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pKM2L-phP21

Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter

Catalog number RDB05893
Resource name pKM2L-phP21
Alternative name p1978-6F
Clone info. PCR amplified human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 36676033 to 36678769(NC_000006.12); relatively -2646 to +91, where +1 corresponds to 1 nt of NM_000389.4.
Comment PCR cloning, forward primer, 1978 F2: 5' aaaagccagggctgcctctgctcaa 3'; reverse primer, 1978 R2: 5' atccgcgcccagctccggctccaca 3' Entire sequence of promoter region has not been confirmed. (reference: J. Immunol., 172, 5006-5015, 2004, PMID:15067082)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1
          Reference sequence
            Z85996
           
          Sequence RDB05893z.seq

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05893 pKM2L-phP21 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pKM2L-phP21 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05893).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05893_06Irp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40 (Pr0410)
          Region: insert5'
          Sequence file: RDB05893_06Ira.seq check
          >05893_05893_06Ir_M13_-40_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNGCCTGCN GGTCNTTTAA ATAGGGNNAA CAGGGTAATG
             61 ATTAAGATCT CGAGCTCTAA GCTTCACATA TGCATGCACT AGTGGCAAAA GCCAGGGCTG
            121 CCTCTGCTCA ATAATGTTCT ATCTTTGTTC CGCCTCTTCT CTGGGGTCTC ACTTCTTGGG
            181 AGCCTGTGTG AAGGTGAATT CCTCTGAAAG CTGACTGCCC CTATTTGGGA CTCCCCAGTC
            241 TCTTTCTGAG AAATGGTGAC ATTGTTCCCA GCACTTCCTC TCCCTTCCTA GGCAGCTTCT
            301 GCAGCCACCA CTGAGCCTTC CTCACATCCT CCTTCTTCAG GCTTGGGCTT TCCACCTTTC
            361 ACCATTCCCC TACCCCATGC TGCTCCACCG CACTCTGGGG AGGGGGCTGG ACTGGGCACT
            421 CTTGTCCCCC AGGCTGAGCC TCCCTCCATC CCTATGCTGC CTGCTTCCCA GGAACATGCT
            481 TGGGCAGCAG GCTGTGGCTC TGATTGGCTT TCTGGCCGTC AGGAACATGT CCCAACATGT
            541 TGAGCTCTGG CATAGAAGAG GCTGGTGGCT ATTTTGTCCT TGGGCTGCCT GTTTTCAGGT
            601 GAGGAAGGGG ATGGTAGGAG ACAGGAGACC TCTAAAGACC CCAGGTAAAC CTTAGCCTGT
            661 TACTCTGAAC AGGGTATGTG ATCTGCCAGC AGATCCTTGC GACAGGGCTG GGATCTGATG
            721 CATGTGTGCT TGTGTGAGTG TGTGCTGGGA GTCAGATTCT GTGTGTGACT TTTAACAGCC
            781 TGCTCCCTTG CCTTTTTCAG GGCAGAAGTC CTCCCTTAGA GTGTGTCTGG NTACACATTC
            841 NAGTGCATGG TTGCNAACTT TTTTTTTTAA NGCACTGNAA TAGNACTANA CACTTAGTAN
            901 GNACTTAANA AATATTGAAT GTCGTGGTGG TGGTGAGCTA NAAGTTATAA AAAAAANTTC
            961 TTTTNCNNAA AACNANCNNC AAAAAGAANT NATTNNNTNN NTGAANNNNA NNANNNNAAA
          1021 AAATTTAAAN NNANNNNNNN NNN
          //
          Primer: hRluc_N_rev (Pr0567)
          Region: hRluc 5',insert 3'
          Sequence file: RDB05893_06Irb.seq check
          >05893_05893_06Ir__hRluc_N_rev_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNTT TGCTTGNNGC GAGCCCNCNN CTGNNNGCCC AGTGATCNNN
             61 GCGTTTGCGT TGCTCGGGGT CGTACACCTT GGAAGCCATG GTGGCTAGCC TATAGTGAGT
            121 CGTATTAAGT ACTCTAGCCT TAAGAGCTGT AATTGAACTG GGAGTGGACA CCTGTGGAGA
            181 GAAAGGCAAA GTGGATGTCA GTAAGACCAA TAGGTGCCTA TCAGAAACGC AAGAGTCTTC
            241 TCTGTCTCGA CAAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC
            301 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA
            361 CAGGCATCCG CGCCCAGCTC CGGCTCCACA AGGAACTGAC TTCGGCAGCT GCTCACACCT
            421 CAGCTGGCGC AGCTCAGCGC GGCCCTGATA TACAACCGCC CCGCCCGGGC CCGCCTCAAG
            481 GAGGCGGGAC CCGCGCTCGG CCCACCGCGC CGCCCGGGAC CGCCCTCCCC GGGGCCCAGG
            541 CTCCTGGCTG CCCAGCGCCG AGCCAGCTGA GCCTGGCCGA GTTCCAGCAG GCCAGCCGGT
            601 CCCGGAACCT CGCGTGCTGC AGGAGGGCAC TTCCCTCCTC CCCCAGTCCC TCGCCTGCGT
            661 TGGTGCGCTG GACACATTTC CCCACGAAGT GAGCCACAAA TCTGGCTTTT TTTACTTGGA
            721 GAATGAGTTG GCACTCTCCA GGAGGACACA GCACTGTTAG AATGAGCCCC CTTTCTGGCT
            781 CACCGCTGNN CCACTCTGGC AGGCAAGGAT TTACCCAATG CAGCTGAAAA AGATCAGGAN
            841 GANGACATTA ATACATAAAA ATTCATAAAT TATAAAAACG ATGCACCTCT CTGCANNTTC
            901 NANAAAAANC CCCNCNATAT CNCCTCTATT CCCACTGATN CCTCACTAGN TCACCTCTCC
            961 NANAANCNNC TGNANCANCT ANACNCCCCA ACAAANCNNC NTNGANNNCN NAANGATTTT
          1021 TCAGNCCNNN TNNNTTCNCA NNNNNNNAAN NNGNNNNCCN NNNNNNNTNN NNNNNNNN
          //
          Primer: pAxCALNLF1 (Pr0166)
          Region: hRluc mid
          Sequence file: RDB05893_06Irc.seq check
          >05893_05893_06Ir_pAxCALNLF1_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG TCTGNTCGNN NCGGCCGCTC TAGAATTACT GCTCGTTCTT
             61 CNGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG
            121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC
            181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC
            241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC
            301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC
            361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT
            421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT
            481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC
            541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG
            601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG
            661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC
            721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC
            781 GATGTGAGGC ACGACGTGCC TCCNCAGGTA GCTGGAGGCA GCGTNACCAT GCAGAAAAAT
            841 CACGGCGTTC TCGGCGTGCT TNNCGNAATC ATAGNNNNTG ANGAANGANT CCAGCACGTT
            901 CATTTGCTTG CAGCGAGCCC NCCACTGANG NCCAGTGATC ATGCGTTTGN GTNGCTCGGG
            961 GNCNTANACN TNGNAAGNNN NGGTGNNNNN NNNATANNGA GTCGTATTNN NNNNNNTANN
          1021 CNNTNNNNNN CTGNNATTGN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Ishigro, N., ASPL-TFE3 Oncoprotein Regulates Cell Cycle Progression and Induces Cellular Senescence by Up-Regulating p21. Neoplasia 18 (10): 626-635 (2016). PMID 27673450. [link to RRC of NBRP]
          user_report Suzuki, H., TATA-binding protein (TBP)-like protein is required for p53-dependent transcriptional activation of upstream promoter of p21Waf1/Cip1 gene. J. Biol. Chem., 287 (24): 19792-19803 (2012). PMID 22511763. [link to RRC of NBRP]
          user_report Matsumura, K., Involvement of the estrogen receptor beta in genistein-induced expression of p21(waf1/cip1) in PC-3 prostate cancer cells. Anticancer Res., 28 (2A): 709-714 (2008). PMID 18507011. [link to RRC of NBRP]

          2024.03.26

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