RDB05727 - pZErO-2.1 mG9a-S

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pZErO-2.1 mG9a-S

Plasmid clone of mouse histone methyltransferase, G9a

Catalog number	RDB05727
Resource name	pZErO-2.1 mG9a-S
Clone info.	Plasmid clone of mouse histone methyltransferase, G9a.
Vector backbone	pZErO-2.1 (Plasmid)
Size of vector backbone	3.3 kb
Selectable markers	Kan^r
Gene/insert name	Mouse G9a cDNA
Reference of insert sequence	AB077209 (DDBJ accession number)
Depositor\|Developer	Shinkai, Yoichi \| Tachibana, Makoto \|

Other clones in our bank

mouse G9a

External Database
Mouse G9a

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes Dev., 16, 1779-1791, 2002).

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes Dev., 16, 1779-1791, 2002).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Genes Dev., 16, 1779-1791, 2002)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05727	pZErO-2.1 mG9a-S	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pZErO-2.1 mG9a-S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05727).

Reference section:

Tachibana, M., Sugimoto, K., Nozaki, M., Ueda, J., Ohta, T., Ohki, M., Fukuda, M., Takeda, N., Niida, H., Kato, H., Shinkai, Y., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev., 16, 1779-1791 (2002). PMID 12130538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15802_A8Bgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB15802_A8Bga.seq
>05727_15802_A8Bg_M13_-40_E02_14_ABI24.ab1 1 AGGGGCGGGG TAAATGGGAA TACGGACTTC GAATAGGGCG AATTGGGCCC TCTAGATGCA 61 TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CGCGGCCGCG TCGACGCGAT 121 GGCGGCGGCG GCGGGAGCTG CTGCGGCGGC GGCCGCCGAG GGGGAGGCCC CCGCTGAGAT 181 GGGGGCGCTG CTGCTGGAGA AGGAGCCCCG AGGAGCCGCC GAGAGAGTTC ATAGCTCTTT 241 GGGGGACACC CCTCAGAGTG AGGAGACCCT TCCCAAGGCC AACCCCGACT CCTTGGAGCC 301 TGCCGGCCCC TCCTCTCCGG CCTCTGTCAC TGTCACCGTC GGCGATGAGG GGGCTGACAC 361 CCCTGTCGGG GCCGCATCAC TCATCGGGGA CGAACCCGAG AGCCTGGAGG GAGATGGGGG 421 TCGCATCGTG CTGGGCCATG CCACAAAGTC GTTCCCCTCT TCCCCCAGCA AGGGGGGTGC 481 CTGTCCCAGT CGGGCCAAAA TGTCAATGAC AGGGGCAGGA AAGTCGCCCC CCTCGGTCCA 541 GAGTTTGGCC ATGAGGCTGT TGAGCATGCC CGGGGCCCAG GGAGCTGCAA CTGCTGGGCC 601 TGAACCCTCT CCGGCAACAA CTGCCGCCCA GGAGGGGCAG CCCAAAGTGC ACCGAGCCCG 661 GAAAACCATG TCCAAACCTA GCAACGGACA GCCTCCAATC CCTGAGAAGC GGCCCCCTGA 721 AGTCCAGCAT TTCCGCATGA GTGATGACAT GCATCTGGGG AAGGTGACTT CAGATGTGGC 781 CAAAAGGAGG AAGCTGAACT CTGGTAGCCT GTCCGAGGAC TTGGGCTCTG CCGGGGGGCT 841 CAGGAGATAT AATCCTGGAG AAGGGAGAGC CCAGGCCCCT GGAGGAGTGG GAGACGGTGG 901 TGGGCGATGA CTTCAGCCTG TACTATGATG CGTACTCTGT GGATGAGCGG GTGGACTCTG 961 ACAGCAAGTC TGAAGTCGAA GCTCTAGCTG AACAGTTGAG TGAGGAGGAG GAGGAGGAAG 1021 AGGAGGAAGA GAGGAAGAGG AAGGGAAGGA GGA //
Primer: Reverse2 Sequence file: RDB15802_A8Bgb.seq
>05727_15802_A8Bg_Reverse2_F02_17_ABI24.ab1 1 ATTAAATGGA ATTACGGCCA GGCTATTTAG GTGACACTAT AGAATACTCA AGCTATGCAT 61 CAAGCTTGGT ACCGAGCTCG GATCCACTAG TAACGGCCGC CAGTGTGCTG GAATTCGCGG 121 CCGCGTCGAC TTTTTTTTTT TTTACCAAAC CCAACATTTA TTGAGAACAC AGAAAATCAG 181 CTGGCAGAGG CCCAACTTCA ATCAGCTAAC TGAGGCCAGG CTCAGTGGGG AAGGCCAGCC 241 CTGAAGTCAC TTGCACTCCC AGTGAGAAAC CAGCTCTGGG AGAGGGGCCA TCGACCTTCC 301 AGGCCTTACT GGGGTCTCTG ATGGAGAAAT GTCTGAAGGG CAGTGCCCGG CCTGGATTTT 361 AAGAGTCCTC AGGTGTTGAT GGGGGGCAGG GAGCTGAGGT CAGGGAGCAG CTCCGGGTGG 421 GGGTCCAGCC GGGCCAGGCG GCTCTGCTCC AGGGCGATGG CCTCCGCTGA ATGCTTGCAC 481 TTCTCAGAGC CACACTGGCA GGTGAAATAC TTGCTCTTGA TGTCCCAGAA TCGGTCACCG 541 TAGTCAAAGC CCAGCTCCTC CCCAGTCCGG ATGTCCCTGG AGCTGAAGAA GGCAATGCGT 601 GGGAACCGTA GATCTTGGTG CAGCATGAAA ACCCGGACAG GGATGATGTT GGGGTCACAC 661 AGGTGGTTAA TGAATCGGCT GATGTTGCCA TAGTAACGGG CATCAATGCA GTAAACCTCG 721 CCATCCTTGT TATCTAAATC GAAGAGGTAA GAATCATCCT CTCTCACATC AGCCTCGGCA 781 TCAGAGATCA GCTCTCCTAC ATACTCGCAG ATGAACGTGC CCTGGGGGAT GGTCTGCAAG 841 GCTCGGACCC CCCAGCCCAT CTTGGCAGTC CGGTAGAGCT GCAGCCGTAC CTTGATGCCG 901 CTCTGCACCA CGCGGTTCTT GCAGCTTCTC CAGCAGGAGC ATGCCTGGGT TACACTCAAA 961 GATCAGGGGG GGCTCGATCT TGTTTAACTC CTGGAGCAGC CCGCCCGTCC CTTGTCATAC 1021 CAGCATCGGA TACTGAGCTG ACCACATAGG CATTGGGAGC TGGGAGCAGT CATCCACCAC 1081 CACGGTGCAG TGCTTGCAGA TGGGGTTGAT TGTTTGCGTC GGAATTGTTC ATTGGTCGAA 1141 TTGTTCTCGC AGTTCTCAGA GAATGTACTG AGTCCTCAGG GCACCGTGCG AGCCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tachibana, M., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev., 16, 1779-1791 (2002). PMID 12130538. [link to RRC of NBRP]

2023.08.18

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