Resource data sheet
Prev. |  Lentiviral vector plasmid > 
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-MCS-IRES2-hKO1

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.

Catalog number RDB07915
Resource name CSII-EF-MCS-IRES2-hKO1
Clone info. Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-MCS ()
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence (full) RDB07915zzk02.seq provided by the depositor
            Remarks, protocol and/or map (pdf) RDB07915.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose [Word]
            Please visit Ordering instruction.[link] 
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB07915 CSII-EF-MCS-IRES2-hKO1 DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSII-EF-MCS-IRES2-hKO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07915).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Lentiviral plasmid list (English text)
            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16940_A9Cip1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F (Pr0168)
            Region: EF-1 alpha pro, IRES2
            Sequence file: RDB16940_A9Cig.seq check
            >07915_16940_A9Ci_3_EF1a-C_F_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTCNTTCT CNAGCCTCAG ACAGTGGTTC
               61 AAAGTTTTTT TCTTCCNTTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA
              121 GCGGCCGCGG ATCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT
              181 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
              241 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC
              301 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA
              361 AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC
              421 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC
              481 ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC
              541 AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA
              601 TCTGGGGCCT CGGTNCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG
              661 CCCCCCGAAC CACNGGGANG TGGTTTTCCT TTNAAAAACA CGATGNTAAT ATGGCCACGG
              721 GGNTNCCCAC CATGGTGAGC GTGNTCAAGC CCGANATGAA GATGAAGTAC TTCNTNGNNG
              781 GNANCGTNNN CGGNNNCNAN TTNNNCGTGG ANGNNGAGGG CACCNGNNNA NCCCTNCNAN
              841 GGNNNCCNNN ANNTGANCCT NANGNNNANA NTNGNNNNAG GGCNGNCCCN NNCCNTTNNN
            901 CNNNNNNNTN NNNNNNCNCN NNTTNNNNTN NNNNNNNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE, hKO, IRES2
            Sequence file: RDB16940_A9Cih.seq check
            >07915_16940_A9Ci_3_PRE_rev_B10_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNNNN NNNNCNNCAT AGTTAAGAAN ACCAGTCAAT CTTTCNCAAA
               61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGCCTG
              121 CAGTCAGCAG TGGGCCACGG CGTCCTCCAC CAGCTCGGTG ATGTTGCCCT CGGTCTTCCT
              181 CACCAGCCTG TGGCCGATGA AGTGGCTCTG GGGCATCTTC AGGATCTTCT TGGCGGCCTT
              241 GTAGGTGGTC TTGAACTGGC ACTTGTGGTT GCCGCCGCCG GCCAGCTTCA GGTACATGGT
              301 CACGTCGCCC TTCAGCACGC CGTCGCAGGT GGTGATCTTC TCGGTGCTGG GCTCCCAGTC
              361 GCTGCTCTGG TTCTGCATCA CGGGGCCGTC GGCGGGGAAG TTCACGCCCA CGAACTTGCT
              421 CTTGTGCTCG AAGCAGTTGC CCCTCAGGCT GATGTGGGCG CTCACGGCGG CGAAGCCGCC
              481 GTCCTCGAAC TGGAGGCTCC TCTCCCAGCT CAGGCCCTCG GGGAAGGCCT GCTTGAAGTA
              541 GTCGGGGATC TCCTCGGGGT ACTTGGTGAA GGGCCTGTGG CCGTAGCAGA AGGTGTGGCT
              601 CACCAGGTCG AAGCTGAAGG GCATGGGGCC GCCCTTGGCC ATTGTCACCC TCAGGGTCAT
              661 CTCCTGGTGG CCCTCGTAGG GCTTGCCGGT GCCCTCGCCC TCCACGGTGA ACTCGTGGCC
              721 GTTCACGCTG CCGTCCATGA AGTACTTCAT CTTCATCTCG GGCTTGATCA CGCTCACCAT
              781 GGTGGGGATC CCCGTGGCCA TATTATCATC GTGTTTTTCA AANGAAAACC ACGTCCCCNN
              841 GGTTCGGGGG GGNCTANANG TTTTTTTAAC CTCNANTAAN CACATGTAAA GCATGTGTAN
              901 CNANGCCCCA NNTCANATNC NNTACAANGG GGGTNNNNTC TGGNNATCCT TCANCCCNNT
            961 NNNTGAATAN NCNTNNANNA NNNCNNTTTG NNNNTTNNNN NNNNNN
            //
            Primer: IRES-F (Pr0036)
            Region: IRES2, hKO 5'
            Sequence file: RDB16940_A9Cii.seq check
            >07915_16940_A9Ci_3_IRES-F_F10_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTNNG TCGANGTTNN NAAACGTCTA GGCCCCCCGA ACCACGGGGN
               61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC GGGGATCCCC ACCATGGTGA
              121 GCGTGATCAA GCCCGAGATG AAGATGAAGT ACTTCATGGA CGGCAGCGTG AACGGCCACG
              181 AGTTCACCGT GGAGGGCGAG GGCACCGGCA AGCCCTACGA GGGCCACCAG GAGATGACCC
              241 TGAGGGTGAC AATGGCCAAG GGCGGCCCCA TGCCCTTCAG CTTCGACCTG GTGAGCCACA
              301 CCTTCTGCTA CGGCCACAGG CCCTTCACCA AGTACCCCGA GGAGATCCCC GACTACTTCA
              361 AGCAGGCCTT CCCCGAGGGC CTGAGCTGGG AGAGGAGCCT CCAGTTCGAG GACGGCGGCT
              421 TCGCCGCCGT GAGCGCCCAC ATCAGCCTGA GGGGCAACTG CTTCGAGCAC AAGAGCAAGT
              481 TCGTGGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGCA GAACCAGAGC AGCGACTGGG
              541 AGCCCAGCAC CGAGAAGATC ACCACCTGCG ACGGCGTGCT GAAGGGCGAC GTGACCATGT
              601 ACCTGAAGCT GGCCGGCGGC GGCAACCACA AGTGCCAGTT CAAGACCACC TACAAGGCCG
              661 CCAAGAAGAT CCTGAAGANG CCCCAGAGCC ACTTCATCGG CCACAGGCTG GTGAGGAAGA
              721 NCGANGGCAA CATCACCGAN CTGGTGGAGG ACGCCGTGGC CCACTGCTGA CTGCAGGCTA
              781 GAGTTAACAT CGAGGGATCN AGCTTATCGA TNATCAACCT CTGGNNTACN AAATTTGTGA
              841 AAGANTGACT GGNATTCTNA ACTATGTTGC TCCNTTTACG CTATGNNGNN ACGCTGCTTN
              901 NANGNCTTTG NATCATGCTA TNGNNNCCCG TANNNNTTNN ATTTTNNNNN NNCNTNNNAN
            961 ANNNCNNNNN NNNNN
            //
            Primer: CMV_Forward (Pr0016)
            Region: CMV pro, 5' LTR, HIV-1 psi
            Sequence file: RDB16940_A9Cij.seq check
            >07915_16940_A9Ci_3_CMV-Forward_C10_07_ABI24.ab1
                1 NNNNNNNNNN NNNNTTGNNG CNNTGGGCGG TAGGCGTGTN CGGTGGGAGG TCTATATAAG
               61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
              121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
              181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
              241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
              301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
              361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
              421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
              481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
              541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
              601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
              661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
              721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT
              781 CTTCAGANCT GGNNGANGAN ATATGAGGGA NAATTGGAGA AGTGAATTAT ATAAATATNA
              841 AGTAGTAAAA ATTGAACCNT TANGANNAGC NCCCACNNNG GNNAAGANAA NNNNNGNNGN
              901 ANANNNNAAA AAAGANCANN NGGNANTNNG NNNNNTNNNT NNNNNNGGNN TNNNTNNGNN
            961 NNNNNNNN
            //
            Primer: EF1a-N_R (Pr0024)
            Region: EF-1 alpha pro, cPPT_CTS
            Sequence file: RDB16940_A9Cik.seq check
            >07915_16940_A9Ci_3_EF1a-N_R_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNGGNGNN GTGCGCTCTG CCCNCTGNCG GGCACCGGAN CCTCACGAAG
               61 CTTGAATTAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT
              121 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
              181 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
              241 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
              301 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
              361 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
              421 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
              481 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
              541 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
              601 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
              661 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCNCN
              721 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG
              781 CTGCTTGATG CCCCAGACTG TGANTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAN
              841 NAANNNGNTC TGCTGCTGCA CTATACCAGA NAATAATTGT CTGGNCTGTA CCNNCAGCGN
              901 CNTTNACNCN NCGCCCATAN NGCTTNCNGN NNCTNNNNNN ANCCCAANNN NNNAAANNTN
            961 NNNNTNCCCN CTGCNNNTTT NNNNNNNNNN
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGH pA, 3' LTR
            Sequence file: RDB16940_A9Cil.seq check
            >07915_16940_A9Ci_3_BGH_rev2_D10_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGG GGGNNGGNNN NNNACNGATG GCTGGCAACT AGAAGGCACA
               61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
              121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
              181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
              241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
              301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
              361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
              421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
              481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
              541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
              601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
              661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
              721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
              781 ACNGTATCGA TGCGGGGANG CGGCCCNAAG GGAGATCCGA CTCGTCTGAN GGCGANNNNG
              841 AAGANGCGGA AGANGNNGCA NANCCNNNNN CNNNNCNCNG GAAGGAAGGN CCGCTGGATT
              901 GANGGNCNNA NNGNANNNNN CNNAANGNNN TCCCGCNCAN AANNNNNNGN NGNNNNNNCN
            961 NNNNNNNNN
            //
            Primer: SV40pro_F (Pr0110)
            Region: SV40 pro_ori, EM7 pro, Zeo, SV40 pA
            Sequence file: RDB16940_A9Cim.seq check
            >07915_16940_A9Ci_3_SV40pro_F_G10_19_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GNTTTTTTGG ANGCCTAGGC TTTTGCAAAA
               61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
              121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
              181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
              241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
              301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
              361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
              421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
              481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
              541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
              601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
              661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
              721 CATCACAAAT TTCACNAATA AAGCATTTTT TTCACTGCAT TCTANNNGTG GTTTGTCCNA
              781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCNACC TCTAGCTAGA GCTTGNCGTA
              841 NTCATGGTCA TAGCNGNTTN CNTGTGTGAA ANTGTTATNC NNCTCACAAT TNCNCACNAN
              901 CATACGANCC NGGANNCATA AAGTGTAAAN CCNGGGGGTG NCNNANNNNT GNNCTANNNN
            961 NCNTTNANTN GNNTTGCNCN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619. [link to RRC of NBRP]
            user_report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640. [link to RRC of NBRP]
            user_report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243. [link to RRC of NBRP]
            user_report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Adv. 1: 1440-1451 (2017). PMID 29296785. [link to RRC of NBRP]
            user_report Lee, J., Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation. Cell Stem. Cell, 5, 76-86 (2009). PMID 19570516. [link to RRC of NBRP]

            2023.11.16

            GNP_filter3_RDBDEP_html_231027.pl