Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFBD-Hs alpha1CGH/beta3CGF

Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I., FEBS Lett., 587, 3450-3455 (2013)) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (FEBS Lett., 587, 3450-3455 (2013))を引用し、謝辞の表明を必要とする。本件リソースは学術機関での学術研究への利用に限る。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請を行う場合は、受託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12753_A5Ftp1-2

Nucleotide sequence of a portion of this resource (if available).

Primer: pPolyhedrin_F () Sequence file: RDB12753_A5Fta.seq
>12753_12753_A5Ft_pPolyhedrin_F_B06_06.ab1     1 ATTTTTACTG TTTTCGTAAC AGTTTTGTAA TAAAAAAACC TATAAATATT CCGGATTATT    61 CATACCGTCC CACCATCGGG CGCGGATCCC GGTCCGAAGC GCGCGGAATT CAAAGGCCTA   121 CGTCGACGAG CTCACTAGTA ACTCCTAAAA AACCGCCACC ATGCGCGAGT GCATCTCCAT   181 CCACGTGGGC CAGGCTGGTG TCCAGATCGG CAACGCTTGC TGGGAGCTGT ACTGCCTGGA   241 GCACGGTATC CAGCCCGACG GCCAGATGCC CTCCGACAAG ACCATCGGTG GCGGCGACGA   301 CTCCTTCAAC ACCTTCTTCT CCGAAACCGG TGCTGGCAAG CACGTGCCCC GCGCTGTGTT   361 CGTGGATCTG GAACCCACCG TGATCGACGA AGTGCGTACC GGCACCTACC GTCAGCTGTT   421 CCACCCCGAG CAACTGATCA CCGGCAAGGA GGACGCTGCT AACAACTACG CTCGTGGTCA   481 CTACACCATC GGCAAGGAAA TCATCGACCT GGTCCTGGAC CGTATCCGCA AGCTGGCTGA   541 CCAGTGCACC GGCCTGCAGG GATTCCTCGT GTTCCACTCC TTCGGTGGTG GAACCGGTTC   601 TGGTTTCACC TCCCTGCTGA TGGAACGTCT GTCTGTGGAC TACGGCAAGA AGTCCAAGTT   661 GGAGTTCTCC ATCTACCCCG CTCCCCAGGT GTCCACCGCT GTGGTCGAGC CCTACAACTC   721 CATCCTGACC ACCCACACCA CCCTGGAACA CTCCGACTGC GCTTTCATGG TGGACAACGA   781 GGCTATCTAC GACATCTGCC GTCGTAACCT GGACATCGAG CGTCCCACCT ACACCAACCT   841 GAACCGTCTG ATCTCCCAGA TCGTGTCCTC CATCACCGCT TCCCTGCGTT TCGACGGTGC   901 TCTGAACGTG GACCTGACCG AGTTCCAGAC CAACCTGGTG CCCTACCCCC GTATCCACTT   961 CCCCCTGGCT ACCTACGCTC CCGTGATCTC CGCTGAGAAG GCTTACCACG AGCAGCTGTC  1021 CGTGGCTGAG ATCACCAAAC GCCTGCTTCG AGCCCGCTAA CCAGATGGTC AAGTGGCGAC  1081 CCCCCGTCAC GGCAGTACAT ACTTGCTGCC TGCCTGTAAC GTTACCGACG TTGATTGCTA 1141 AGACGTGAAC GGCTGCTATT CGGCCCTTAC CATCCAAGGA ACCTCCAAG //
Primer: ME-1290RV () Sequence file: RDB12753_A5Ftb.seq
>12753_12753_A5Ft_ME-1290Rv_C06_09.ab1     1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT    61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCCTCT AGTACTTCTC GACAAGCTTT   121 TAGTGGTGAT GGTGGTGATG ATGGTGACCA CCGGAACCAC CGTATTCCTC GCCTTCCTCT   181 TCGCCCTCGC CCTCGACGGA GTCGACGCCC ACTTCCTCGT AGTCCTTTTC CAGAGCAGCC   241 ATGTCCTCGC GAGCCTCGGA GAACTCGCCC TCTTCCATGC CTTCGCCCAC GTACCAGTGC   301 ACGAAAGCGC GCTTAGCGTA CATCAGGTCG AACTTGTGGT CCAGACGAGC CCAAGCCTCA   361 GCGATAGCGG TGGTGTTGGA CAGCATGCAC ACAGCACGCT GCACCTTAGC CAAGTCGCCA   421 CCGGGCACCA CAGTGGGGGG CTGGTAGTTG ATTCCGACCT TGAAACCGGT GGGGCACCAG   481 TCCACGAACT GGATGGAACG CTTGGTCTTG ATGGTAGCGA TAGCAGCGTT CACGTCCTTA   541 GGCACCACGT CGCCACGGTA CAGCAGGCAG CAAGCCATGT ACTTGCCGTG ACGGGGGTCG   601 CACTTGACCA TCTGGTTAGC GGGCTCGAAG CAGGCGTTGG TGATCTCAGC CACGGACAGC   661 TGCTCGTGGT AAGCCTTCTC AGCGGAGATC ACGGGAGCGT AGGTAGCCAG GGGGAAGTGG   721 ATACGGGGGT AGGGCACCAG GTTGGTCTGG AACTCGGTCA GGTCCACGTT CAGAGCACCG   781 TCGAAACGCA GGGAAGCGGT GATGGAGGAC ACGATCTGGG AGATCAGACG GTTCAGGTTG   841 GTGTAGGTGG GACGCTCGAT GTCCAGGTTA CGACGGCAGA TGTCGTAGAT AGCCTCGTTG 901 TCCACCATGA AAGCGCAGTC GGGAGTGTTC CAGGGTGGTG TGGGTGGTTC AGGATGGG //
Primer: pP10_F () Sequence file: RDB12753_A5Ftc.seq
>12753_12753_A5Ft_pP10_F_D06_12.ab1     1 TTATAGAATT ATTATCAAAT CATTTGTATA TTAATTAAAA TACTATACTG TAAATTACAT    61 TTTATTTACA ATCACTCGAC GAAGACTTGA TCACCCGGGA TCTCGAGAAC TCCTAAAAAA   121 CCGCCACCAT GCGCGAGATC GTGCACATCC AGGCTGGTCA GTGCGGTAAC CAGATCGGTG   181 CTAAGTTCTG GGAGGTCATC TCCGACGAGC ACGGTATCGA CCCTTCCGGT AACTACGTGG   241 GTGACTCCGA CCTGCAGCTG GAGCGCATTT CCGTGTACTA CAACGAAGCC TCCTCCCACA   301 AATACGTGCC TCGCGCTATC CTGGTGGACC TGGAGCCTGG TACTATGGAC TCTGTGCGCT   361 CCGGTGCTTT CGGTCACCTG TTCCGCCCTG ACAACTTCAT CTTCGGTCAG TCCGGTGCTG   421 GTAACAACTG GGCTAAGGGT CACTACACCG AGGGTGCTGA GCTCGTCGAC TCCGTGCTGG   481 ACGTGGTGCG CAAGGAGTGC GAGAACTGCG ACTGCCTGCA GGGTTTCCAG CTGACTCACT   541 CCCTGGGTGG TGGTACCGGA TCTGGTATGG GTACCCTGCT GATCTCCAAG GTGCGCGAGG   601 AGTACCCTGA CCGCATCATG AATACCTTCT CCGTCGTGCC TTCCCCTAAG GTGTCCGACA   661 CCGTGGTGGA GCCTTACAAC GCTACCCTGT CCATCCACCA ACTGGTGGAG AACACCGACG   721 AGACCTACTG CATCGACAAC GAGGCTCTGT ACGACATCTG CTTCCGCACC CTGAAGCTGG   781 CTACCCCTAC CTACGGTGAC CTGAACCACC TGGTCTCCGC TACCATGTCC GGTGTGACCA   841 CCTCCCTGCG CTTCCCTGGT CAGCTGAACG CTGACCTGCG CAAGCTGGCT GTGAACATGG   901 TGCCTTTCCC TCGCCTGCAC TTCTTCATGC CTGGTTTCGC TCCTCTGACC GCTCGCGGTT   961 CCCAGCAATA CCGCGCTCTG ACCGTGCCTG AGCTGACCCA GCAGATGTTC GACGCTAAGA  1021 ACATGATGGC TGCTTGCGAC CCTCGCCACG GGTCGCTACC TGACGTGGCT ACGTGTCCGC  1081 GGTCGCATGT CCATGATGAG TGGACGAGCA GATGCTGCTA TCAGTTCAGG ACTCTCCTAC 1141 ATCGTTGGAG TGCAATCCTA ACCAAACGGT GGAAAAAG //
Primer: HSV_TK_pA_R () Sequence file: RDB12753_A5Ftd.seq
>12753_12753_A5Ft_HSVTKpA_R_E06_15.ab1     1 ACGGTCTTCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCATCT CCCGGTACCG    61 CATGCTATGC ATCAGCTGCT AGCTTACTTG TCATCGTCAT CTTTGTAGTC ACCACCGGAA   121 CCACCCTTAG GACCCTGAGC CTCGGACTCT TCCTCATCGT CCTCGTACAT CTCACCCTCT   181 TCTTCAGCGG TAGCGTCCTG GTACTGCTGG TACTCGGACA CCAGGTCGTT CATGTTGGAC   241 TCAGCCTCGG TGAACTCCAT CTCGTCCATA CCCTCACCGG TGTACCAGTG CAGGAAAGCC   301 TTGCGGCGGA ACATAGCGGT GAACTGCTCG GAGATGCGCT TGAACAGCTC CTGGATAGCG   361 GTGGAGTTAC CGATGAAGGT GGAGGACATC TTCAGACCGC GAGGAGGGAT GTCGCACACA   421 GCCACCTTCA CGTTGTTAGG GATCCACTCC ACGAAGTAGG AGGAGTTCTT GGACTGGATA   481 GCCAGCATCT GCTCGTCCAC CTCCTTCATG GACATGCGAC CGCGGAACAC GGTAGCCACG   541 GTCAGGTAGC GACCGTGGCG AGGGTCGCAA GCAGCCATCA TGTTCTTAGC GTCGAACATC   601 TGCTGGGTCA GCTCAGGCAC GGTCAGAGCG CGGTATTGCT GGGAACCGCG AGCGGTCAGA   661 GGAGCGAAAC CAGGCATGAA GAAGTGCAGG CGAGGGAAAG GCACCATGTT CACAGCCAGC   721 TTGCGCAGGT CAGCGTTCAG CTGACCAGGG AAGCGCAGGG AGGTGGTCAC ACCGGACATG   781 GTAGCGGAGA CCAGGTGGTT CAGGTCACCG TAGGTAGGGG TAGCCAGCTT CAGGGTGCGG   841 AAGCAGATGT CGTACAGAGC CTCGTTGTCG ATGCAGTAGG TCTCGTCGGT GTTCTCCACC   901 AGTTGGTGGA TGGACAGGGT AGCGTTGTAA AGCTCCACCA CGGTGTCGGA CACCTTATGG   961 GAAGGGCACG ACGGAGAAGG TATTCATGAT GCGGTCAGGG TACTCCTCGC GCACCTTGGA  1021 GATCAGCAGG TACCCATACC AGATCCGGTA CCACCAACCC AGGGAGTGAG TCAGCTGGAA  1081 AACCCCTGCA GCAGTCGCAG TCTCGCCACT CCTTGCGCAC ACGTTCAGCA CGAGTTCGAA 1141 CGAGCTCAGC ACCCCTCTCG GGTGTTAGTG AACCCCTTAT AGTCCCCAAG T //
Primer: pBACe3.6_F () Sequence file: RDB12753_A5Fte.seq
>12753_12753_A5Ft_pBACe3.6_F_F06_18.ab1     1 TGTAGCCTGC GTAGCGGGTG TGGGCGGACA ATAAAGTCTT AAACTGAACA AAATAGATCT    61 AAACTATGAC AATAAAGTCT TAAACTAGAC AGAATAGTTG TAAACTGAAA TCAGTCCAGT   121 TATGCTGTGA AAAAGCATAC TGGACTTTTG TTATGGCTAA AGCAAACTCT TCATTTTCTG   181 AAGTGCAAAT TGCCCGTCGT ATTAAAGAGG GGCGTGGCCA AGGGCATGGT AAAGACTATA   241 TTCGCGGCGT TGTGACAATT TACCGAACAA CTCCGCGGCC GGGAAGCCGA TCTCGGCTTG   301 AACGAATTGT TAGGTGGCGG TACTTGGGTC GATATCAAAG TGCATCACTT CTTCCCGTAT   361 GCCCAACTTT GTATAGAGAG CCACTGCGGG ATCGTCACCG TAATCTGCTT GCACGTAGAT   421 CACATAAGCA CCAAGCGCGT TGGCCTCATG CTTGAGGAGA TTGATGAGCG CGGTGGCAAT   481 GCCCTGCCTC CGGTGCTCGC CGGAGACTGC GAGATCATAG ATATAGATCT CACTACGCGG   541 CTGCTCAAAC TTGGGCAGAA CGTAAGCCGC GAGAGCGCCA ACAACCGCTT CTTGGTCGAA   601 GGCAGCAAGC GCGATGAATG TCTTACTACG GAGCAAGTTC CCGAGGTAAT CGGAGTCCGG   661 CTGATGTTGG GAGTAGGTGG CTACGTCTCC GAACTCACGA CCGAAAAGAT CAAGAGCAGC   721 CCGCATGGAT TTGACTTGGT CAGGGCCGAG CCTACATGTG CGAATGATGC CCATACTTGA   781 GCCACCTAAC TTTGTTTTAG GGCGACTGCC CTGCTGCGTA ACATCGTTGC TGCTGCGTAA   841 CATCGTTGCT GCTCCATAAC ATCAAACATC GACCCACGGC GTAACGCGCT TGCTGCTTGG   901 ATGCCCGAGG CATAGACTGT ACAAAAAAAC AGTCATAACA AGCCATGAAA ACCGCCACTG   961 CGCCGTTACC ACCGCTGCGT TCGGTCAAGG TTCTGGACCA GTTGCGTGAG CGCATACGCT  1021 ACTTGCATTA CAGTTTACGA CCGAACAGCT TATGTCACTG GGATCGTGCT CATCGTTTCA  1081 CGGTGTGCGT CACCGCCAGT GGCAGCAGCG AGTCGAGGCA TTCCTGTCGT GGTCTGCGAC 1141 CGAGCGGCCA AGGATTCGGT CTTCCAACGG //
Primer: pGCAP_R () Sequence file: RDB12753_A5Ftf.seq
>12753_12753_A5Ft_pGCAP_R_G06_21.ab1     1 AGAATCTTAT CATGTCTGGA TCTGATCACT GCTTGAGCCT AGGAGATCCG AACCAGATAA    61 GTGAAATCTA GTTCCAAACT ATTTTGTCAT TTTTAATTTT CGTATTAGCT TACGACGCTA   121 CACCCAGTTC CCATCTATTT TGTCACTCTT CCCTAAATAA TCCTTAAAAA CTCCATTTCC   181 ACCCCTCCCA GTTCCCAACT ATTTTGTCCG CCCACAGCGG GGCATTTTTC TTCCTGTTAT   241 GTTTTTAATC AAACATCCTG CCAACTCCAT GTGACAAACC GTCATCTTCG GCTACTTTTT   301 CTCTGTCACA GAATGAAAAT TTTTCTGTCA TCTCTTCGTT ATTAATGTTT GTAATTGACT   361 GAATATCAAC GCTTATTTGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT   421 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG   481 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC   541 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC   601 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT   661 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TAATAGTGGA CTCTTGTTCC AAACTGGAAC   721 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC   781 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT   841 AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA   901 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT   961 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTTATTCC  1021 CTTTTTTGCG GCATTTTGCC TTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAGTAAA  1081 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTACATCGA CTGGATCTCA CAGCGTAGGA 1141 TTCATGAAGT TTTCGCTCTC GAGAACGGTT TTTCCAATGA TGGAGAGCAC AC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl