Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL2-GFH1

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14464_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14464_A6L7a.seq
>06202_14464_A6L7_nmt1Fw_A05_02_ABI24.ab1     1 TGGTATTTAT TAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG    61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG   121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT   181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT   241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT   301 CCGGATATCA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA   361 TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA   421 ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG   481 CCAACACTTG TCACTACTTT CTGTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC   541 ATGAAACAGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GCTATGTACA GGAAAGAACT   601 ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT   661 ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT   721 GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA   781 AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA   841 CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC   901 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC   961 ATGGGTCCTT CTTTGAGTTT GTAACAGCTG CTGGGATTTA CACATGGCAT GGATGAACTA  1021 TACAAACCCA ATGGGACTAC AGACGACGAT GACCAACATT CAACACCATT CACACTGAGA 1081 TCTCGAACTC TAGAGGGAAT CGGCGCCGCC CACCTTTTCT TAATGAG //
Primer: ADH_rev Sequence file: RDB14464_A6L7b.seq
>06202_14464_A6L7_ADH_rev_B05_05_ABI24.ab1     1 CGGGGATTCT CAAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA    61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT   121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG   181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG TTTGTATAGT TCATCCATGC CATGTGTAAT   241 CCCAGCAGCT GTTACAAACT CAAGAAGGAC CATGTGGTCT CTCTTTTCGT TGGGATCTTT   301 CGAAAGGGCA GATTGTGTGG ACAGGTAATG GTTGTCTGGT AAAAGGACAG GGCCATCGCC   361 AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG CTTCCATCTT CAATGTTGTG   421 TCTAATTTTG AAGTTAACTT TGATTCCATT CTTTTGTTTG TCTGCCATGA TGTATACATT   481 GTGTGAGTTA TAGTTGTATT CCAATTTGTG TCCAAGAATG TTTCCATCTT CTTTAAAATC   541 AATACCTTTT AACTCGATTC TATTAACAAG GGTATCACCT TCAAACTTGA CTTCAGCACG   601 TGTCTTGTAG TTCCCGTCAT CTTTGAAAAA TATAGTTCTT TCCTGTACAT AGCCTTCGGG   661 CATGGCACTC TTGAAAAAGT CATGCTGTTT CATGTGATCT GGGTATCTTG AAAAGCATTG   721 AACACCATAA CAGAAAGTAG TGACAAGTGT TGGCCACGGA ACAGGTAGTT TTCCAGTAGT   781 GCAAATAAAT TTAAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA   841 CAGAAAATTT GTGCCCATTA ACATCACCCA TCTAATTCAA CAAGAATTGG GACACTCCAG   901 TGAAAGTTCC TTCCTCCTTT ACTCATGATA TCCCGGATCC AGGCCCTGTC GACCGCTAGC   961 TTTAACAAAG CGACTATAAG TCAGAAAGTG AGATGGAGAT TGAAAATAAT TTAATTCCAA  1021 CTTATCATGA TGGAATATGG CCAGGAATCC TCTCCTTATA GATCCTTCCA TGATTCGGAT 1081 AACCAGGTCC ATTATCCGGG T //
Primer: ADH-Forward Sequence file: RDB14464_A6L7c.seq
>06202_14464_A6L7_ADH-Forward_C05_08_ABI24.ab1     1 TGGTCATTTA TTGGACACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC    61 TAGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT   121 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG   181 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG   241 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT   301 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG   361 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT   421 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACCTTC CAATTCATTA AATCGAGTCC   481 TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA   541 TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT   601 TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA   661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA   721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAT GGGCAAGCGA GACAGAATGG   781 GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAAATTT GTATTTTTTG   841 TAATTTTATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA CTTTCAACGA   901 ATGTCAAAAA TAAGCTCAGC AAAAATATAC AATTTTAGGA AAGGCGATCA GCCACTATCT   961 CCGTGTAGAT TCTTTTCCTC ATAATTCAAC ATGTACTGCG ACTGAATTTT CTCTAGATGC 1021 TATAAATTGA ACTATGCATT ACATACCTCA GGTTGTGCAT //
Primer: f1ori_R3 Sequence file: RDB14464_A6L7d.seq
>06202_14464_A6L7_f1ori_R3_D05_11_ABI24.ab1     1 GGCATGCGCT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT    61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC   121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC   181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG   241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC   301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG   361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT   421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT   481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG   541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG   601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT   661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC   721 AATTTTATAA TCAGGAAGTT AAGTGAATGG TAGCACATGT CGTAAAAATT GTGAATTTTT   781 ATTGAATAAT ATTTTAAATA CAAGCCTTTC TAGACTAGGT ATACTCATAA ACATATGAGC   841 AAAAGGATAG AGGAGATTAC ATTTGCATTC TTTCTAACAA ATTTTATTTT ATTGCCCTTT   901 ACTGGAAAAT TTAAATAATG AGTACTAAAT GAATAAAAAA GCGCCTCAGT ACAAAGAAAA   961 GATTGCAAAA TTATTGCATT CTTCAATGAA TCAAGTTGCA TATAAGCATA ATTGGAGGTT 1021 ATTAGTTACT GAACAGGCAG TAGCTAGATT ATTGGCAT //
Primer: Reverse2 Sequence file: RDB14464_A6L7e.seq
>06202_14464_A6L7_Reverse2_E05_14_ABI24.ab1     1 CGTAAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG    61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT   121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT   181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA   241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT   301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG   361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA   421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG   481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT   541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG   601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG   661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT   721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC   781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT   841 TGAGGCCTTG TATAATACCC TCGCCTGGCA CTGTATGGCA ATTTGTGATA TGAGCCCAAG   901 AAGCAATTTT GTACACACCA GATGCATATT GTAGCTTGAC GGTATTTCCC AATGTCTGCG   961 AAATTTGCGA TCCCTCAAGA TAAGAAAACG ATGCTTTTTT ACCCTAAAGC CACCAGTTTT  1021 TTCTAACCAT ATCCCTGCTC GGAATTCCTT CGACCACGTT CGAATATTGC TATCTGATAC 1081 ACAGGAACTT AGGTCCAAAT TTTTAATTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl