Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pRSV-2A (#RDB12341)

Expression vector of 2A peptide-linked proteins.

Alternative name pRC-2A
Clone info. Expression vector of 2A peptide-linked proteins.
Vector backbone pRc/RSV (Plasmid)
Selectable markers Amp^r
Depositor|Developer DNA Bank, | Nakade, Koji |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Acknowledgement to Developer in publication.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regrding pRc/RSV:
a) The modified pRc/RSV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 研究結果の発表時、謝辞に樹立者名を入れること。
利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
付加的使用条件：
pRc/RSVについて:
a) The modified pRc/RSV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

Catalog # Resource name Shipping form Fee
RDB12341 pRSV-2A DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRSV-2A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12341).

Reference section:

Kurihara, C., Nakade, K., Pan, J., Huang, J., Wasylyk, B., Obata, Y., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12341_A3Lp.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12341_A3Lpa.seq check
>RDB12341_A3Lp_RSV_Pro_F_D09_12.ab1
    1 TAGTTTGGAC ATTCACCACA TTGGGTGTGC ACCTCCAAGC TTGGTACCGA GCTCGGATCC
   61 ACTAGTGAAG GTCGTGGTTC TTTATTAACT TGTGGTGATG TTGAAGAAAA TCCTGGTCCT
  121 GCTAGCTGAG GTACCGCGGC CGCTCGAGCA TGCATCTAGA GCTCGCTGAT CAGCCTCGAC
  181 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT
  241 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT
  301 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG
  361 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG
  421 AACCAGCTGG GGCTCGAGGG GGGATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC
  481 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC
  541 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA
  601 TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT
661 TGATTAGGGT GATGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551. [link to RRC of NBRP]