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pKM2L-phMK

Promoter Bank clone, Human midkine (MK) promoter

Catalog number RDB05514
Resource name pKM2L-phMK
Alternative name p1928-13F
Clone info. PCR amplified human midkine (MK) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 46379423 to 46381740(NC_000011.10); relatively -1645 to +673, where +1 corresponds to 1 nt of NM_001012334.2; relatively -2187 to +131, where +1 corresponds to 1 nt of NM_002391.4.
Comment PCR cloning forward primer, H7PB0056F: 5' gatcaggggacgggatggggtac 3'; reverse primer, 1928 R2: 5' gcttcgctccctcccgcgccgggt 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human midkine (neurite growth-promoting factor 2) genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human midkine (neurite growth-promoting factor 2)
          Reference sequence
            D10604, AC116021
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
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          RDB05514 pKM2L-phMK Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pKM2L-phMK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05514).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          user_report Maldonado AR., Molecular engineering and validation of an oncolytic herpes simplex virus type 1 transcriptionally targeted to midkine-positive tumors. J. Gene. Med., 12, 613-623 (2010). PMID 20603890.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl