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Ax1w1 (#RDB01746)

A control virus with deletions of ElA, ElB and E3 genes

Clone info. A control virus with deletions of E1A, E1B and E3 genes and bearing no insert
Vector backbone pAxcw (Adenovirus)
Size of vector backbone -
Growth remarks 5 percent FCS is fine.
Gene/insert name AdV_5 -
Depositor|Developer Saito, Izumu |
 
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Miyake, S., Proc. Natl. Acad. Sci. USA, 93, 1320-1324(1996)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyake, S., Proc. Natl. Acad. Sci. USA, 93, 1320-1324(1996))。
備考 【追加提供依頼書】受入れ確認書(書式J)
組換え体提供にかかる書式が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB01746 Ax1w1 Under QC test. Please contact us. Virus, lysate of infected cell culture JPY 32,340 plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The Ax1w1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01746).

Reference section:

Miyake, S., Makimura, M., Kanegae, Y., Harada, S., Sato, Y., Takamori, K., Tokuda, C., Saito, I., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

References

Original, user report and related articles

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP]
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report Fukuda K., E1A, E1B double-restricted replicative adenovirus at low dose greatly augments tumor-specific suicide gene therapy for gallbladder cancer. Cancer Gene Ther., 16, 126-136 (2009). PMID 18818710. [link to RRC of NBRP]
user_report Wakayama M., E1A, E1B double-restricted adenovirus with RGD-fiber modification exhibits enhanced oncolysis for CAR-deficient biliary cancers. Clin. Cancer Res., 13, 3043-3050 (2007). PMID 17505007. [link to RRC of NBRP]
user_report Hayakawa Y., Thrombin-induced cell proliferation and platelet-derived growth factor-AB release from A172 human glioblastoma cells. J. Thromb. Haemost., 5, 2219-2226 (2007). PMID 17958740. [link to RRC of NBRP]
user_report Nonomura Y., Direct modulation of rheumatoid inflammatory mediator expression in retinoblastoma protein-dependent and -independent pathways by cyclin-dependent kinase 4/6. Arthritis Rheum., 54, 2074-2083 (2006). PMID 16802342. [link to RRC of NBRP]
user_report Hayakawa Y., Adenovirus-mediated expression of heparin cofactor II inhibits thrombin-induced cellular responses in fibroblasts and vascular smooth muscle cells. Thromb. Res., 116, 357-363 (2005). PMID 16038721. [link to RRC of NBRP]
user_report Fukuda K., E1A, E1B double-restricted adenovirus for oncolytic gene therapy of gallbladder cancer. Cancer Res., 63, 4434-4440 (2003). PMID 12907616. [link to RRC of NBRP]
user_report Nonomura Y., Gene transfer of a cell cycle modulator exerts anti-inflammatory effects in the treatment of arthritis. J. Immunol., 171, 4913-4919 (2003). PMID 14568973. [link to RRC of NBRP]
user_report Nonomura Y., Suppression of arthritis by forced expression of cyclin-dependent kinase inhibitor p21(Cip1) gene into the joints. Int. Immunol., 13, 723-731 (2001). PMID 11369699. [link to RRC of NBRP]
user_report Hirashima Y., Transfection of the plasma-type platelet-activating factor acetylhydrolase gene attenuates glutamate-induced apoptosis in cultured rat cortical neurons. Brain Res., 885, 128-132 (2000). PMID 11121539. [link to RRC of NBRP]