Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pUC18-HTK (with promoter)

Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene.

Catalog number RDB03436
Resource name pUC18-HTK (with promoter)
Clone info. Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene. It can be used to disrupt microbial genes using the kanamycin resistance genes.
Vector backbone pUC18 (Plasmid)
Gene/insert name Staphylococcus aureus kanamycin nucleotidyltransferase Genomic DNA
Reference of insert sequence AB121443 (DDBJ accession number)
Depositor|Developer Hoseki, Jun |
Other clones in our bank
External Database
Staphylococcus aureus kanamycin nucleotidyltransferase
            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB03436.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Hoseki, J. et al., J. Biochem. (Tokyo)., 126, 951-956, 1999)
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose [Word]
            Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hoseki, J. et al., J. Biochem. (Tokyo)., 126, 951-956, 1999)。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB03436 pUC18-HTK (with promoter) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pUC18-HTK (with promoter) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03436).

            Reference section:

            Hoseki, J., Yano, T., Koyama, Y., Kuramitsu, S., Kagamiyama, H., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            Tips

            Featured content

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13745_A7Kap1-4.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: AFR (Pr0324)
            Region: insert 5'( pro,HTK 5')
            Sequence file: RDB13745_A7Kac.seq check
            >03436_13745_A9EgF_AFR_D04_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNTGNN NNNGANNTCG AGCTCGGTAC CCGTTGACGG CGGANATGGT
               61 ACAATGAACA TGGAGACATG CAGGGTTTTC CTGCATTTTT CTTTGTGCAC TCATCAAGCG
              121 ATTTTCGGTA AAGCTAGGAG GAAATGATGA AACGACGAAT CAGCATCGCA ATCGACGGAC
              181 CCGCCGCGGC GGGGAAAAGC ACGGTCGCCA AACGGATCGC TGAGCGGCTT TCCTACGTAT
              241 ACATCGATAC CGGCGCCATG TATCGCGCGC TGACGTACCG GGCGCTTGCA TGCGGCGTCG
              301 ACTAGAATTC ATGAAAGGAC CAATAATAAT GACTAGAGAA GAAAGAATGA AGATTGTTCA
              361 TGAAATTAAG GAACGAATAT TGGATAAATA TGGGGATGAT GTTAAGGCAA TTGGTGTTTA
              421 TGGCTCTCTT GGTCGTCAGA CTGATGGGCC CTATTCGGAT ATTGAGATGA TGTGTGTTCT
              481 GTCAACAGAG GGAGTAGAGT TCAGCTATGA ATGGACAACC GGTGAGTGGA AGGCGGAAGT
              541 GAATTTTTAT AGCGAAGAGA TTCTACTAGA TTATGCATCT CGGGTGGAAC CGGATTGGCC
              601 GCTTACACAT GGTCGATTTT TCTCTATTTT GCCGATTTAT GATCCAGGTG GATACTTTGA
              661 GAAAGTGTAC CAAACTGCTA AATCGGTAGA AGCCCAAAAG TTCCACGATG CGATCTGTGC
              721 CCTTATCGTA NAAGAGCTGT TTGAATATGC AGGCAAATGG CGTAATATTC GTGTGCAAGG
              781 ACCGACAACA TTTCTACNAT CCTTGACTGT ACAGGTGGCA ATGNCANGTG CCATGTTGAT
              841 TGGTCTGCAT CATCGCATCT GTTATACNAC GAGCGCTTCN GTCTTAACTG AAGCANTTAA
              901 NCAACCANNT CTTCCTCCAG GTTATGTCCA ANTGTGCCAN CTCGNANTGT CTGGNCNNCT
              961 TTNCGANCNT GANAANNNTN NNNNNNNGCT AGANNNNTTC TGGNNNGGGG NNNCNNNNNN
            1021 NNNGNNNNNN NNNN
            //
            Primer: pUC-M13_-100 (Pr0310)
            Region: insert 3'(HTK 3')
            Sequence file: RDB13745_A7Kad.seq check
            >03436_13745_B1KoS_pUC-M13_-100_F02_17_ABI24.ab1
                1 NNNNNNNNNN CNNNNNNTTT CCCNGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT
               61 TGCATGTCTG CAGCGTAACC AACATGATTA ACAATTATTA GAGGTCATCG TTCAAAATGG
              121 TATGCGTTTT GACACATCCA CTATATATCC GTGTCGTTCC GCCCACTCCT GAACCCCATT
              181 CCAGAAATTC TCTAGCGATT CCAGAAGTTT CTCAGGGTCG GAAAGTTGAC CAGACATTAC
              241 GAGCTGGCAC AGTTGGACAT AACCTGGAGG AAGATCTGGT TGCTTAACTG CTTCAGTTAA
              301 GACCGAAGCG CTCGTCGTAT AACAGATGCG ATGATGCAGA CCAATCAACA TGGCACCTGC
              361 CATTGCCACC TGTACAGTCA AGGATGGTAG AAATGTTGTC GGTCCTTGCA CACGAATATT
              421 ACGCCATTTG CCTGCATATT CAAACAGCTC TTCTACGATA AGGGCACAGA TCGCATCGTG
              481 GAACTTTTGG GCTTCTACCG ATTTAGCAGT TTGGTACACT TTCTCAAAGT ATCCACCTGG
              541 ATCATAAATC GGCAAAATAG AGAAAAATCG ANCATGTGTA AGCGGCCAAT CCGGTTCCAC
              601 CCGANNNGCA TAATCTAGTA NAATCTCNTC GCTATNAAAN NTCACTTCNG CCTTNCACTC
              661 ANCGGNNGNC NNNTNNTANC TNNNNCNACT NNNCNGNNGN NNGNNNNCAN ATNNNCNNNN
            721 NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Hoseki, J., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290. [link to RRC of NBRP]
            user_report Jolly, S.M., Thermus thermophilus Argonaute Functions in the Completion of DNA Replication. Cell 182 (6): 1545-1559.e18. (2020). PMID 32846159. [link to RRC of NBRP]
            user_report Taniguchi, H., A Key Enzyme of the NAD+ Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures. J. Bacteriol. 199 (17). pii: e00359-17 (2017). PMID 28630126. [link to RRC of NBRP]
            user_report Ishida, K., Pseudouridine at position 55 in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus. Nucleic Acids Res., 39 (6): 2304-2318 (2011). PMID 21097467. [link to RRC of NBRP]
            user_report Shimada, A., A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways. Nucleic Acids Res., 38 (17), 5692-5705 (2010). PMID 20457749. [link to RRC of NBRP]

            2024.03.26

            HTML_filter_RDB_240326.pl