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pMYs-Tead2-VP16-IRES-EGFP

Retroviral vector of mouse Tead2, constitutive active

Catalog number RDB12174
Resource name pMYs-Tead2-VP16-IRES-EGFP
Clone info. Retroviral vector of mouse Tead2, activation domain of herpes simplex virus VP16, constitutive active. cDNA fragment 87 to 1,424 nt of NM_011565.2 (CDS, 87..1424) is cloned.
Comment Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to A substitution at 453 nt (Val to Ile at 123rd aa).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.2 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA HSV VP16 protein cDNA
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank

External Database
Mouse Tead2 HSV VP16 protein

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infections virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          付加的提供条件:
          pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12174 pMYs-Tead2-VP16-IRES-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMYs-Tead2-VP16-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12174).

          Reference section:

          Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19899_B3Bap1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F (Pr0352)
          Region: pol region,insert 5'(Tead2 1-127aa)
          Sequence file: RDB19899_B3Baa.seq check
          >12174_19899_B3Ba_1_MMLV-pol_F_B05_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG GCNTCGNAGC TTGGNNNCNC GCCGCCCACG TGAAGGCTGC
             61 CGACCCCGGG GGTGGACCAT CCTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCC
            121 AGTGTGGTGG TACGGGAATT CCTGCAGGAG CTATTTAGGT GACACTATAG AATACAAGCT
            181 ACTTGTTCTT TTTGCAGGAT CCCATCGATT CGAATTCGAG GCCCAGATGG GGGATCCCCG
            241 GACTGGGGCC CCCCTGGATG ATGGCGGGGG CTGGACAGGT AGCGAGGAAG GCAGCGAAGA
            301 GGGCACCGGA GGCAGCGAGG GCGTTGGAGG TGATGGCAGC CCTGATGCAG AAGGTGTGTG
            361 GAGCCCCGAC ATTGAGCAGA GTTTTCAGGA GGCCCTGGCC ATCTACCCTC CCTGTGGCCG
            421 TCGCAAGATC ATCCTGTCAG ATGAGGGCAA GATGTATGGC CGGAACGAAC TCATTGCCCG
            481 TTACATCAAG CTGAGGACAG GGAAGACGAG AACGCGAAAG CAGGTCTCCA GCCATATTCA
            541 GGTTTTGGCT CGAAGGAAAT CGAGAGAAAT TCAGTCCAAG CTGAAGGACC AAATCTCCAA
            601 GGACAAGGAN TTCCCGGGGA TCTGGGCCCC CCCGACCGAT GTCAGCCTGG GGGACGAGCT
            661 CCACTTAGAC GGCGAGGACG TGGCGATGGC GCATGCCGAC GCGCTAGACG ATTTCGATCT
            721 GGACNTGTTG GGGGANGGNN NTTCCCCGGN NCCGGGATTT ACNNNNCACG ACTCCGCCCC
            781 CTACNGCGCT CTGGATATGG NCNANTNCNN NTTTGANCAG ATGTTTACCG ATGCCNNNNN
            841 NANNTGACNA NNACNGNGGG NANGNNTCGA GGNNCNGNNN NNNNNNGNNN NCTACNNNNN
          901 TNNNNNNNNN N
          //
          Primer: Tead2-B_R (Pr1297)
          Region: insert 3'(Tead2 1-127aa 3',VP16 AD),IRES
          Sequence file: RDB19899_B3Bab.seq check
          >12174_19899_B3Ba_1_Tead2-B_R_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCNGC NNTATTNNGG TTTTGGCTCG AAGGAAATCG AGAGAAATTC
             61 AGTCCAAGCT GAAGGACCAA ATCTCCAAGG ACAAGGAATT CCCGGGGATC TGGGCCCCCC
            121 CGACCGATGT CAGCCTGGGG GACGAGCTCC ACTTAGACGG CGAGGACGTG GCGATGGCGC
            181 ATGCCGACGC GCTAGACGAT TTCGATCTGG ACATGTTGGG GGACGGGGAT TCCCCGGGTC
            241 CGGGATTTAC CCCCCACGAC TCCGCCCCCT ACGGCGCTCT GGATATGGCC GACTTCGAGT
            301 TTGAGCAGAT GTTTACCGAT GCCCTTGGAA TTGACGAGTA CGGTGGGTAG GCCTCGAGGG
            361 CCGGCGCGCC GCGGCCGCTA CGTAAATTCC GCCCCCCCCC CCCCTCTCCC TCCCCCCCCC
            421 CTAACGTTAC TGGCCGAAGC CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC TATATGTTAT
            481 TTTCCACCAT ATTGCCGTCT TTTGGCAATG TGAGGGCCCG GAAACCTGGC CCTGTCTTCT
            541 TGACGAGCAT TCCTAGGGGT CTTTCCCCTC TCGCCAAAGG AATGCAAGGT CTGTTGAATG
            601 TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT CTTGAAGACA AACAACGTCT GTAGCGACCC
            661 TTTGCAGGCA GCGGAACCCC CCACCTGGCG ACAGGTGCCT CTGCGGCCAA AAGCCACGTG
            721 TATAAGATAC ACCTGCNAAG GCGGCACAAC CCCAGTGCCA CGTTGTGAGT TGGATAGTTG
            781 TGGAAAGAGT CAAATGGCTC TNCTCNAGCG TATTCAACAN NGGGCTGAAN GATGNCCANA
            841 ANGNNNCCCA TTGNATNGGA TCTGATCTGG GGNCTCNGNG CNCNTGCTTT NCNTGTGTTT
            901 AGTCNANGNT NAAAAAACGT CTAGGCCCCC CNNANCNNNG GGNNGNGNNT TNNCTTNNAA
          961 AAANNNNANG ANNNNANNGN NNNNNNNNN
          //
          Primer: IRES-F (Pr0036)
          Region: IRES,EGFP 5'
          Sequence file: RDB19899_B3Bac.seq check
          >12174_19899_B3Ba_1_IRES-F_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG
             61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
            121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
            181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
            241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
            301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
            361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
            421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
            481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
            541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
            601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
            661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCNCC
            721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
            781 GTGANCGCCG CCGGNATCAC TCTCGGNNTG GANNANCTGT ACAAGTAAAG CGGNNGGNNG
            841 CCAGCACAGN GGTCGANCGG NNCNCCNGCN CNNNGGNCNA TCGANGGGGG GNCCGCGANN
            901 NNTCCNATTT GNNAAAGANN GGATATCNNN NNNNCNNNNT CNNNNTTNNN TCNNCNNNNT
          961 NNNNNGNTNN NNNNNNNN
          //
          Primer: Amp_R (Pr0008)
          Region: AmpR pro,5'LTR
          Sequence file: RDB19899_B3Bad.seq check
          >12174_19899_B3Ba_1_Amp_R_F05_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTTG NNGCNTTTAT NNGGGTTATT GTCTCATGAG CGGATACATA
             61 TTTGAATGTA TTTAGAAAAA NNNNNNNATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
            121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
            181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
            241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
            301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
            361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
            421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
            481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
            541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
            601 GAGTCGCCCG GGTACCCGTA TTCCCNATAA AGCCTCTTGC TGTTTGCATC CGAATCGTGG
            661 ACTCGCTGAT CCTTGNNAGG GTCTCCTCAG ATTGATTGAC TGCNNACCTC GGGGGNCTTT
            721 CATTTGNAGG NTCNACCGAN ATTNGGAGAN CCCTGCCNNG GGNNNNCNGA CCCCNCNNNN
            781 NGGAGGNANG CNNGNCNNGC GNNCGNTTCN NGNCTGNNNN TGNCNTNGNN NGNGNTNNNG
          841 NNNNNNTNNN NNNNTNNNNN CNGNGNCNGN NCNANTNAGN TNNNTANNNN NNNNNN
          //
          Primer: pMFG_1 (Pr0230)
          Region: pol region,psi
          Sequence file: RDB19899_B3Bae.seq check
          >12174_19899_B3Ba_1_pMFG_1_D05_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNC TTGTNNTGTC AGGGTCAGGG NAGTTTACAA
             61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
            121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
            181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
            241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
            301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
            361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
            421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
            481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CTAATTTTCA GACAAATACA
            541 GAAACACAGT CAGACAGAGA CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC
            601 GGCTTCGGTC CCAAACCGAA AGCAAAAATT CAGACGGAGG CGGGAACTGT TTTAGGTTCT
            661 CGTCTCCTAC CAGAACCACA TATCCCTCCT ATTAGGGGGG TGCACCAAAG AGTCCAAAAC
            721 GATCGGGATT TTTGGANTCN NGTCGGGCCN CAAAAACGGC CCCCGAAGTC CCTGGGNNGT
            781 CTCCCAGGGT TGCGGNCGGG TGTTCCNAAC TCGTCAGTTC CACCACGGGT CCGCCNGANA
            841 CAGANCTNGT TAGCTAACTA GTACAGACGC NNNGCAAACN TTANATGCCN GNNCNAACNC
          901 NCACNAAGNC NNANNNNNAN NCNAAACNAN NNCNNGNNNN NNNTNNNNNN NNNN
          //
          Primer: mVenus-M_F (Pr0574)
          Region: EGFP 3',3'LTR
          Sequence file: RDB19899_B3Baf.seq check
          >12174_19899_B3Ba_1_mVenus-M_F_G05_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGCN TCNAGGTGAN TTNNAGANCC GCCACAACAT CGAGGANGGC
             61 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
            121 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG
            181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
            241 GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT CGACCGGGCC GCCAGCACAG
            301 TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT AAAGACAGGA TATCAGTGGT
            361 CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG CCTATAGAGT ACGAGCCATA
            421 GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG
            481 TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA TACATAACTG
            541 AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC AGCAGAATAT GGGCCAAACA
            601 GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCANGGCC AAGAACAGTT GGAACAGCAG
            661 ANTATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCN NGGCCAAGAA
            721 CAGATGGNCC CCANATGCGG NCCCGCCCTC ANCAGTTTCT ANAGAANCAT CANATGTTTN
            781 NNGGGNGNCC NNNNNNNCTG AANTGACCCT NNNNNNNANT TGAANTNANC NATCANNNCG
          841 NTTCNCNCNN NNNNNCNNGN NNNNNNGNNN CNNNANNNNN NNAAAANANN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.
          user_report Zhang, J., TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma. Am. J. Pathol. pii: S0002-9440 (18) 30776-4 (2019). PMID 30794805.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl