Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL2-HFF81

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (low strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14489_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14489_A6L7a.seq
>06195_14489_A6L7_nmt1Fw_F01_16_ABI24.ab1     1 AACATTTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG    61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG   121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG   181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC   241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCTATGC   301 ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG GACTACAAGG   361 ACGACGATGA CAAAGATATC ATGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA   421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA   481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA   541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC   601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTGC CTCTATTGAT   661 GCCTATGGAA CCCCTTTGAC TGACGAGACT GTGAAGGCTT GTTTGGAAGC TGACGGTGTT   721 CTTTTGGGTG CCGTTGGTGG TCCTGAATGG ACCAACCCCA ATTGTCGTCC TGAGCAAAGG   781 TTTATTGAAG CTTCGTAAGA GTATGGGTGT TTGGGCCAAC CTTCGACCTT GCAACTTTGC   841 CAGCAAGTCT TTAGTCAAGT ACAGCCCTTT GAAGCCCTGA AATCGTTGAA GGTGTCGATT   901 TTTGTGTTGT ACGAGAACTT ACTGGAGGTT GTTACTTTGG TGGAGCGCAC TGAAGACACG   961 GGATCGGGTT ATGCTATGGG ACACTGGCCT TACAGTTTGG AGGAGGTTTT CTCGTATGCT  1021 CGATTGGGCG GCGCGGGCCG AAGCATTCAA ATCATCAAGT CGAAGTTCGT CAATCAGAAT 1081 GATTTTTACC AG //
Primer: ADH-Forward Sequence file: RDB14489_A6L7b.seq
>06195_14489_A6L7_ADH-Forward_G01_19_ABI24.ab1     1 TGGTCACTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT    61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG   121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT   181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA   241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT   301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC   361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT   421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT   481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT   541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT   601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT   661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG   721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG   781 ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTTGT   841 AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT TAGAGAAAAC TTCAACGAAA   901 TGTCAAAATA AGCTCAGCAA AATATACAAT TTTTAGGGAA AGCGATCAGC AACTTTATTT   961 CTCCGTTTGT AGATTTCTTT TTCTTTCATA AATTTCCTAC AATGTAAACT GCCCAATTTG  1021 GATTTTTCTC TAGATGGCTA ATAAATCAAC CTTATTGAAT ATATACCAGT TTTGATAAAT 1081 TGTAAACTTC TTTACTTCGA //
Primer: f1ori_R3 Sequence file: RDB14489_A6L7c.seq
>06195_14489_A6L7_f1ori_R3_H01_22_ABI24.ab1     1 GGCATGGCTC AGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA    61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT   121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA   181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA   241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA   301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT   361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC   421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT   481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA   541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG   601 GCCCGAGTCT AACTCCTTAA CCACTCGGAC ATAGTGACTT ATCTGACACT CATTGTAAAT   661 TAATATATAT ATAGGCATTT TGTTTAGTTA AAGGTACTTA AGTAATTAGT ATAAACGAAC   721 CAATTTTATA ATCAGGAAGT TAAGTGAATG GTAGCACATG TCGTAAAAAT TGTGAATTTT   781 TATTGAATAA TATTTTAAAT ACAAGCCTTT CTAGACTAGG TATACTCATA AACATATGAG   841 CAAAAGGATA GAGGAGATTA CATTGCATCT TCTACAATTT ATTTATTGCC CCTTTACTGA   901 AAAAATTAAT AATGAGTACT AAATGAATAA AAAGCGCCTC AGTACAAGAA AAGATTTGCT   961 AAATATGCCA TTCCTTCCAT GCATCAAGTG GCATAATAAG CATATTGGCA GTATAGGTAC 1021 CTTAAACTGC AGTAGTCCGA AATTATTGA //
Primer: Reverse2 Sequence file: RDB14489_A6L7d.seq
>06195_14489_A6L7_Reverse2_A02_02_ABI24.ab1     1 CGGGAATCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG    61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT   121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT   181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA   241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT   301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG   361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA   421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG   481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT   541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG   601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG   661 TTATGTAGTC GCTTTGAAAG GTTAGGAAAT CGACGACCAG CTATAAAGCC AAAGCAAAAA   721 TCGGTATGCT TCTCAAACCA TTCTAAAGGT TTTCTCTGTG TAGGAACCCA GTAGCCAAGA   781 GCCTTTGGGA AGACATTTCA GCCCAAAAGC AGAGACCACG TCCCCAAAGG TAAACCAACT   841 TCTTTGAGCC TTGTATAATA CCCTCGCTGC ACTGTATGGC AAATTTGTGA ATATGAGCCA   901 GGAGCATTTG TACACACCAG ATGCATATGG TAGCCTGAAC GTATTTCGAA TGTCCTGCAA 961 ATAGCGAATC GTCAGGATAG AATCGATGCA TTAACTTAAA GCCACCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl