Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK106

Expression clone for an auxin-based degron (AID) system, aid-CENPH.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18378_B0Hhp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,OsTIR1 5' Sequence file: RDB18378_B0Hha.seq
>08469_18378_B0Hh_3_CMV_Forward_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNTNNN GCNNATGGGC GGTANGCGTG TACGGTGGGN NGTCTATATA    61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGA   121 TGACGTACTT CCCGGAGGAG GTGGTGGAGC ACATCTTCAG CTTCCTGCCG GCGCAGCGCG   181 ACCGCAACAC GGTCTCGCTC GTCTGCAAGG TGTGGTACGA GATCGAGAGG CTGAGCCGCC   241 GCGGCGTCTT CGTGGGCAAC TGCTACGCCG TGCGCGCCGG CCGCGTCGCC GCGCGGTTCC   301 CCAACGTGCG GGCGCTCACG GTGAAGGGGA AGCCCCACTT CGCCGACTTC AACCTCGTGC   361 CCCCCGACTG GGGCGGCTAC GCGGGGCCGT GGATCGAGGC GGCCGCGAGG GGATGCCACG   421 GCCTGGAGGA GCTCAGGATG AAGCGGATGG TGGTGTCCGA CGAGAGCCTC GAGCTGCTGG   481 CTCGCTCGTT CCCGCGGTTC AGGGCTCTTG TTCTTATCAG CTGCGAGGGG TTCAGCACTG   541 ACGGGCTAGC CGCCGTCGCG AGCCATTGCA AGCTTCTGAG GGAGTTGGAT TTGCAGGAAA   601 ATGAAGTGGA GGATCGAGGG CCTAGGTGGC TTTCCTGCTT CCCTGATTCC TGCACATCAC   661 TTGTCTCATT GAATTTTGCC TGCATCAAAG GGGANGTTAA TGCTGGTTCA CTGGAGAGAC   721 TTGTTAGCAG GTCCNCAAAC CTGCGGANTT TGANGCTGAN TCGATCTGTA TCGGTAGATA   781 CACTTGCNAA GATACTACTG CGTACNNCTA ACTTGNNNGA NTTGGGGANA NGGAATTTGN   841 CAGATGANTT CCNAACTGAN TCCTANTTTN AGCTTACCAN NNCTCTGGAN AANTGNNANA 901 NGTTGANGAN TTTGTCNGGN NTCTGGNNNN NNTCNNCNNG TTNNNNNNNN NN //
Primer: IRES-R (Pr0037) Region: 9XMyc,OaTIR1 3' Sequence file: RDB18378_B0Hhb.seq
>08469_18378_B0Hh_3_IRES-R_C11_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTTA NGGGGGGGGG NNGGNNNNGG GCGGNTCCCG    61 GGGNNCTCTT GAATGATCGT TCCACTTTTT AGCTAGTGGA TCCGTTCAAG TCTTCTTCTG   121 AGATTAATTT TTGTTCACCG TTCAAGTCTT CCTCGGAGAT TAGCTTTTGT TCACCGTTCA   181 AATCTTCTTC AGAAATCAAC TTTTGTTCAC CTCTAGAGGA TCCGTTCAAG TCTTCTTCTG   241 AGATTAATTT TTGTTCACCG TTCAAGTCTT CCTCGGAGAT TAGCTTTTGT TCACCGTTCA   301 AATCTTCTTC AGAAATCAAC TTTTGTTCAC CTCTAGAGGA TCCGTTCAAG TCTTCTTCTG   361 AGATTAATTT TTGTTCACCG TTCAAGTCTT CCTCGGAGAT TAGCTTTTGT TCACCGTTCA   421 AATCTTCTTC AGAAATCAAC TTTTGTTCAC CACTAGCAGC AGAACCGGAG TCGACTAGGA   481 TTTTAACAAA ATTTGGTGCA TCATCCCTCG CCCCAGCAGT TGTGCGGTAC ACATATAATT   541 TCTCCACTTT AGGCAGATCT CCATGGTTTT CCTCCATTTC ATTGCTACCA TCCCGCTCAT   601 TTATGACCTC AACATTGAGC ATCGGCATCT TTGACGCAAG GACTTGGCAC CCCTTTAACG   661 TGACATTGCA AGATGACATC CAAAGGGATC GCATTGTCTC GTACCTAGCA AAATTCCCCA   721 AGAGTGCAGC ATCACCAAAC GGGCTATCTC TTATCTCCNG TTTCCTTAAA TTCTTGCATC   781 CATTCATAAC ATGCATCATA CCCTTATCAC TGTCACCAGC AAATGCTATA NAAAGCATCT   841 CNAGTTGTTT TGCATATTTC CNAATATACA TGAAAACTTT GTCTGTGAGA NNANCAGATA   901 TTGANAAACG TTNNNATCCC NNGCACNCNC NAACNNNANN NNCNAANNNT TNNTCNNATG   961 GTNNNNTTGN CNCNNNNTCN GNNTNNCNNG GNNNNNNNAN NNNNANNNCN GNANNNNNNN 1021 NNN //
Primer: IRES-F (Pr0036) Region: IRES2,AID 5' Sequence file: RDB18378_B0Hhc.seq
>08469_18378_B0Hh_3_IRES-F_D11_11_ABI24.ab1     1 NNNNNNNNNN NNNNTNNNTC GNNGTTNNNA AACGTCTAGG CCCCCCGAAC CNCGGGGNCG    61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGGCAG TGTCGAGCTG   121 AATCTGAGGG AGACTGAGCT GTGTCTTGGT CTTCCCGGTG GAGATACAGT GGCTCCGGTA   181 ACCGGAAACA AGAGAGGGTT CTCAGAGACG GTTGATCTGA AGCTAAATCT GAATAATGAG   241 CCTGCAAACA AGGAAGGATC TACGACTCAT GACGTCGTGA CTTTTGATTC CAAGGAGAAG   301 AGTGCTTGTC CTAAAGATCC AGCCAAACCT CCGGCCAAGG CACAAGTTGT GGGATGGCCA   361 CCGGTGAGAT CATACCGGAA GAACGTGATG GTTTCCTGCC AAAAATCAAG CGGTGGCCCG   421 GAGGCGGCGG CGTTCGTGAA GGTATCAATG GACGGAGCAC CGTACTTGAG GAAAATCGAT   481 TTGAGGATGT ATAAAAGCTA CGATGAGCTT TCTAATGCTT TGTCCAACAT GTTCAGCTCT   541 TTTACCATGG GCAAACATGG AGGAGAAGAA GGAATGATAG ACTTCATGAA TGAGAGGAAA   601 TTGATGGATT TGGTGAATAG CTGGGACTAT GTTCCCTCTT ATGAAGACAA AGACGGTGAT   661 TGGATGCTCG TCGGCGACGT TCCTTGGCCA ATGTTCGTCG ATACATGCAA GCGTTTACGT   721 CTCATGAAAG GATCGGATGC CATTGGTCTC GCTCCGAGGG CGATGGAGAA GTGCAAGANC   781 AGANCTGCGG CCGGANCTGG TGCANGCGCT GGANCGGGTG CCGATATCGA ATTCACCANG   841 GCGGGGCGGC TGTCGGANNN CGTCNGGNNC NGGCCGGGCG CTNANGCCNA NACNGCGGCC 901 NATNCNNNNG CNAANCGGGN NNNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: bGHpA,chiken CENP-H 3' Sequence file: RDB18378_B0Hhd.seq
>08469_18378_B0Hh_3_SV40polyA_R2_B11_05_ABI24.ab1     1 NNNNNNNNCN NNNNNNNNNN NGTTNNNAAC AACAATTGCA TTCNTTTTAT GTTTCAGGTT    61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT   121 GATTATGATC TAGAGTCGCG GCCGCAGTGA TCACAGTACT CTACAAGAGA TTCTGAATGG   181 AGATATCTTT CTCGAGCTGT AGAATAATTG CTCTTAAAGA TGGATCTTTC GCCCAGTTGA   241 TTTGGCATCC GACTATGATA TTCTGGAGTA TATTTTGAAC TAACGTAGTC ACCTTCCTTT   301 CATACTGTAC ATTCCTATGG ATAAACTTCA ACTTTTCAGC AATTTTCATT CTGACTACTT   361 CCTTTTTTTT CCTCACTGTG GTAAAAATTC GCAGCGCCTT CTCTCCTTCG GCCTGTTTCA   421 GAAAGTATCT TTTCCTTCTA ATGTCATTCA AATTCTCTCT AATCACACGT GCTTGCTGAT   481 GAGCTTGAAT TATTTCCCAG TTGAGCTTGA TTATGTCTCT CATTGTTTCC ACGATCAGTC   541 GTGCGCAACC ATCATTTTCT TTCACTCTCT TTCTCAGAGC ATCCATAAGC TGGATCCTTT   601 TTATGACTAA TGCTTTGCTT TCAAAGGCAC TTTTTACTTT CCCCACTTCT TCCAAAGTTT   661 CTTTGGCACT TTCGATAAAG TTTACTTCCG CGCCACTGTT GCACCCTTCA TCGGGGGGGC   721 ACGCGGTGTC GAACTCCATC ACCAGCTGCT TCAGATGCGT CCTCGCGCAN ANGTGCTCCA   781 GGACGTCCCG CTTCGCGTCC GGNTCGGCCG CCGTCTCGGC CTCAGCGCCC GGCCCGGANC   841 CNANGGNNTC CGACAGCCGC CCCGCCANGG NGAANTNNAT ATCGGCACCN GCNCCAGCGC   901 NNGNNCCANC TCCNGNCGCA NCNNNGCNNN NGCNNTTNNN CNTCNNCCTC NNNNCNNNNN 961 NNNNGNNNNN NNNNN //
Primer: CENP-M_R (Pr0887) Region: chiken CENP-H 5',AID 3' Sequence file: RDB18378_B0Hhe.seq
>08469_18378_B0Hh_3_CENP-M_R_E11_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCNCNN GCTGCTTNNG ATGCGTCCTC GCGCAGANGT    61 GCTCCAGGAC GTCCCGCTTC GCGTCCGGAT CGGCCGCCGT CTCGGCCTCA GCGCCCGGCC   121 CGGACCCGAC GGACTCCGAC AGCCGCCCCG CCATGGTGAA TTCGATATCG GCACCCGCTC   181 CAGCGCCTGC ACCAGCTCCG GCCGCAGCTC TGCTCTTGCA CTTCTCCATC GCCCTCGGAG   241 CGAGACCAAT GGCATCCGAT CCTTTCATGA GACGTAAACG CTTGCATGTA TCGACGAACA   301 TTGGCCAAGG AACGTCGCCG ACGAGCATCC AATCACCGTC TTTGTCTTCA TAAGAGGGAA   361 CATAGTCCCA GCTATTCACC AAATCCATCA ATTTCCTCTC ATTCATGAAG TCTATCATTC   421 CTTCTTCTCC TCCATGTTTG CCCATGGTAA AAGAGCTGAA CATGTTGGAC AAAGCATTAG   481 AAAGCTCATC GTAGCTTTTA TACATCCTCA AATCGATTTT CCTCAAGTAC GGTGCTCCGT   541 CCATTGATAC CTTCACGAAC GCCGCCGCCT CCGGGCCACC GCTTGATTTT TGGCAGGAAA   601 CCATCACGTT CTTCCGGTAT GATCTCACCG GTGGCCATCC CACAACTTGT GCCTTGGCCG   661 GAGGTTTGGC TGGATCTTTA GGACAAGCAC TCTTCTCCTT GGAATCAAAA GTCACGACGT   721 CATGAGTCGT AGATCCTTCC TTGTTTGCAG GCTCATTATT CAGATTTAGC TTCAGATCAA   781 CCGTCTCTGA NAACCCTCTC TTGTTTCCGG TTACCGGANC CACTGTATCT CCACCGGGAA   841 NANCAAGANA CAGCTCANTC TCCNTCANAN TCAGCTCNAC ACNGCCCANG GNTGNGGNCN   901 TATNATCATC NNGNTTTTTC NAANGNAAAN CACNNNCCCN NNNNNTNCNG GGGGGNCNNN 961 NNNNNTTTTT TNNNNCNNNN NNNNANNNNN NNNNANNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB18378_B0Hhf.seq
>08469_18378_B0Hh_3_SV40pro_ori_F_F11_17_ABI24.ab1     1 NNNNNNNNNN NNNNGNNGCC TCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT    61 TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG   121 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC   181 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG   241 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA   301 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC   361 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT   421 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG   481 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC   541 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG   601 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC   661 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC   721 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA TCANGACATA   781 GCGTTGGCTA CNNGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA CCGCTTCCTC   841 GNGCTTTACN GNATCGCCGC TCCCNANTCN CANCGCATCG CCTTCNATCG CNNTCNNGAN 901 NANNNNNTCN NNNCGGNNNN NNNGGNNNNC NAAANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

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