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Human HLA-C*07:02 cDNA

Human HLA-C*07:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02887
Resource name Human HLA-C*07:02 cDNA
Alternative name HLA-Cw*0702
Clone info. Human HLA-C*07:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0702 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*0702
          Reference sequence
            
           
          Sequence RDB02887z.seq
          Remarks, protocol and/or map (gif) RDB02887.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB02887 Human HLA-C*07:02 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-C*07:02 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02887).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18718_B1Bmp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB18718_B1Bma.seq check
          >02887_18718__B1Bm_2_CMV-Forward_A03_03_ABI24.ab1
              1 NNNNNNNNNN NTNNNNNNNN TGGGCGGTAG GCGTGTACGG TGGGAGGNNN NNNNAAGCAG
             61 AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA
            121 CTATAGGGAG ACCCAAGCTG GCTAGCGTTT AAACTTAAGC TTTTCTCCCC AGACGCCGAG
            181 ATGCGGGTCA TGGCGCCCCG AGCCCTCCTC CTGCTGCTCT CGGGAGGCCT GGCCCTGACC
            241 GAGACCTGGG CCTGCTCCCA CTCCATGAGG TATTTCGACA CCGCCGTGTC CCGGCCCGGC
            301 CGCGGAGAGC CCCGCTTCAT CTCAGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC
            361 GACAGCGACG CCGCGAGTCC GAGAGGGGAG CCGCGGGCGC CGTGGGTGGA GCAGGAGGGG
            421 CCGGAGTATT GGGACCGGGA GACACAGAAG TACAAGCGCC AGGCACAGGC TGACCGAGTG
            481 AGCCTGCGGA ACCTGCGCGG CTACTACAAC CAGAGCGAGG ACGGGTCTCA CACCCTCCAG
            541 ANGANGTCTG GCTGCGACCT GGGGCCCGAC GGGCGCCTCC TCCGCGGGTA TGANCAGTCC
            601 GCCTACGACG GCNAGGNNTA CATCNCCCTG AACNAGGANN NGCGCTCCTG GANCGNCNCG
            661 GANNNNGCGG NTNNNATNNC CCNNNGNNNG NNGNNNNNNC NNNGNGNNNN ANCNNCNNNN
          721 NNNNANNNGN NNGGNNNNNN NNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHpA,insert 3'
          Sequence file: RDB18718_B1Bmb.seq check
          >02887_18718__B1Bm_2_bGH_rev3_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGGG NNGGGCNNAC NANNGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCG TCTCAGGCTT
            121 TACAAGTGAT GAGAGACTCA TCAGAGCCCT GGGCACTGTT GCTGCACGCA GCCTGAGAGC
            181 AGCTCCCTCC TTTTCCACCT GAGCTCTTCC TCCTACACAT CATAGCGGTG ACCACAGCTC
            241 CAAGGACAGC TAGGACAACC AGGACAGCCA GGCCAGCAAC GATGCCCATG ATGGGGATGG
            301 TGGGCTGGGA AGATGGCTCC CAGCTCAGGG TGAGGGGCTC TTGCAGCCCC TCGTGCTGCA
            361 TATGGCACGT GTATCTCTGC TCTTGTCCAG AAGGCACCAC CACAGCTGCC CACTTCTGGA
            421 AGGTTCCATC TCCTGCTGGC CTGGTCTCCA CAAGCTCGGT GTCCTGGGTC TGGTCCTCCC
            481 CATCCCGCTG CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC CAGCACCTCN
            541 GGGTGGCCTC ATGGTCAGAG AGGGGGTGGT GGGTCACGTG TGTCTTTGGG GGNTCTGNGC
            601 GCTGCANCGT CTCCTTCCCG TTCNNNNGGT ATCTGCGGAN CCNCTCCACG CACGTGNCCT
            661 CCNGGTNNGN TCTCNNCTGC TNCNCCNCNN GGNNNGCCNN CNACNTNNNN NNGGNNGANN
          721 NNNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18718_B1Bmc.seq check
          >02887_18718__B1Bm_2_SV40pro_ori_F_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTC TGCCTCTGAG CTATTCNNGA AGTAGTGAGG
             61 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
            121 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
            181 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
            241 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
            301 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
            361 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
            421 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
            481 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
            541 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
            601 GGAAGCCGGN CTTGTCNATC NGGATGATCT GGNNNAAGAN NATCNNGGCT CNCGNCNNCC
            661 NAACTGTTCN NCNNGCTNNN GNGCGNANGN CCNANGNNNG NNNNNNTNNN GNNCCNNGNN
          721 NNNGCNNGNN NNNCNANNNN NNNGNNGNNA AANNNNNNNN NTTNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]
          user_report Komuro, H., Single-cell sequencing on CD8+ TILs revealed the nature of exhausted T cells recognizing neoantigen and cancer/testis antigen in non-small cell lung cancer. J. Immunother. Cancer 11 (8): e007180 (2023). PMID 37544663. [link to RRC of NBRP]

          2024.04.04

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