Expression vector of human MAZ in E. coli
Clone info. | Expression vector of human MAZ in E. coli. |
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Comment | This clone contains a cDNA fragment corresponding to the 174 to 1607nt of NM_002383.4. Known differences (at least): 15nt (ggcggcagcggcagc) deletion at 1493 to 1507nt. |
Vector backbone | pGEX-4T-2 (Plasmid) |
Size of vector backbone | 4.97 kb |
Selectable markers | Amp^r |
Gene/insert name | Human MYC-associated zinc finger protein (purine-binding transcription factor) cDNA |
Depositor|Developer | Yokoyama, Kazunari | Song, Jun | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).). Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。共著とする。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem. Biophys. Res. Commun., 226, 801-809 (1996).)。本リソースを使用して商業利用する場合は、寄託者と改めて契約する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB05701 | pGEX-hMAZ | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pGEX-hMAZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05701). |
Reference section:
Song, J., Ugai, H., Ogawa, K., Wang, Y., Sarai, A., Obata, Y., Kanazawa, I., Sun, K., Itakura, K., Yokoyama, K.K., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18977_B1Dkp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pGEX-F2 (Pr0403) Region: GST,thronbin site,insert 5' Sequence file: RDB18977_B1Dka.seq |
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>05701_18977_B1Dk_3_pGEX-F2_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTATTGNA GCTATCCCAC AAATTNNATA AGTACTTGAA 61 ATCCAGCAAG TATATAGCAT GGCCTTTGCA GGGCTGGCAA GCCACGTTTG GTGGTGGCGA 121 CCATCCTCCA AAATCGGATC TGGTTCCGCG TGGATCCCCA GGAATTCTGC AGTCGACGGT 181 ACCGGGCCCC GCTGCGGCCG AGGCCATGTT CCCGGTGTTT CCTTGCACGC TGCTGGCCCC 241 CCCCTTCCCC GTGCTGGGCC TGGACTCCCG GGGGGTGGGC GGCCTCATGA ACTCCTTCCC 301 GCCACCTCAG GGTCACGCCC AGAACCCCCT GCAGGTCGGG GCTGAGCTCC AGTCCCGCTT 361 CTTTGCCTCC CAGGGCTGCG CCCAGAGTCC ATTCCAGGCC GCGCCGGCGC CCCCGCCCAC 421 GCCCCAGGCC CCGGCGGCCG AGCCCCTCCA GGTGGACTTG CTCCCGGTGC TCGCCGCCGC 481 CCAGGAGTCC GCCGCGGCTG CTGCGGCCGC TGCCGCCGCT GCTGCCGCCG TCGCTGCCGC 541 GCCCCCGGCC CCTGCCGCCG CCTCTACGGT GGACACAGCG GCCCTGAAGC AGCCTCCGGC 601 GCCCCCTCCG CCACCCCCGC CAGTGTCGGC GCCCGCGGCC GANGCCGCGC CCCCCGCCTC 661 CGCCGCCACT ATCGCCGCGG CGGCGGNCNN CGCNNNCNNA NCCCCNNNCT NNNNNNNNNN // |
Primer: pJB8-Forward (Pr0084) Region: insert 3' Sequence file: RDB18977_B1Dkb.seq |
>05701_18977_B1Dk_3_pJB8-Forward_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG TNNTGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC 61 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG 121 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGATCGTCAG 181 TCAGTCACGA TGCGGCCGCT CGAGTCGACC CGGGAATTCC CCTAAGAAAA CATTTCTTCC 241 TCCTTTCCTT CTGGAGGCTC CTTGGTTGGT AGGGAAATAG GAGTTGGTGG GAAAAGAGGG 301 GGGAGAGGAG CTTGTACCAA GGGACTCTAC TCCATTCTCC CCTCTCCCCC GCAACCAACT 361 TGGAGCTCAC CAGGGTTGGG AGGGAAGTGG CTGAGAGCTC ACAGGCACCC CCTCCGCCCC 421 CGAGAGGGAG CCCACAGCTG TGGGAGGGGC TGCTACTGCT GCCGCTGCTG CCGCTGCCGC 481 CGCCATTGGA CAAACCTCAC CAGTACCTTT GTTGCAGAGC TCACAGACAT GGTGAGGACC 541 CTGGCTGTGC ACCTTCATGT GGTCCGAAAT ATAAGCCGAG CTCAGCATCT TGCCACACAC 601 GTGACATGGC ACTTTCTCCT CGTGTCGNAC TGTGTGCGCC CGCAGCCGAT CCTTCNTGGC 661 GAANGCTGCC TCACATTTCT CACATTTGAA NGNCCNNTCN GNNNAGNGNA CNTNNNCNNN 721 NNNNNNN // |
Primer: lacIpro-B_F (Pr0724) Region: lacI pro,lacI Sequence file: RDB18977_B1Dkc.seq |
>05701_18977_B1Dk_3_laclpro-B_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTCNGTGNN CGANGAANCG GAAGANCGCC TGNTGCGGTA 61 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA AATTCCGACA CCATCGAATG 121 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT 181 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC 241 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA 301 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA 361 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT 421 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT 481 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT 541 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC 601 CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAN CAGACACCCA TCNACAGTAT 661 TANTTTCTNC CNTGNNGNNN NNCNCNNCTG GNNNNNNNNN N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515. [link to RRC of NBRP] |
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user_report | Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res., 41(7):4049-4064 (2013). PMID 23471001. [link to RRC of NBRP] |
user_report | Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711. [link to RRC of NBRP] |
user_report | Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603. [link to RRC of NBRP] |
user_report | Wang H.Y., Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome. BMC Mol. Biol., 8:89 (2007). PMID 17927842. [link to RRC of NBRP] |
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