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pAxCAwtit2

Dual cassette vector to generate recombinant adenovirus containing CA promoter

Catalog number RDB05213
Resource name pAxCAwtit2
Clone info. A dual cassette for constructing recombinant adenovirus containing CAG promoter. Improved version of pAxCAwtit. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
Comment The pAxCAwtit2 cosmid is a derivative of pAxcwit2 (RDB no. 5212) and includes a CAG cassette [Niwa et al., Gene 108, 193-99, 1991], harboring a unique SwaI site as the cloning site for the gene of interest, at the E1 region. The pAxCAwtit2 cosmid, digested with Csp45I or PacI, can generate rAd upon transfection of HEK293 cells and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |
Other clones in our bank
External Database
AdV_5 -
            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB05213.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
            付加的提供条件：
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB05213 pAxCAwtit2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pAxCAwtit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05213).

            Reference section:

            Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., Saito, I., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
            Terashima, M., Kondo, S., Kanegae, Y., Saito, I., Exp. Med., 21, 931-936 (2003).

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13981_B1Lrp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CAG_R1 (Pr0584)
            Region: CAG pro,beta-globin pA
            Sequence file: RDB13981_B1Lra.seq check
            >05213_13981_B1Lr_1_CAG_R1_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNCT CNNGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA
               61 GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA
              121 GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT
              181 CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT
              241 CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA
              301 AGAATTGATT TATCGATTTA AATTATAAAC TAGTCTAGAA TCGATAATCA ATTCACTCCT
              361 CAGGTGCAGG CTGCCTATCA GAAGGTGGTG GCTGGTGTGG CCAATGCCCT GGCTCACAAA
              421 TACCACTGAG ATCTTTTTCC CTCTGCCAAA AATTATGGGG ACATCATGAA GCCCCTTGAG
              481 CATCTGACTT CTGGCTAATA AAGGAAATTT ATTTTCATTG CAATAGTGTG TTGGAATTTT
              541 TTGTGTCTCT CACTCGGAAG GACATATGGG AGGGCAAATC ATTTAAAACA TCAGAATGAG
              601 TATTTGGTTT AGAGTTTGGC AACATATGCC NNTATGCTGG CTGNNNTGAA CAAAGGNTGG
              661 CTATNAAGAN GNCNTCAGTA TATGNANNNN CCCCCNGCTG TNNNNTNCNN TANNNNNTAN
            721 AAAANNNNNN NNNN
            //
            Primer: Amp_R (Pr0008)
            Region: AmpR proITR.1,Ad5 psi
            Sequence file: RDB13981_B1Lrb.seq check
            >05213_13981_B1Lr_1_Amp_R_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTTTATCN GGGTTATTGT CTCNTGAGNN NNNNCATATT
               61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
              121 ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC
              181 GAGGCCCTTT CGTCTTCAAG AATTCCGGAT CGATCCGCAT GTTCGATTTC GATTTAATTA
              241 ATTCGAACAT CATCAATAAT ATACCTTATT TTGGATTGAA GCCAATATGA TAATGAGGGG
              301 GTGGAGTTTG TGACGTGGCG CGGGGCGTGG GAACGGGGCG GGTGACGTAG TAGTGTGGCG
              361 GAAGTGTGAT GTTGCAAGTG TGGCGGAACA CATGTAAGCG ACGGATGTGG CAAAAGTGAC
              421 GTTTTTGGTG TGCGCCGGTG TACACAGGAA GTGACAATTT TCGCGCGGTT TTAGGCGGAT
              481 GTTGTAGTAA ATTTGGGCGT AACCGAGTAA GATTTGGCCA TTTTCGCGGG AAAACTGAAT
              541 AAGAGGAAGT GAAATCTGAA TAATTTTGTG TTACTCATAG CGCGTAATAT TTGTCTAGGG
              601 CCGCGGGGNN TTTGANCGTT TACGTGGAGN CTCGCCCANG TGNTTTTCTC NNGTGTTTTC
              661 CGCGTNNCGG GNCAAANTTG GCGTTTTNNT ANTATAGTCN GCATCNNTTN ANCNNCNNNN
            721 NCNNNNNNGG NNNNNNGNNN NNNNNNN
            //
            Primer: AxIT_R (PrPr347)
            Region: ITR.2
            Sequence file: RDB13981_B1Lrc.seq check
            >05213_13981_B1Lr_1_AxIT_R_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNAAAC CNACAACTTC CTCAAATCGT CACTTCCGTT
               61 TTCCCACGTT ACGTCACTTC CCATTTTAAG AAAACTACAA TTCCCAACAC ATACAAGTTA
              121 CTCCGCCCTA AAACCTACGT CACCCGCCCC GTTCCCACGC CCCGCGCCAC GTCACAAACT
              181 CCACCCCCTC ATTATCATAT TGGCTTCAAT CCAAAATAAG GTATATTATT GATGATGTTC
              241 GAATTAATTA AATCGAAATC GAACATGCGG ATCCTCTAGA GTCAACAGCA GAAACATACA
              301 AGCTGTCAGC TTTGCACAAG GGCCCAACAC CCTGCTCATC AAGAAGCACT GTGGTTGCTG
              361 TGTTAGTAAT GTGCAAAACA GGAGGCACAT TTTCCCCACC TGTGTAGGTT CCAAAATATC
              421 TAGTGTTTTC ATTTTTACTT GGATCAGGAA CCCAGCACTC CACTGGATAA GCATTATCCT
              481 TATCCAAAAC AGCCTTGTGG TCAGTGTTCA TCTGCTGACT GTCGACCTGC AGGCATGCAA
              541 GCTTTAATGC GGTAGTTTAT CACAGTTAAA TTGCTAACGC AGTCAGGCAC CGTGTATTAA
              601 ATCTAACAAT GCGCTCATCG TCATCCTCGG CACCGTCACC CTGNANGCTG TANGCATANG
              661 CTNNNNTATG CCNGNACTGN CGGGCCTCTT GNGGGANATC GTCCATTNCN NANNNCATCN
            721 NNNGNCACNN NNNNNNGNNN CNANCGNNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
            original Terashima, M., Exp. Med., 21, 931-936 (2003).
            user_report Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551. [link to RRC of NBRP]

            2023.06.09

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