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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGFPS1

Plasmid clone of GFPS1, a GFP mutation variant

Catalog number RDB09057
Resource name pGFPS1
Alternative name GFP mutation variant
Clone info. GFPS1 gene with a T7 promoter and terminator. GFPS1 is a GFP mutation variant, with F46L, S65T, F99S, M153T, V163A, Y200C, to enhance the brightness and the maturation speed of GFP. Fluorescence can be detected by a fluorescence plate reader with excitation at 485 nm and emission at 507 nm (Seki, E., Anal. Biochem. 377 (2) 156-161, 2008).
Vector backbone pCR2.1-TOPO (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r, Kan^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kigawa, Takanori |
Other clones in our bank
External Database
Aequorea victoria GFP
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR is requested (Anal.Biochem.377,156-161(2008)). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the BIOLOGICAL RESOURCE, or the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT must contact the DEPOSITOR in the case of transfer, sell, or assign the BIOLOGICAL RESOURCE, or the products derived from the BIOLOGICAL RESOURCE, to any third party.
            Additional terms and conditions:
            The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
            Remarks The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用する (Anal.Biochem.377,156-161(2008))。利用者が本件リソース及び本件リソースを使用して得られた研究成果に基づき特許等の申請、又は事業活動を行う場合は、寄託者と別途協議を行う。利用者が本件リソース及び本件リソースに由来する産物を第三者へ移転、譲渡または分与する場合は、寄託者と別途協議を行う。
            付加的提供条件：
            非営利機関の非営利学術研究に限って提供する。
            備考 非営利機関の非営利学術研究に限って提供する。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB09057 pGFPS1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pGFPS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09057).

            Reference section:

            Seki, E., Matsuda, N., Yokoyama, S., Kigawa, T., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09057_B2F1p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100 (Pr0310)
            Region: T7 pro.1,T7 pro.2,insert 5'
            Sequence file: RDB09057_B2F1a.seq check
            >09057_09057_B2F1_3_pUC-M13_-100_B08_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC CNGGGTTTTC CCNGTCACGA CGTTGTAAAA
               61 CGACGGCCAG TGAATTGNNN NNNNACTCAC TATAGGGCGA ATTGGGCCCT CTAGATGCAT
              121 GCTCGAGCGG CCGCCAGTGT GATGGATATC TGCAGAATTC GCCCTTGCTC TTGTCATTGT
              181 GCTTCGCATG ATTACGAATT CAGATCTCGA TCCCGCGAAA TTAATACGAC TCACTATAGG
              241 GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA
              301 TATGAAAAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA
              361 TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA
              421 CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGCCAAC
              481 ACTTGTCACT ACTTTGACTT ATGGTGTTCA ATGCTTTTCC CGTTATCCGG ATCACATGAA
              541 ACGGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAC GCACTATATC
              601 TTTCAAAGAT GACGGGAACT ACAAGACGCG TGCTGAAGTC AAGTTTGAAG GTGATACCCT
              661 TGTTAATCGT ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTCGGACA
              721 CAAACTCGAG TACAACTATA ACTCACACAA TGTATACATC ACGGCAGACA AACAAAAAGA
              781 NNGGAATCAA AGCTAACTTC NAAATTCNNC ACAACATTGN AAGANGGTTC CGTTCAACTA
              841 GCAGANCATT ATCAACNAAA TACTCCNANT TGGCGNNGGN CCTGTNCTTT TANCAGACAN
              901 NCATTGNNNG TCNACNNAAT CTGCCNTTNC NAANNATCCC NACGAAAAAG CGTGANCACN
              961 NNGNNNNTTN NTGNNTTNGN AACTGNTGCT GGGNNTNCNC NTNGNNNNGN NTNANCNCNA
            1021 NNANNAANGA ANTANNTNCN ANNNGANCNN NNNNNGNNNG NNNNNNNNNN
            //
            Primer: reverse-2 (Pr0100)
            Region: T7 ter,insert 3'
            Sequence file: RDB09057_B2F1b.seq check
            >09057_09057_B2F1_3_reverse-2_A08_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCTTGGTANC GAGCTCGGNN CCNCTAGTAA CGGCCGCCAG
               61 TGTGCTGGAA TTCGCCCTTG CTCTTGTCAT TGTGCTTCGT ATAAAAATAG GCGTATCACG
              121 AGGCCCTTTC GTCTTCAAGA ATTATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT
              181 CAAGACCCGT TTAGAGGCCC CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC
              241 CAACTCAGCT TCCTTTCGGG CTTTGTTAGC AGCCGGATCT GCTCGAGCGG CCGCCAGTGT
              301 GATGGATTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC AGCTTCCTTT CGGGCTTTGT
              361 TTAGCAGCCT AGGTATTAAT CAATTAGTGG TGGTGGTGGT GGTGGTGGTG CTCGAGTGCG
              421 GCCGCAAGCT TGCTAGCGGC GCTCAGTTGG AATTAATTCA TTATTTGTAG AGCTCATCCA
              481 TGCCATGTGT AATCCCAGCA GCAGTTACAA ACTCAAGAAG GACCATGTGG TCACGCTTTT
              541 CGTTGGGATC TTTCGAAAGG GCAGATTGTG TCGACAGGCA ATGGTTGTCT GGTAAAAGGA
              601 CAGGGCCATC GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA ACGGAACCAT
              661 CTTCAATGTT GTGGCGAATT TTGAAGTTAG CTTTGATTCC ATTCTTTTGT TTGTCTGCCG
              721 TGATGTATAC ATTGTGTGAG TTATAGTTGT ACTCGAGTTT GTGTCCGAGA ATGTTTCCAT
              781 CTTCTTNNAA ATCAATACCN TTTAACTCGA TACGATTAAC AAGGGTATCA CCTTCAAACT
              841 TGACTTCAGC ACGCGTCTTG NAGTTNCCGT CATCTTTGAA ANANATANTG CGTTCCTGTA
              901 CATAACCTTC NGGGCATGNN ACTCTTGAAA AAGTCNTGCC GTTTCATGTG ATCCGGANAA
              961 CGGGAAAGCN TTNNAACACC ATAAGTNAAA GTANTGNNNN NNGNTNGNNN NNNNNANNNN
            1021 GNNANNTNNN NNNNNNN
            //
            Primer: phBG_F (Pr0916)
            Region: KanR
            Sequence file: RDB09057_B2F1c.seq check
            >09057_09057_B2F1_phBG_F_E03_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTTGNCNN NNNNNNCTGN NGGCGCAGGG GNNCAAGATC
               61 TGATCANNGA GACAGGATGA NGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
              121 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
              181 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
              241 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
              301 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
              361 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCCCAC CTTGCTCCTG
              421 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
              481 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
              541 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
              601 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
              661 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NNNNACTGTG
              721 GCCGGCTGGG TGTGGCGGAC CGCTATCNGG ACATAGCGTT GGCTACCCGT GATATTGCTG
              781 ANNAGCTTGG CGGCNAATGG GCTGACCGCT TCCTCGTGCT TTANNGTATC NCCGCTCNNG
              841 ATTCNCAGCG CATCGCCTTC NNNNNNNNTC TTGACGAGNT NCTTCTGANN NNAANANNGA
              901 AGANTATGAG TATTCANNAT TTCCGTGTCG CCCNTNNNNN CCTTTTTNNG GCATTTTGCC
              961 TTNNNNNTTT TGNTCNCCNN NAAANNGCNG GNNGAAAGTA AAAGANGCTG AAANNNNNNN
             1021 TNNGNNNNNN CNANTGGGNN NNNTNNAACT NGNANNNTCN NNNNCNGNNN NNNNNNCNTN
            1081 NNNNTTTNNN NNCCNANNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Seki, E., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196.

            2023.05.01

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