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Rat alpha-Antitrypsin RRRRSQ mutant

Expression vector of rat antitrypsin cDNA.

Catalog number RDB03411
Resource name Rat alpha-Antitrypsin RRRRSQ mutant
Clone info. Expression vector of rat antitrypsin cDNA. Expression was confirmed by depositor as follows: Immunoprecipitation and immunoblot.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Rat alpha1-antitrypsin cDNA
Depositor|Developer Tsuji, Akihiko |
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External Database
Rat alpha1-antitrypsin

          Reference sequence
            D00675
           
          Remarks, protocol and/or map (gif) RDB03411.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Tsuji, A. et al., Protein Eng. Des. Sel. 20 (4): 163-170 (2007)) are requested.
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          提供条件 利用者は、研究成果の公表にあたって文献 (Tsuji, A. et al., Protein Eng. Des. Sel. 20 (4): 163-170 (2007)) の引用を必要とする。
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          RDB03411 Rat alpha-Antitrypsin RRRRSQ mutant Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The Rat alpha-Antitrypsin RRRRSQ mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03411).

          Reference section:

          Tsuji, A., Kanie, H., Makise, H., Yuasa, K., Nagahama, M., Matsuda, Y., Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase. Protein Eng. Des. Sel. 20 (4): 163-170 (2007). PMID 17351018. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Tsuji, A., Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase. Protein Eng. Des. Sel. 20 (4): 163-170 (2007). PMID 17351018. [link to RRC of NBRP]

          2023.08.17

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