Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

8xGT-IIC-LucII

Reporter plasmid carring eight copies of Tead-binding motif

Catalog number RDB08067
Resource name 8xGT-IIC-LucII
Clone info. Reporter plasmid carring eight copies of Tead-binding motif.
Comment Monitoring Hippo signaling. See Fig. 1G of Ota and Sasaki, Development 135: 4059-4069 (2008). Use delta51-LucII (cat.# RDB08068) as a negative control.
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank

External Database
firefly (Photinus pyralis) luciferase

            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB08067.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 135, 4059-4069 (2008).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 135, 4059-4069 (2008).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08067 8xGT-IIC-LucII DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The 8xGT-IIC-LucII was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08067).

            Reference section:

            Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Hippo signaling (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19897_B3Bap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Luc-B_R (Pr0790)
            Region: Luc 5',Chicken delta-crystallin Basal pro,8xGT-IIC,SV40 pA
            Sequence file: RDB19897_B3Baa.seq check
            >08067_19897_B3Ba_3_Luc-B_R_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNCTCTTN NTAGCCTTAT GCNGTTGCTC TCCNGCGGTT
               61 CCNTCCTCTA GAGGANAGAA TGGCGCCGGG CCTTTCTTTA TGTTTTTGGC GTCTTCCATT
              121 TTACCAACAG TACCGGAATG CCAAGCTTGG CTGCCCGCAC AGCCCTGCTC ACCGTCTCCG
              181 CAGCTCAGCC CTGGCTGGTC GCTCCGTGCT CTCCGGTCTC ACAGCCCCGC TTTTACGTGC
              241 CCCGGAGCCT GGGCTGGCCC TGCAGGTCGA CGGTATCGAT AAGCTTGATG GACACACATT
              301 CCACAGCTGG GGACACACAT TCCACAGCTG GGGACACACA TTCCACAGCT GGGGACACAC
              361 ATTCCACAGC TGGGGACACA CATTCCACAG CTGGGGACAC ACATTCCACA GCTGGGGACA
              421 CACATTCCAC AGCTGGGGAC ACACATTCCA CAGCATCGAA TTCCTGCAGC CCGGGGGATC
              481 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA
              541 AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA
              601 ATAAACAAGT TAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GANGTGTGGG
              661 ANGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGATAT GGCTGATCCA GACATGATAA
              721 GATACATTGA TGANTTTGGA CAAACCACAA CTAGAATGNA GNGAAAAAAA TGCTTTATTT
              781 GTGAAANTTG TGANGCTATT GCTTTATTTG NAACCNTTAT AAGCTGCAAT AAACNAGTTN
              841 NCNACNNNNG CATNCATTTT ATGNTTCNNN NCNNNGGNNN NNGNNGNNNG NTTTTTTNAN
            901 NCNNGTAAAN NNNNNNNNN
            //
            Primer: Luc_inner_R2 (Pr1286)
            Region: Luc 5',Chicken delta-crystallin Basal pro
            Sequence file: RDB19897_B3Bab.seq check
            >08067_19897_B3Ba_3_Luc_inner_R2_E03_15_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNC TGNNGCANGC AGTTCTATGC GGAAGGGCCA CACCCTTAGG
               61 TAACCCAGTA GATCCAGAGG AATTCATTAT CAGTGCAATT GTTTTGTCAC GATCAAAGGA
              121 CTCTGGTACA AAATCGTATT CATTAAAACC GGGAGGTAGA TGAGATGTGA CGAACGTGTA
              181 CATCGACTGA AATCCCTGGT AATCCGTTTT AGAATCCATG ATAATAATTT TCTGGATTAT
              241 TGGTAATTTT TTTTGCACGT TCAAAATTTT TTGCAACCCC TTTTTGGAAA CAAACACTAC
              301 GGTAGGCTGC GAAATGTTCA TACTGTTGAG CAATTCACGT TCATTATAAA TGTCGTTCGC
              361 GGGCGCAACT GCAACTCCGA TAAATAACGC GCCCAACACC GGCATAAAGA ATTGAAGAGA
              421 GTTTTCACTG CATACGACGA TTCTGTGATT TGTATTCAGC CCATATCGTT TCATAGCTTC
              481 TGCCAACCGA ACGGACATTT CGAAGTATTC CGCGTACGTG ATGTTCACCT CGATATGTGC
              541 ATCTGTAAAA GCAATTGTTC CAGGAACCAG GGCGTATCTC TTCATAGCCT TATGCAGTTG
              601 CTCTCCAGCG GTTCCATCCT CTAGAGGATA GAATGGCGCC GGGCCTTTCT TTATGTTTTT
              661 GGCGTCTTCC ATTTTACCAA CAGTACCGGA ATGCCAAGCT TGGCTGCCCG CACAGCCCTG
              721 CTCACCGTCT CCGCAGCTCA GCCCTGGCTG GTCGCTCCGT GCTCTCCGGT CTCACAGCCC
              781 CGCTTTTACG TGCCCCGGAN CCTGGGCTGG CCCTGCANGT CGACGGTATC GATAAGCTTG
              841 ATGGNNACAC ATTCCACAGC NGGGGANACA CATTCCACAG CNNGGGNNAC ACATTCCACA
              901 NNNNGGGNNN ACACNTTCCN CANCNGGGGN NNNCNCNTNN CACAGCNNGG NNNNACACNN
            961 TNNNNANNNN GGNNNNNNNN NTNNCNNNNN NNNGGNNNNN NNNNN
            //
            Primer: Luc inner_F1 (Pr0617)
            Region: Luc mid
            Sequence file: RDB19897_B3Bac.seq check
            >08067_19897_B3Ba_3_Luc_inner_F1_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTTTG NNNNNGAGTC CTTTGNNCGT GACAAAACAA TTGCACTGAT
               61 AATGAATTCC TCTGGATCTA CTGGGTTACC TAAGGGTGTG GCCCTTCCGC ATAGAACTGC
              121 CTGCGTCAGA TTCTCGCATG CCAGAGATCC TATTTTTGGC AATCAAATCA TTCCGGATAC
              181 TGCGATTTTA AGTGTTGTTC CATTCCATCA CGGTTTTGGA ATGTTTACTA CACTCGGATA
              241 TTTGATATGT GGATTTCGAG TCGTCTTAAT GTATAGATTT GAAGAAGAGC TGTTTTTACG
              301 ATCCCTTCAG GATTACAAAA TTCAAAGTGC GTTGCTAGTA CCAACCCTAT TTTCATTCTT
              361 CGCCAAAAGC ACTCTGATTG ACAAATACGA TTTATCTAAT TTACACGAAA TTGCTTCTGG
              421 GGGCGCACCT CTTTCGAAAG AAGTCGGGGA AGCGGTTGCA AAACGCTTCC ATCTTCCAGG
              481 GATACGACAA GGATATGGGC TCACTGAGAC TACATCAGCT ATTCTGATTA CACCCGAGGG
              541 GGATGATAAA CCGGGCGCGG TCGGTAAAGT TGTTCCATTT TTTGAAGCGA AGGTTGTGGA
              601 TCTGGATACC GGGAAAACGC TGGGCGTTAA TCAGAGAGGC GAATTATGTG TCAGAGGANC
              661 TATGATTATG TCCGGTTATG TAAACAATCC GGAAGCGANC AACGCCTTGN TTGACAAGGN
              721 TGGATGGCTA CNTTCTGGAG ACATAGCTTA CTGGGNCGAA GANGAACACT TCTTCATAGT
              781 TGANNGCTTG AAGTCTTTAA TTNAATACNA AGGANNTCNN NTGGCCCCCG CTGNANTNNG
              841 NANTCGATNT TGTTACNACA CCCCNACATC TTCNANNCNG NNGNNNNNNN TCTTCCNNNN
            901 NANGNNNNNN GNNNNNNNNC NNNNNNNNNN
            //
            Primer: pMAMneo-Reverse (Pr0088)
            Region: Luc 3'
            Sequence file: RDB19897_B3Bad.seq check
            >08067_19897_B3Ba_3_pMAMneo-Reverse_C03_09_ABI24.ab1
                1 NNNNNNNNNN GNNNNNCNNN NNNAGTAAGG NTCCTTCNCN AANNANCCTC TAGAGTATTA
               61 CAATAGCTAA GAATTTCGTC ATCGCTGAAT ACAGTTACAT TTTACAATTT GGACTTTCCG
              121 CCCTTCTTGG CCTTTATGAG GATCTCTCTG ATTTTTCTTG CGTCGAGTTT TCCGGTAAGA
              181 CCTTTCGGTA CTTCGTCCAC AAACACAACT CCTCCGCGCA ACTTTTTCGC GGTTGTTACT
              241 TGACTGGCGA CGTAATCCAC GATCTCTTTT TCCGTCATCG TCTTTCCGTG CTCCAAAACA
              301 ACAACGGCGG CGGGAAGTTC ACCGGCGTCA TCGTCGGGAA GACCTGCCAC GCCCGCGTCG
              361 AAGATGTTGG GGTGTTGTAA CAATATCGAT TCCAATTCAG CGGGGGCCAC CTGATATCCT
              421 TTGTATTTAA TTAAAGACTT CAAGCGGTCA ACTATGAAGA AGTGTTCGTC TTCGTCCCAG
              481 TAAGCTATGT CTCCAGAATG TAGCCATCCA TCCTTGTCAA TCAAGGCGTT GGTCGCTTCC
              541 GGATTGTTTA CATAACCGGA CATAATCATA GGTCCTCTGA CACATAATTC GCCTCTCTGA
              601 TTAACGCCCA GCGTTTTCCC GGTATCCAGA TCCACAACCT TCGCTTCAAA AAATGGAACA
              661 ACTTTACCGA CCGCGCCCGG TTTATCATCC CCCTCGGGTG TAATCAGAAT AGCTGANGTA
              721 GTCTCAGTGA GCCCATATCC TTGTCGNATC CCTGGAAGAN GGAAGCGTTT TGCAACCGCT
              781 TCCCCGANTT CTTTCGAAAG ANGTGCGCCC CCNGAAGCAA TTTCGTGNNA ATTAGANNAA
              841 TCGTATTTGN CNNNCANANN GCTTTTGGNN AANAATGNAA ATNGGGNNGG NACTANCNNC
            901 GCACTTNNNA NTTTNNNAAN NCNNGNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856.
            reference Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852.
            reference Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl