Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGFH81

S. pombe expression vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14477_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14477_A6L7a.seq
>06591_14477_A6L7_nmt1Fw_G11_20_ABI24.ab1     1 ATGAATTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG    61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG   121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG   181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC   241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCGGATA   301 TCATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG   361 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG   421 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC   481 TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC   541 AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA ACTATATTTT   601 TCAAAGATGA CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG   661 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA   721 AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG   781 GAATCAAAGT TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG   841 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT   901 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGGTC   961 CTTCTTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGG CATGGATGAA CTATACAACC  1021 CATGGGACTA CAGGACGACG ATGACAACAT CACACATCAC ACTGAAGATC TCGAGCCGGC 1081 CGATCGCTAA CTAACTTAAA GGGGCAAAAA AATGA //
Primer: nmt1ter_R Sequence file: RDB14477_A6L7b.seq
>06591_14477_A6L7_nmt1ter_R_H11_23_ABI24.ab1     1 GATTTCTAAG TAAACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT    61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG   121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA   181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT   241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA   301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA GTGGTGATGG   361 TGGTGATGTT TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA   421 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG   481 GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG   541 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA   601 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG   661 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT   721 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAAACAAGG GTATCACCTT CAAACTTGAC   781 TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA   841 GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA   901 AAAGCATTGA ACACCCATAA CAGAAAGTAG TGACAAGTGT TGGGCCACGG AAACAAGGTA   961 GGTTTTCCAG TAGTTGCAAA TAATTTTAAT GGGTAAAGGT TTTCCCGTTA TGTTTGCATC  1021 ACCCTTTCAC CCTTCTCCAC TGGAACAGAA AAATTTGTGT GCCCAGTACC ATCACCAATC  1081 GAATTTCCAA CGAGGATTGG GGACCACTCC AGGTGGAAGA AGTTCCTTCT TCCTTTTTAC 1141 CTGCTCAACA TG //
Primer: f1ori_R3 Sequence file: RDB14477_A6L7c.seq
>06591_14477_A6L7_f1ori_R3_B12_06_ABI24.ab1     1 GCAATTGGGG GCTTGGAGTA TGCGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC    61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG   121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG   181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT   241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT   301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG   361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT   421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG   481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT   541 AGGGGCGAGC CGAGACAAAA AGGGGATACA AGGGCATCGT CTATAATTAT AGCTAAAAAA   601 AAGTATTTTA ATTTGTATTT TTTGTAATTT TATTTCTCAT TGTTTTACTT AAAATGAATG   661 CGAATTAGAG AAAAACTTTC TTCGAAATGT CAAAATAAGC TCAGCAAAAT ATACAATTTT   721 ATGGGAAAGC GATCAGCAAC TTATTCTCCG TTGTAGATTC TTTTTCTTCA TAAATTCCAA   781 CAATGTAACT GCCAATTGAT TTTTCTCTAG ATGCTAATAA ATTTGAAACT AATGAATATA   841 ATAACACAGG TTTTGATAAA TTGTAAACTC TTACTCAAAA AAGAATGTTA AACTAAATTA   901 ACTTTTTATA AGCCCATGGC GTTAAAGAGG ATAGTTAAAT CTACAATTTT TTAATTTATG   961 TCACATTGTC TTGCAAATAA TTCTATAATT TCGCTAACTG CTGTTAGAAC ATATACCTCA  1021 ATTGGCTAAA TGGCCACTAA TTCCAGAGAA CTGCAATTGC ACTTCTGACT GAGCGCTTAA 1081 TCA //
Primer: LEU2pro_F Sequence file: RDB14477_A6L7d.seq
>06591_14477_A6L7_LEU2pro_F_A12_03_ABI24.ab1     1 AAACTTGCAG CTGACATATT ATTTAAGGAC CTATTGTTTT TTCCAATAGG TGGTTAGCAA    61 TCGTCTTACT TTCTAACTTT TCTTACCTTT TACATTTCAG CAATATATAT ATATATTTCA   121 AGGATATACC ATTCTAATGT CTGCCCCTAA GAAGATCGTC GTTTTGCCAG GTGACCACGT   181 TGGTCAAGAA ATCACAGCCG AAGCCATTAA GGTTCTTAAA GCTATTTCTG ATGTTCGTTC   241 CAATGTCAAG TTCGATTTCG AAAATCATTT AATTGGTGGT GCTGCTATCG ATGCTACAGG   301 TGTTCCACTT CCAGATGAGG CGCTGGAAGC CTCCAAGAAG GCTGATGCCG TTTTGTTAGG   361 TGCTGTGGGT GGTCCTAAAT GGGGTACCGG TAGTGTTAGA CCTGAACAAG GTTTACTAAA   421 AATCCGTAAA GAACTTCAAT TGTACGCCAA CTTAAGACCA TGTAACTTTG CATCCGACTC   481 TCTTTTAGAC TTATCTCCAA TCAAGCCACA ATTTGCTAAA GGTACTGACT TCGTTGTTGT   541 CAGAGAATTA GTGGGAGGTA TTTACTTTGG TAAGAGAAAG GAAGACGATG GTGATGGTGT   601 CGCTTGGGAT AGTGAACAAT ACACCGTTCC AGAAGTGCAA AGAATCACAA GAATGGCCGC   661 TTTCATGGCC CTACAACATG AGCCACCATT GCCTATTTGG TCCTTGGATA AAGCTAATGT   721 TTTGGCCTCT TCAAGATTAT GGAGAAAAAC TGTGGAGGAA ACCATCAAGA ACGAATTCCC   781 TACATTGAAG GTTCAACATC AATTGATTGA TTCTGCCGCC ATGATCCTAG TTAAGAACCC   841 AACCCACCTA AATGGTATTA TAATCACCAG CAACATGTTT GGTGACATCA TCTCCGATGA   901 AGCCTCCCGT TATCCCAGGT TCCCTTGGGG TTTGTTGCCA TCTGCGTCCT TGGGCCCTCT   961 TTGCCCAGAC AAGAACACCG CATTTTGGGT TTGGTACGGA TCAATGCCCA CGGGTTCTGC  1021 TCCCAGAATT TTGCCCAAAG AATAAGTCAA ACCCTATCGC CCACTATTCT TGTCTGCTTG 1081 CAAATGATGT GAATGGTCAT GGACCTTGGC CCTGAGAGGT TAGAGGCCAT TTGAGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl