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S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter
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External Database Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
| Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。 |
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| 提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ] |
| 備考 | このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB06166 | pDUAL2-FFH1c | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Sequence | RDB06166z.seq |
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Original reference
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB13299_A5Df.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: nmt1Fw Sequence file: RDB13299_A5Dfa.seq
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>06166_13299_A5Df_nmt1Fw_G02_20.ab1 1 TATTTATTTA ATCAGGAAAC GTAACTCTCG GCTACTGGAT GGTTCAGTCA CCCAACGATT 61 ACTGGGGAGA GAAAACAGGG CAAAAGCAAA GCTTAAAGGA ATCCGATTGT CATTCGGCAA 121 TGTGCAGCGA AACTAAAAAC CGGATAATGG ACCTGTTAAT CGAAACATTG AAGATATATA 181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC 241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG 301 GATATCAACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA 361 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT 421 CCAGTCACTA TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG 481 TATAATGTGT GGATTTTGAG TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA 541 ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA 601 CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT 661 ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT 721 CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT 781 GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA 841 ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT 901 TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG 961 AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG 1021 GCCATATGGG ACACTTCTTC GCCCCGTTTT CACATGGCAA ATATATACGC AGCCGACAGT 1081 GCTGATGGCG CTGCGAATCA GGTTCATCAT GGCGTTGTGA TGGCCTTCCA ATGTCGGCGA 1141 GAATGCTTAA TGGCAT // |
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Primer: ADH_rev Sequence file: RDB13299_A5Dfb.seq
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>06166_13299_A5Df_ADH_rev_H02_23.ab1 1 TGGAAGGTTA TCAAGATAAG AATTTTCGTT TTAAACCTAA GGAGTCACTT TAAAATTTGT 61 ATACACTTAT TTTTTTTATA ACTTATTTAA TAATAAAAAT CATAAATCAT AAGAAATTCG 121 CTTATTTAGA AGTGGCGCGC CGGATCCTCT AGAGTCGAGA TCTCAGTGGT GATGGTGGTG 181 ATGTTTGTCA TCGTCGTCCT TGTAGTCCAT GGGTTTATCG TCATCATCCT TATAATCCAT 241 GATATCAACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA 301 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT 361 CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC 421 CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA 481 CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC 541 TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC 601 GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA 661 CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT 721 CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA 781 GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC 841 TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT 901 AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT 961 ACTTCGGGTA TACATATCAG TATATATTCT TATACCCGCA AAAATCAGCG CGCAAATACG 1021 CATACTGTTA TCTGGGCTTT TAGTAAGCCG GATCAGATCT TACGCCCCGC CTGGCACTCA 1081 TCGCAGTACT GTTGTAATTC ATTAAGCATC TGCCGACATG CAAGGCAATC ACAACCGGCA 1141 ATGGAAATTG GAACT // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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2022.05.18
GNP_filter3_RDBDEP_html_220516.pl