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pcDNA3.1-His-Gli2 (#RDB08065)

Expression vector of mouse Gli2 cDNA


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sasaki, H., Development, 126, 3915-3924 (1999). PMID 10433919. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse Gli2 cDNA.
Comment This resource contains a T>C substitution at nucleotide position 1287 in NM_001081125.1, resulting in a p.Ile313Thr substitution.
Vector backbone pcDNA3.1/HisB (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Growth conditions 37℃, LB+Amp
Gene/insert name Mouse Gli2 cDNA
Depositor|Developer Sasaki, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee
RDB08065 pcDNA3.1-His-Gli2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-His-Gli2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08065).

Reference section:

Sasaki, H., Nishizaki, Y., Hui, C., Nakafuku, M., Kondoh, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [PubMed] [Article] [RRC of NBRP]
user_report Mikami, Y., GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas. J. Cancer Res. Clin. Oncol. 143 (8): 1381-1393 (2017). PMID 28342001. [PubMed] [Article] [RRC of NBRP]