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pCMFlag_hsTP53

Expression vector of human p53.

Catalog number RDB06754
Resource name pCMFlag_hsTP53
Alternative name SET-0136_2-5
Clone info. Expression vector of human p53. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gaggagccgcagtcagatcc; reverse primer: tcagtctgagtcaggcccttc.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human tumor protein p53 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human tumor protein p53
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB06754.pdf

          Distribution information

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          RDB06754 pCMFlag_hsTP53 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsTP53 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06754).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13744_A5Lop1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13744_A5Loa.seq check
          >06754_13744_A5Lo_CMV-Forward_A03_03.ab1
              1 TAATCAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA
            181 GGCGGCCGAG GAGCCGCAGT CAGATCCTAG CGTCGAGCCC CCTCTGAGTC AGGAAACATT
            241 TTCAGACCTA TGGAAACTAC TTCCTGAAAA CAACGTTCTG TCCCCCTTGC CGTCCCAAGC
            301 AATGGATGAT TTGATGCTGT CCCCGGACGA TATTGAACAA TGGTTCACTG AAGACCCAGG
            361 TCCAGATGAA GCTCCCAGAA TGCCAGAGGC TGCTCCCCCC GTGGCCCCTG CACCAGCAGC
            421 TCCTACACCG GCGGCCCCTG CACCAGCCCC CTCCTGGCCC CTGTCATCTT CTGTCCCTTC
            481 CCAGAAAACC TACCAGGGCA GCTACGGTTT CCGTCTGGGC TTCTTGCATT CTGGGACAGC
            541 CAAGTCTGTG ACTTGCACGT ACTCCCCTGC CCTCAACAAG ATGTTTTGCC AACTGGCCAA
            601 GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG CCCGGCACCC GCGTCCGCGC
            661 CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT GTGAGGCGCT GCCCCCACCA
            721 TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG CATCTTATCC GAGTGGAAGG
            781 AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT CGACATAGTG TGGTGGTGCC
            841 CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC CACTACAACT ACATGTGTAA
            901 CAGTTCCTGC ATGGGCGGCA TGAACCGGGA GGCCCATCCT CACCATCATC ACACTGGAAG
            961 ACTCCAGTGG TAATCTACTG GGACGGAACA GCTTTGAGTG CGTGTTTGTG CCTTGTCCTG
           1021 GAGAGACCGG GCGGCACAGA AGGAAGGAGA ATCTTCCGGC AAGAAAAGGG GAGCCTTCAC
           1081 CACGGGAGCC TGCCTCCAAG GGAAGCACTT AAGACGGAGC CACTGCCCAC AACTCCAGCT
          1141 TCCTCTCTCC CCAGCCAAGG AGGAAACACA CTTGGCATAT GAAGAAATAT AAT
          //
          Primer: BGH_rev
          Sequence file: RDB13744_A5Lob.seq check
          >06754_13744_A5Lo_BGHrev_B03_06.ab1
              1 GTCCTGCATT GGTGAACTAT AGAATAGGGC CCTCTAGATG CATGCTCGAG CGGCCTCAGT
             61 CTGAGTCAGG CCCTTCTGTC TTGAACATGA GTTTTTTATG GCGGGAGGTA GACTGACCCT
            121 TTTTGGACTT CAGGTGGCTG GAGTGAGCCC TGCTCCCCCC TGGCTCCTTC CCAGCCTGGG
            181 CATCCTTGAG TTCCAAGGCC TCATTCAGCT CTCGGAACAT CTCGAAGCGC TCACGCCCAC
            241 GGATCTGAAG GGTGAAATAT TCTCCATCCA GTGGTTTCTT CTTTGGCTGG GGAGAGGAGC
            301 TGGTGTTGTT GGGCAGTGCT CGCTTAGTGC TCCCTGGGGG CAGCTCGTGG TGAGGCTCCC
            361 CTTTCTTGCG GAGATTCTCT TCCTCTGTGC GCCGGTCTCT CCCAGGACAG GCACAAACAC
            421 GCACCTCAAA GCTGTTCCGT CCCAGTAGAT TACCACTGGA GTCTTCCAGT GTGATGATGG
            481 TGAGGATGGG CCTCCGGTTC ATGCCGCCCA TGCAGGAACT GTTACACATG TAGTTGTAGT
            541 GGATGGTGGT ACAGTCAGAG CCAACCTCAG GCGGCTCATA GGGCACCACC ACACTATGTC
            601 GAAAAGTGTT TCTGTCATCC AAATACTCCA CACGCAAATT TCCTTCCACT CGGATAAGAT
            661 GCTGAGGAGG GGCCAGACCA TCGCTATCTG AGCAGCGCTC ATGGTGGGGG CAGCGCCTCA
            721 CAACCTCCGT CATGTGCTGT GACTGCTTGT AGATGGCCAT GGCGCGGACG CGGGTGCCGG
            781 GCGGGGGTGT GGAATCAACC CACAGCTGCA CAGGGCAGGT CTTGGCCAGT TGGCAAAACA
            841 TCTTGTTGAG GGCAGGGGAG TACGTGCAAG TCACAGACTT GGCTGTCCCA GAATGCAAGA
            901 AGCCCAGACG GAAACCGTAG CTGCCCTGGT AGGTTTTCTG GGAAAGGGAC AGAAGATGAC
            961 AGGGGCCAGG AGGGGGCTGG GTGCAGGGCC CGCCGGGTGT AGAGCTGCTG GGTGCAGGGC
           1021 CACGGGGGGG AGCAGCCTCT GGCATTCTGG GAGCTTCATC TGGGACCTGG GGTCTCAGTG
           1081 ACCATGATCA ATATCGTCCG GGACGCATCC AATTCATTCA TGCTGGGACG GCAAGGGGGA
          1141 ACAGAAACGG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13744_A5Loc.seq check
          >06754_13744_A5Lo_SV40pro_F_V2_E12_15.ab1
              1 GAACTTTTTT GGAGCTAGGC TTTTGCAAAA GCTCCCGGGA GCTTGGATAT CCATTTTCGG
             61 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC
            121 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT
            181 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT
            241 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG
            301 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG
            361 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC
            421 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC
            481 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA
            541 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA
            601 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG
            661 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG
            721 TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC
            781 TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTTCCTCGT GCTTTACGGT ATCGCCCGCT
            841 CCCGATTCGC AGCGCATCGC CTTCTATCGC CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
            901 CTGGGGGTTC GAAATGACCG AACCAGCGAC GCCCAACCTG CATCACGAGA TTTCGATCAA
            961 CGGCGCTTCC TATGAAAGGT GGCTCCGATC GTTTTCCGGA CGCGCTGATG ATCTCAGCGC
          1021 GGCAATCTCA TGCTGAGAGG T
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Shimizu, H., NF-kappaB plays an important role in indoxyl sulfate-induced cellular senescence, fibrotic gene expression, and inhibition of proliferation in proximal tubular cells. Am. J. Physiol. Cell Physiol. 301 (5): C1201-12 (2011). PMID 21832251.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl