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pgLTR_T5

gRNA expressing vector of HIV LTR

Catalog number RDB12749
Resource name pgLTR_T5
Alternative name T5 gRNA expressiong vector
Clone info. gRNA expressing vector. This gRNA was designed to target TAR region of HIV LTR.
Comment gRNA target sequence was cloned into the AflII site of the gRNA_cloning vector (Addgene #41824).
Vector backbone pCR-BluntII-TOPO (Plasmid)
Size of vector backbone 3.51kb
Gene/insert name Human U6 genomic DNA HIV-1 LTR genomic DNA
Depositor|Developer Koyanagi, Yoshio | Ebina, Hirotaka |
Other clones in our bank
External Database
Human U6
    HIV-1 LTR
            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Sci Rep. 3: 2510(2013)).
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci Rep. 3: 2510(2013))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB12749 pgLTR_T5 Under QC test. Please contact us. DNA solution Please contact us.

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Ebina, H., Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus. Sci. Rep. 3: 2510 (2013). PMID 23974631.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Ebina, H., Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus. Sci. Rep. 3: 2510 (2013). PMID 23974631.

            2022.05.17

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