.T., Pastan, I., Lowy, D.R., Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science, 238, 1408-1410 (1987). PMID 3500513.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Dnaconda's recommendation EXR0034e (English text)
          Featured content Dnaconda's recommendation EXR0034j (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18645_B0Lfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: lacIpro_R1 (Pr0762)
          Region: pBR322-BamHI-Ha MuSV (LTR)
          Sequence file: RDB18645_B0Lfa.seq check
          >01276_18645_B0Lf_1_lacIpro_R1_F02_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CGCTCNTGAG CCCGAAGTGG CGAGCCCGAT
             61 CTTCCCCATC GGTGATGTCN GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA
            121 TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCCGGATCTC CCTCTCCTCC AACCACCCTT
            181 GGAACGTCTT CCAGGGCTGC GGGGGAGAAG TCCGAGCTCG TCAGCTCCTT CTCTGGCCCG
            241 CCCAGATAGT CCCCAGATCT GAGCCTATTG ATCGATCGTT CACAGGACAA GACACAACAG
            301 ACAAGACAAA CGCCGGCCGG CTTGCCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG
            361 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCA CTAACGGGTA GTCAATCACT
            421 TAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
            481 TATTGGATAT ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC CCCGAGTGAG
            541 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC
            601 GAACTGATTG GTTAGTTTNA ATNANGNACA GGGTCATTTC AGGTCCNTGG GGNACCCTNN
            661 AAACNTCNGA NGNNTCTCTN NNNNTGCTGN NGNNTNNNNN GCNNCTGGGG NNCATCTNNN
          721 CNNGNNCNNN NNNNNGGNNN NNNNNNN
          //
          Primer: Amp_R (Pr0008)
          Region: pBR322 (AmpR pro)-HindIII-Ha MuSV
          Sequence file: RDB18645_B0Lfb.seq check
          >01276_18645_B0Lf_1_Amp_R_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNANNNGNN NCATTTATNA GGGTTATTGT CTCATGNAGC GGANACATAT
             61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
            121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
            181 CGAGGCCCTT TCGTCTTCAA GAATTAATTC TCATGTTTGA CAGCTTATCA TCGATAAGCT
            241 TTCCTCACCA AACGAGAGAC GGCCTCTACA ATCATCAAGA AGATACTGGA AGAAATCTTC
            301 CTCTTGGAAT GCCCAAGGTA ATCTGGTCAG ACAACGGCCC TACTTTCGTT GCCAAGGTAA
            361 GCCAGGGTGT GGCCAAGTAT TTAGAGGTCG ATTGAAAATT ACATTGTATT TACAGACCTC
            421 AAAGTTCAGG ACAGGTAGGG TAAATAAATA AAACTCTAAA AGAGACCCTG ACCAAATTAA
            481 CCATGGAGAC TGGCACAGAC TGGGTGACAC TCCTTCCCTC TTGCTCTCTT CAGAGCAAGA
            541 AATACCCCTT CCAGATTCNG CCTTACCCCC TTTGAGATCT TATATGGGGC CTCAGCCCCA
            601 CTGACTGTAT TANATGANGT TACTGAACCN ACATGTCATA GTGNCATAGT AATAATGANT
            661 TGTGTGNNNN GNNNAAANAN CTACANGNGN NACNGAAAGA AATCTGNTNN NNNNNNGNAN
          721 NNNNNNANNN CNNNGGNNNC CNNNNNNNNN N
          //
          Primer: ESFR-N_rev1 (Pr0576)
          Region: insert 5'(hEGFR 5')-SacII-Ha MuSV
          Sequence file: RDB18645_B0Lfc.seq check
          >01276_18645_B0Lf_1_ESFR-N_rev1_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC CTCCNNGNNG GTCTTTAAGA AGGAAAGANC ATAATTCCTC
             61 TGCACATAGG TAATTTCCAA ATTCCCAAGG ACCACCTCAC AGTTATTGAA CATCCTCTGG
            121 AGGCTGAGAA AATGATCTTC AAAAGTGCCC AACTGCGTGA GCTTGTTACT CGTGCCTTGG
            181 CAAACTTTCT TTTCCTCCAG AGCCCGACTC GCCGGGCAGA GCGCAGCCAG CAGCGCCAGG
            241 AGCGCTGCCC CGGCCGTCCC GGAGGGTCGC ATCGCTGCTC CCCGAAGAGC TCGCTCCGGC
            301 TCTCCCGATC AATACTGGAC GGAGTCAGGG GGCCGTGCGC GGTGGTTGTG GCGTTGGCGG
            361 CGAGGCGGGG ACTCGGGCGG ACGCCGACGA GGTGGCCTGT CGTCCGGTCT GGGCGGCGGC
            421 GGCCGCCGGG GCCATCGACT CTAGCGGATC CGCGGCGGGT GCGGCTCGGG CTGCGGGCAT
            481 AAGCACAGAT AAAACACTAT ATGTTTTTGT TTTGTTTCTC TTTCCTTTTT TTCAGAGCTG
            541 GGGACCGAAC CCAGGGCCTT GCGCTTGCTA GGCAAGAGCT CTATCACTGA NCTAAATCCC
            601 CAACCCCCAA AACACTATGT TTTAAAAATT AACTTTGGCT ATCNACNNAC ACACTGCCNC
            661 TAGAGCGGGG NCTACAAAAN TNNANTTTCN TANTCNCTAA NCNNNNANNN NNNNNANTAA
          721 ANCTNNTNNN NNNANNNAAN NNNAANNNNN TTNNNNNNNN NN
          //
          Primer: pCO12_R (Pr0240)
          Region: Ha MuSV-XhoI-insertt 3' (hEGFR 3')
          Sequence file: RDB18645_B0Lfd.seq check
          >01276_18645_B0Lf_1_pCO12_R_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNGCCGGGNN GGGCACAAAG GNCATGACTG ACCCTCGAGA
             61 CATGGGCCTG CAGGTCGATG GAAAGATAAA TTTCTGCTCA AAGGGACAAT ATTCTTGCTG
            121 GATGCGTTTC TGTAAATGCT TCACAGTTTG AAGACAAAGG AATGCAACTT CCCAAAATGT
            181 GCCCGAGGTG GAAGTACTTC CTGGCTAGTC GGTGTAAACG TTGCAAAACC AGTCTGTGGG
            241 TCTAAGAGCT AATGCGGGCA TGGCTGTTGG GATGGAGGAC CTGCTGTGGC TTGGTCCTGG
            301 GTATCGAAAG AGTCTGGATT TTTAGGGCTC ATACTATCCT CCGTGGTCAT GCTCCAATAA
            361 ATTCACTGCT TTGTGGCGCG ACCCTTAGGT ATTCTGCATT TTCAGCTGTG GAGCCCTTAA
            421 AGATGCCATT TGGCTTGGCT TCCTTGGGAA AGAAGTCCTG CTGGTAGTCA GGGTTGTCCA
            481 GGCTAATTTG GTGGCTGCCT TTCTGGGCCC AGTGGGCAGG GCTGTCGAAT GTGCTGTTGA
            541 CACAGGTGGG CTGGACAGTG TTGAGATACT CGGGGTTGCC CACTGCAGTG CTGTGGGGGT
            601 CCTGGTAGTG TGGGTCTCTG CTGGGCGCGG GGTTCANANG CTGANTGTGA TANACAGGAN
            661 TCTGCNCAGA NCCNGCGGGN CNTTTGGNAA CGGACTNNTT TANGNNTTNN NNNNCTGGGN
          721 NNNNANGNNG TCNNNNN
          //
          Primer: ESFR-C_F1 (Pr0938)
          Region: insert 3'-XhoI-Ha MuSV
          Sequence file: RDB18645_B0Lfe.seq check
          >01276_18645_B0Lf_1_ESFR-C_F1_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TCCNGNNNNN NNATTGTCCC TTTGANCAGA AATTTATCTT
             61 TCCNTCGACC TGCAGGCCCA TGTCTCGAGG GTCAGTCATG TCCTTTGTGC CCGTCCCGGC
            121 ACAGGCTCAG GACGAGGAGG TGCCGGATGC AGGGAGGTAG GTACTCCTTA AGGGAAAATC
            181 TAAGCCCAGA GAGACAGCCG GTAACAGCTA GGCTGCCTCT CTGCTCGCCT TTCTGTTTCA
            241 TAGACAAAGC TTGTGCTTAT ATTTACAGTG GCCTTTTTGT CCAAAAAAAG GCCTCCTGGT
            301 TTAGCTGTGT GACTAAATGC TATTTGTCCT CTTCAGTGGA CCTCTCTCCT CTAGATTCCC
            361 TTCTTGTTTT CTCACCATCC CACGTGAGAT GCTTGTTAGT AACTGTTACA GATCTTCTTT
            421 ACCACTGAGG AAAGACAGAA TCCTGCTAAA GGCCAGAAAG AATGTTCCAG ACATGGATGG
            481 AAGGCTCCTG ACTGTTGACT TCAATGCCCC TGAAGGTAGG GAGTGCTCCA GGTCTGCCCC
            541 CAGGCTCCGA GGGTGGGTCT CCTAGGGGCT AGAAAATGCC CCACNNATCT GGCTAAGATA
            601 AGAAAAAGAT ATAAAGAGAA AGTTACNGAA ACTTGAAGGG TAAAGCTAAG TCACTGAAAG
            661 AGNTATTATA AGTTACAGAN AAAATAANTT NANGCNNNNN NNCNNNNNNG NGNNNNAANN
          721 NNNNNNNN
          //
          Primer: HaMuSV-3pri_R2 (Pr 0946)
          Region: Ha MuSV (LTR)
          Sequence file: RDB18645_B0Lff.seq check
          >01276_18645_B0Lf_1_HaMuSV-3pri_R2_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNAGTAAAAC TCTTCGACGA ACAAAGCAAA CAGTTTCATG
             61 ATTTCAAACA CAGTCGTCGG TCTAAGATTT TAAACACAGT CGTCAGTCCA AATTCAAACA
            121 CGAAACAAAA GTCAAAAAGA CACTAACAGA CACAACACGT CCAGAAAACC ACAGTCAGGT
            181 CACAAAGAAG ACAAACAATT CCAACAGTCA AACAAGTAAC AAGCAGACGC GCCGCGCAGC
            241 TTCGGTACCA AACTGAAACC AAAAATTCAG ACGGAGTCAT CAAGGGTGCG GATCCCTCCG
            301 AAAACGGACG GAGGTGCCAC GGATCCGGAT CTCCCTCTCC TCCAACCACC CTTGGAACGT
            361 CTTCCAGGGC TGCGGGGGAG AAGTCCGAGC TCGTCAGCTC CTTCTCTGGC CCGCCCAGAT
            421 AGTCCCCAGA TCTGAGCCTA TTGATCGATC GTTCACAGGA CAAGACACAA CAGACAAGAC
            481 AAACGCCGGC CGGCTTGCCT CCCGGTGGTG GGTCGGTGGT CCCTGGGCAG GGGTCTCCCG
            541 ATCCCGGACG AGCCCCCAAA TGAAAGACCC CCACTAACGG GTAGTCAATC ACTTAGAGGA
            601 GACCCTCCCN AGGAACAGCG AGACCACAAG TCGGANGNNA CTGCNAGANG GTTTATTGNA
            661 NATACGGGTA CCCGGGCGAN TCNGTCNATC NNNNNANTGG NNNCCCNNNN GNGGGGNNGN
          721 GGNNNNNTTN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Velu, T.J., Retroviruses expressing different levels of the normal epidermal growth factor receptor: biological properties and new bioassay. J. Cell Biochem., 39, 153-166 (1989). PMID 2565908.
          original Velu, T.J., Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science, 238, 1408-1410 (1987). PMID 3500513.
          reference Clark, A.J., Synthesis of epidermal growth factor (EGF) receptor in vitro using SP6 RNA polymerase-transcribed template mRNA. Biochim. Biophys. Acta., 867, 244-251 (1986). PMID 3017432.
          user_report Su, J., Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens. Sci. Rep. 9 (1): 18189 (2019). PMID 31796769.
          user_report Fukuda, N., Rapid Evaluation of Tyrosine Kinase Activity of Membrane-Integrated Human Epidermal Growth Factor Receptor Using the Yeast G gamma Recruitment System. ACS Synth. Biol. 4 (4): 421-429 (2015). PMID 25006755.
          user_report Kojima, K., ESCRT-0 Protein Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (Hrs) Is Targeted to Endosomes Independently of Signal-transducing Adaptor Molecule (STAM) and the Complex Formation with STAM Promotes Its Endosomal Dissociation. J. Biol. Chem. 289 (48): 33296-33310 (2014). PMID 25296754.
          user_report Hiragun, T., Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines. Biosens. Bioelectron., 32 (1): 202-207 (2012). PMID 22204782.
          user_report Tanaka, K., Selection and growth regulation of genetically modified cells with hapten-specific antibody/receptor tyrosine kinase chimera. Biotechnol. Progr., 25, 1138-1145 (2009). PMID 19569211.
          user_report Dyson M.R., Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression BMC Biotechnol., 4, 1-18 (2004). PMID 15598350.
          user_report Niikura T., Insulin-like growth factor I (IGF-I) protects cells from apoptosis by Alzheimer's V642I mutant amyloid precursor protein through IGF-I receptor in an IGF-binding protein-sensitive manner. J. Neurosci., 21, 1902-1910 (2001). PMID 11245675.
          user_report Fujino T., Enhanced expression of heparin-binding EGF-like growth factor and its receptor in hypertrophied left ventricle of spontaneously hypertensive rats. Cardiovasc. Res., 38, 365-374 (1998). PMID 9709397.
          user_report Nakamura N., An epidermal growth factor receptor/Jak2 tyrosine kinase domain chimera induces tyrosine phosphorylation of Stat5 and transduces a growth signal in hematopoietic cells. J. Biol. Chem., 271, 19483-19488 (1996). PMID 8702638.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl