Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL2-HFF41

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14488_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14488_A6L7a.seq
>06194_14488_A6L7_nmt1Fw_D04_04_ABI08.ab1     1 TGAAATTTAA TTTAATCAAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA    61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT   121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA   181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC   241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC   301 CTATGCATCA CCACCATCAT CATATGGATT ATAAGGATGA TGACGATAAA CCCATGGACT   361 ACAAGGACGA CGATGACAAA GATATCATGA GATCTCGACT CTAGAGGATC CGGCGCGCCA   421 CTTCTAAATA AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA GTTATAAAAA   481 AAATAAGTGT ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA TTCTTATTCT   541 TGAGTAACTC TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG GTCGCTCTTA   601 TTGACCACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC TAGTGCCTCT   661 ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT GGAAGCTGAC   721 GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG TCGTCCTGAG   781 CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG ACCTTGCAAC   841 TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT TGAAGTGTCG   901 ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGGTGAGCC GCCACTGAGG   961 ACACGATCGG TTATGCTATG GACCACTTGG CCCTTACAGT TGGAAGAAGT TTCCTCGTAA  1021 TGGCCTCGAT TTGGCGGCCC GCGGGCCGAC TCCCAATCCA ATTGATCGAG TCGTGATCGA 1081 ATGAATTACA ACGTTTTTGC GACTA //
Primer: ADH-Forward Sequence file: RDB14488_A6L7b.seq
>06194_14488_A6L7_ADH-Forward_E04_05_ABI08.ab1     1 ATCTCCTTTT AATTGAAACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA    61 CTAGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT   121 TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT   181 GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC   241 GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG   301 TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC   361 GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG   421 TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACCTT CCAATTCATT AAATCGAGTC   481 CTCCATCAGA TGATTTACAA GGTTTTGCAT AGAATCCCTA AGTAAGAGAA GTTGTTAGTG   541 ATTTCTTCAA GTTTACTAAA ATTTACATAC AAATGGATGA TCTCTTCTCC ATTGTTTCCT   601 TTCTTCTTGC AAACGCGTTT TACAAAGAGA TGACATGCTA TTTTCTGATT ATTTTTTTCT   661 ATAGTTTTCT ATTTTGTACG TCCTTGGGAG TCCGAAATGT AAAATCGGGG TATGGTTAGT   721 AGCGGCAGCG TTCATTATGG TATATAAAAG ATAAAAAAGT AGGGGCAAGC GAGACAGAAT   781 GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAAATT GTATTTTAAT TTGTATTTTT   841 TGTAAATTTT ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTTAAAG AAAAACTTCA   901 ACGAAATGGT CAAAATTAAA GCTCCACAAA AATATTACCA ATTTTTAGGG AAAGCCGATT   961 CAGCAACTTT ATTCTTCCGG TTTGTAGGAT TTCTTTTTTC TTTCAATAAA TTCCAACCAT  1021 GCTACCTGGC CAATTGAATT CTTTCTCTCT AAATTGCTAT TAATTGAAAC CTAATGGATA 1081 TACTACCACA GCGTTTTTTG GACATTAAAT TGTTATGAAC TAT //
Primer: f1ori_R3 Sequence file: RDB14488_A6L7c.seq
>06194_14488_A6L7_f1ori_R3_F04_06_ABI08.ab1     1 GGCGTAGGGT CCTTGGCAGT ATGCGGGTGT GAATACCGCA CAGATGCGTA AGGAGAAAAT    61 ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC   121 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT   181 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC   241 TCGGGAGCGC TACCGTGAAT GGGCTCAACC AAACAATGAA CCTTTTCTTC TGATTTTCCT   301 ACCACACCGG AAAGGGAGGC AGAAGGCAAA AGGCCCAAGC TACCAGGAAT GACAGAAGCC   361 TCATCTGAAA TAATGTCACC AAACAAGTTG TCAGTCAAAA CAACACCGTT AAGTGTACGA   421 GGGCTCTTGA CCAAAAGCAT GGCTGCGGAG TCAATGAGCT GGTTTTTTAA GGTAAGGTGA   481 GGATATTCCT CCTTAAAAAT CTTAGCTACA GTCTTGCGCC AAAGACGAGA AGTTGCCAAA   541 ACATTAGCTT TGTCGAGTAA TGTGACGGGA GCAGGAGGGT TGGAAGTTTC AGCTAAGCGG   601 CCGCGGGCCC GAGTCTAACT CCTTAACCAC TCGGACATAG TGACTTATCT GACACTCATT   661 GTAAATTAAT ATATATATAG GCATTTTGTT TAGTTAAAGG TACTTAAGTA ATTAGTATAA   721 ACGAACCAAT TTTATAATCA GGAAGTTAAG TGAATGGTAG CACATGTCGT AAAAATTGTG   781 AATTTTTATT GAATAATATT TTAAATACAA GCCTTTCTAG ACTAGGTATA CTCATAAACA   841 TATGAGCAAA AGGATAGAGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT   901 TACTGAAAAA ATTAAATAAT GAGTACTAAA TGATAAAAAG CGCTCAGTAC AAGAAAGATT   961 GCAAAAATAT TGCATTCTTC ATGAATTTAA AGGTTTGCAT AATAAGGGCA TATTTGAAAG  1021 TAATAGTACT AAAACAGCCA GGTTAAGCCG AGATATTAGA GTTATTATTC GCAGACATGT 1081 GACCATTAGA TTAAAAAGAC TTTGTAAGAC //
Primer: Reverse2 Sequence file: RDB14488_A6L7d.seq
>06194_14488_A6L7_Reverse2_H09_24_ABI24.ab1     1 CATAAATCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG    61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT   121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT   181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA   241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT   301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG   361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA   421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG   481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT   541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG   601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG   661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT   721 CGGTATGCTT TCTCAAACCA TTCTAAGGTT TTCTCTGTGT AGGAAACCAG TAGCCCAAAG   781 AGCCTTTTGG AAGACATTTC AACCCAAAAG CAAAGAGACC ACGTCCCAAA GGTAAACCAA   841 CTTTCTTTGA GGCCCTTTGT ATAATACCCC TCGCCCTGGC ACTGTATGGC AATTTTGTGA   901 TATGAGCCCC AAGAAGCAAT TTTGTACACA CCAGATGCCT ATTTGGTAAC TTTGACCGGT 961 ATTTTCCAAT TGTTCGGCGA AAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl