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pZF-Runxb3

Plasmid clone of zebrafish runx3 (runxb) cDNA

Catalog number RDB06423
Resource name pZF-Runxb3
Alternative name Zebrafish runx3 (runxb) alternatively spliced transcript 3
Clone info. Zebrafish (Danio rerio) cDNA for runt-related transcription factor runx3 (previously called runxb), alternatively spliced transcript 3, a complete coding sequence.
Comment The translational orientation of the inserted runx3 cDNA sequence is from T7 promoter to T3 promoter of the vector. The inserted sequence contains 60bp of cloning artifact, which is 780 to 839th of the cDNA sequence, between the EcoRI site of the vector and the start of the cDNA sequence (1 to 1731th). The XbaI sites of the plasmid may not be digested with XbaI restriction enzyme because of DNA methylation by the host JM109.
Vector backbone pCR-Script SK(+) (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r, Kan^r
Gene/insert name Zebrafish (Danio rerio) runx3_(runxb) cDNA
Reference of insert sequence AB043790 (DDBJ accession number)
Depositor|Developer Kataoka, Hiroko |
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External Database
Zebrafish (Danio rerio) runx3_(runxb)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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          RDB06423 pZF-Runxb3 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence RDB06423z.seq
          Remarks, protocol and/or map (pdf) RDB06423.pdf

          Original reference

          original Kataoka, H., Cloning and embryonic expression patterns of the zebrafish Runt domain genes, runxa and runxb. Mech. Dev., 98, 139-143 (2000). PMID 11044618.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Kataoka, H., Cloning and embryonic expression patterns of the zebrafish Runt domain genes, runxa and runxb. Mech. Dev., 98, 139-143 (2000). PMID 11044618.

          2022.05.18

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