Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMT-PURO

Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.

Sequence (full)	RDB08532zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology 57(1), 45-49, 2008).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cytotechnology 57(1), 45-49, 2008)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08532_A2Gap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: MT pro, BiP signal sequence Sequence file: RDB08532_A2Gaa.seq
>08532_08532_B1CtF_HB-NOS_R_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT TGGGTNNNGC CNGGGTTTTC CCNGTCACGA CGTTGTAAAA    61 CGACGGCCAG TGAATTAATT CGTTGCAGGA CAGGATGTGG TGCCCGATGT GACTAGCTCT   121 TTGCTGCAGG CCGTCCTATC CTCTGGTTCC GATAAGAGAC CCAGAACTCC GGCCCCCCAC   181 CGCCCACCGC CACCCCCATA CATATGTGGT ACGCAAGTAA GAGTGCCTGC GCATGCCCCA   241 TGTGCCCCAC CAAGAGCTTT GCATCCCATA CAAGTCCCCA AAGTGGAGAA CCGAACCAAT   301 TCTTCGCGGG CAGAACAAAA GCTTCTGCAC ACGTCTCCAC TCGAATTTGG AGCCGGCCGG   361 CGTGTGCAAA AGAGGTGAAT CGAACGAAAG ACCCGTGTGT AAAGCCGCGT TTCCAAAATG   421 TATAAAACCG AGAGCATCTG GCCAATGTGC ATCAGTTGTG GTCAGCAGCA AAATCAAGTG   481 AATCATCTCA GTGCAACTAA AGGGGGGATC CGATCTCAAT ATGAAGTTAT GCATATTACT   541 GGCCGTCGTG GCCTTTGTTG GCCTCTCGCT CGGGAGANCT TCGCGAACTA GTCCGGANAT   601 CCACGTGTGT ACACCTANGC TTAAGTCTAG AGGGCCNTTC NAANGNAAGC CTATCCCTAA   661 CCCTCTCCTC NGTCTCGANT CNACNCNNAN CNGTCATCAT CNNNATCNCN ANNNNNTTNN 721 ANNCNNNNNN NNNN //
Primer: reverse (Pr0099) Region: SV40 pA, 6xHis, V5 tag, BiP signal sequence, MT pro Sequence file: RDB08532_A2Gab.seq
>08532_08532_B1CtF_reverse_F02_17_ABI24.ab1     1 NNNNNNNNNN NNCNNNNNNN NNTAATCNGC CNTACCACAT TTGTAGAGGT TTTACTTGCT    61 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT   121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC   181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA   241 TCTTATCATG TCTGGATCCT CGATCGAGGC TGATCAGCGA GCTCAGGCCT TAGAAGGCAC   301 AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG TGATGATGAC CGGTACGCGT   361 AGAATCGAGA CCGAGGAGAG GGTTAGGGAT AGGCTTACCT TCGAAGGGCC CTCTAGACTT   421 AAGCCTAGGT GTACACACGT GGATATCCGG ACTAGTTCGC GAAGATCTCC CGAGCGAGAG   481 GCCAACAAAG GCCACGACGG CCAGTAATAT GCATAACTTC ATATTGAGAT CGGATCCCCC   541 CTTTAGTTGC ACTGAGATGA TTCACTTGAT TTTGCTGCTG ACCACAACTG ATGCACATTG   601 GCCAGATGCT CTCGGTTTTA TACATTTTGG AAACNCGGCT TTACACACGG GTCTTNCGTT 661 CNANTNNCCT CNTTNNNANN CNNNNGNNNN GCTNNNAANT NNNNNNNNNN //
Primer: COPIApro-F (Pr0259) Region: copia pro, PuroR Sequence file: RDB08532_A2Gac.seq
>08532_08532_B1CtF_COPIApro-F_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCTT TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG GTCGTGGTGC    61 TGGTGTTGCA GTTGAAATAA CTTAAAATAT AAATCATAAA ACTCAAACAT AAACTTGACT   121 ATTTATTTAT TTATTAAGAA AGGAAATATA AATTATAAAT TACAACAGAT CCCCCGGGCT   181 GCAGGAATTC GATAGCTTGC ATGCCTGCAG GTCGGCCGCC ACGACCGGTG CCGCCACCAT   241 CCCCTGACCC ACGCCCCTGA CCCCTCACAA GGAGACGACC TTCCATGACC GAGTACAAGC   301 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG   361 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG   421 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG   481 TCNNGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC NGANAGCGTC GAAGCGGGGG   541 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGANTTGAG CGGNTCCNGG CTGGNCGCGC   601 ANCAACAGAN GGNANGGNCT CCTGGCGCCN CANCNGCCNN ANGNANCCCN NGNNGNNNCN 661 NGNNNNNNGN NNNNNTCNNN NNNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, SV40 pA Sequence file: RDB08532_A2Gad.seq
>08532_08532_B1CtF_Amp_R_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNTNANCAT TTATNNGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA    61 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT   121 GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG   181 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG   241 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG   301 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA   361 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC   421 ATCAGGCGCC GGTACCCGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA   481 CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT   541 TTGTAACCAT TATAAGCTGC AATAAACAAG TTCAAGCTAG CTGGCCTCGA NGTCGACGGT   601 ATCGATAAGC TTGATCTAGA GTCNGTGGGC CTCGGGGGCG GGNGCGGGGG NCGGCGGGGC   661 CGCCNNNGGN NGNTTNNNNC NNNNCCNTGG GGNCNNGNGC TNNNTTNNGN NNNGGNNNNN 721 NNGNNNNNNG GGGNNNGGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl