RDB08066 - pcDNA3.1-His-hGLI3

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pcDNA3.1-His-hGLI3 (#RDB08066)

Expression vector of human Gli3 cDNA

Clone info.	Expression vector of human Gli3 cDNA.
Vector backbone	pcDNA3.1/HisB (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human GLI3 cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08066	pcDNA3.1-His-hGLI3	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-His-hGLI3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08066).

Reference section:

Sasaki, H., Nishizaki, Y., Hui, C., Nakafuku, M., Kondoh, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18391_B0I7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,6X His,T7 tag,Xpress tag,insert 5' Sequence file: RDB18391_B0I7a.seq
>08066_18391_B0I7_3_CMV_Forward_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGCG GTANGCGTGT ACGGNGNGNA GGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACC ATGGGGGGTT 181 CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGG 241 ATCTGTACGA CGATGACGAT AAGGTACCTA AGGATCCAGT GTGGTGGAAT TCTGCAGATG 301 AAGACATCAT GGAGGCCCAG TCCCACAGCT CCACGACCAC TGAAAAGAAA AAAGTTGAGA 361 ATTCCATAGT GAAGTGCTCC ACTCGAACAG ATGTGAGCGA GAAAGCCGTT GCCTCCAGCA 421 CCACTTCTAA TGAGGATGAA AGTCCTGGAC AGACTTATCA CAGAGAGAGA AGAAACGCAA 481 TCACTATGCA GCCACAGAAT GTCCAGGGGC TCAGCAAAGT CAGTGAGGAA CCTTCAACAT 541 CGAGTGACGA GAGGGCCTCA TTGATCAAGA AAGAGATCCA TGGGTCCCTG CCACACGTGG 601 CGGANCCCTC TGTGCCGTAC CGCGGGACGG TGTTTGCCAT GGACCCCAGG AATGGTTACA 661 TGGAGCCCCA CTACCACCCT CCTCATCTTT TCCCTGCCTT CCATCCTCCT GTACCAATTG 721 ATGCCAGACA TCATGANGGC CGTTACCATT ACGATCCATC TCCGATTCCT CCATTGCATA 781 TGACTTCCGC CTTATCTAGT ANCCCTACGT ATCCGGACCT GCCCTTCATT ANGATCTCCC 841 CACACCGGAN NCCNACNGCN GCTTCCNANN CNCCCTTCAG CCCTNCNCAT NCCNACATNA 901 ATCCNTACAT GGNNNATATN NNNNNCNNNC ACANCAGCCN NTCGNNNTNN NNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGHpA,insert 3' Sequence file: RDB18391_B0I7b.seq
>08066_18391_B0I7_3_BGH_rev3_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GNNGGNNNNN NACAGNNGGC TGGCAACTAG AAGGCACAGT 61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCT CAACTCCTAT 121 TGATTTCCGT TGGTTGCAGT CTTTTTTTCC TAAAGCCTAT TGCATAACTG CAAGGAATTT 181 GCTTTCTTCC GCTAGGGAGG TCAGCAAAGA ACTCATGTCC CCGATAGCCA TGTTGGTGGT 241 GCTCATGGAC AGCGCTGGGA ATGGGAGGGA CGCCCGAGGC GTGGTGAGGC GGGAGGAGCT 301 ATGGGAAAGG TTCTGAATGA TACTTGGGCT CAGGGCCCCC GACATCAGGC TGGAGTGGTC 361 CCCATCGTCT ATGATGGCAT CGAAGTCAAT CTGTACCCCT TCCAGGTCAT GGCTGTCGAG 421 GCTGTCCACT GTGCTTGTCA CCTGATTAGC ACCTGGGGAA AGTAACTCAG AGTTTTTGGC 481 GCTGGTCCCC TGTAGCAGGC AGCTGGCGTC TGAAATAGAG AATGAACCAG CTTTCGTGTC 541 TTGCTGACTG AAGCCCACGG TTTGGTCATA GAACTGACCA GAGTAATTCT GCAGATTAGA 601 GCACAGCGGA TGGGGCTGCC CTTTCATCTC CATCTTGATA CCATTCACCC TGCAGGTCTG 661 ACTTGTGTCA CTGAGCTGTC CTGACTGCAG AGCAAGGCTG TCCCTCGGCA TAGCCTGGCG 721 CCTGCTGCCC CCAAAGCTGG CACATGGCTG GTAGCCCCTG ACAACTGCCA AGCTTGACGG 781 CTGGCTGCNC ATGCCGTGAN CCNNNGGCAN GCANCTCTCT GGCCCTTGGT ANATGTTGAT 841 GTGTGANGTA NCACTAATCT GNCNAAGCAT CNGCTNANCG GGGGCNGNCN NNCCCNGGNN 901 NCNGCNNGCT NNNNNNNANN NNNTGGNNAN CNNNNNNNCC NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB18391_B0I7c.seq
>08066_18391_B0I7_3_SV40pro_ori_F_H02_23_ABI24.ab1 1 NNNNNNNNNN NNNNNCCTCT GCCTCTGNNC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG 61 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC 121 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC 181 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT 241 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG 301 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA 361 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 421 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA 481 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC 541 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC 601 TTGTCGATCA GGATGATCTG GACGAANANC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG 661 CCAGGCTCAA GGCGCGCATG CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT 721 GCTTGCCGAA TATCATGGTG GAANATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC 781 TGGGNGTGGC GGNNCGCTAT CANGNNNTAN CGTTGGCTAC CCGNGNTATT GCTGAAGANC 841 TTGGCNGCGA ANNGGCNNNN CGCTTNNNCN NGCTTTACNG NATCGNNGCN NCNNNNTNNN 901 ANNNNATCGN CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [link to RRC of NBRP]
user_report	Patel, R., Novel GLI3 pathogenic variants in complex pre- and postaxial polysyndactyly and Greig cephalopolysyndactyly syndrome Am. J. Med. Genet. A 185 (1): 97-104 (2021). PMID 33058447. [link to RRC of NBRP]

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human GLI3

External Database
Human GLI3

Reference sequence