Please review the QC test results indicated by icon below as well as clone information before placing your order.

Histac-K12 pcDNA3.1

For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Ito, T. et al., ChemBio. 18, 495-507, (2011)). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ito, T. et al., ChemBio. 18, 495-507, (2011))。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12841_A4Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward () Sequence file: RDB12841_A4Lpa.seq
>12841_12841_A4Lp_CMV-Forward_D12_12.ab1     1 CGTTACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC   121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CATGGTGAGC   181 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA   241 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG   301 ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC   361 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC   421 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC   481 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC   541 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG   601 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG   661 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC   721 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC   781 TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG   841 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGGA ATTCACCCCT   901 GCCAACCCAC CACCCCCCGG AGGTGTCCAA TCCCAAAAAG CCAGGACGAG TTACCAACCA   961 GCTGCAATAC CTACACAAGG TAGTGATGAA AGGCTCTGTG GAAACATCAG TTCGCATGGC  1021 CCATTCCCGG CAGCCCTGTG GATGCTGTCA AACTGGGGTC TTTCCCGGGA TATCACAAAA  1081 ATTATAAAAC AGGCCTATGA CATTGGTTAC TAATTAAGAG GAGACTTGAA AAACAACTAC 1141 TAACTGCGC //
Primer: BGH_rev () Sequence file: RDB12841_A4Lpb.seq
>12841_12841_A4Lp_BGHrev_E12_15.ab1     1 GCCGACGGGG GCTCTAGACT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC    61 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT   121 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT   181 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG   241 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG   301 GCTGATGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT   361 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT   421 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT   481 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC   541 GCCCCAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA   601 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT   661 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA   721 CAGCTCCTCG CCCTTGCTCA CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC   781 GCCGCCGAAG CCGTACAGAG TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC   841 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC   901 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC   961 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGTA ATGCCCTGAT GTTGTCGCGA  1021 GAACCTTTGC GGTGTCGCTT GCGCCACCTT TCCCCAGACC CTTTGCCGCC TTTTCTCGTC  1081 CTGACATGAT CAGATTCTTC ACACTGGATC ACACCTCAGG ATCTCGTCCA CCAGGAATCC 1141 AACCTTCT //
Primer: SV40pro_F () Sequence file: RDB12841_A4Lpc.seq
>12841_12841_A9Cr_SV40pro_F_A03_03_ABI24.ab1     1 ACTCGTCCCT GGAACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA    61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT   121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA   661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT   781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG   841 ACCGCTTCCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT   901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA   961 CGCCCAACCT GCCATCACGA GGATTTCGAT TCCACCGCCG CCTTCTATGA AAGTTGGGCT  1021 TCGGAATCGT TTCTGGACGC TGCCTGGATG ATTCTTCAGC GCGGGATCTC ATGCTGAGTC 1081 TGCCAACCCC AACTGTTAAT TGGCAAGCTT TATAAATTGG CTA //
Primer: attB2-R1 () Sequence file: RDB12841_A4Lpd.seq
>12841_12841_A9Cr_attB2-R1_B03_06_ABI24.ab1     1 GCCCAAACGG AAAGAAGTGG CGGGCCCTGG GCGCTTGAGC GCGTACACCA CGTCCATAGC    61 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC   121 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC   181 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGTA ATGCCCTGGA TGTTGTCGCG   241 GAGAACCTTG CGGTGTCGCT TGGCGCCACC TTTCCCCAGA CCCTTGCCGC CTTTTCCTCG   301 TCCTGACATG GATCCAGATC CTCCACCACC TGAACCACCA CCTCCAGAAC CTCCTCCACC   361 AGATCCACCT CCTCCGTCGA CGGCAGTAGA GACTGGTAAA GGCCCTGGTT CTAGTGGTTC   421 ATCTGTCTTC TTGGCATAAC GGAACTCAAA TACATCCTGT AGCTTTCGTG CCATTGCCAC   481 AACATCGTGA TCTGGGGGAT TGTACTTATA GCAGTTGGAG AACATAAGCC GTACATCAGC   541 AGCAAACTCC TGTGCATCCC GGTAATCACG GTTCTCCATC TTCCGCTTGA CAGTGCTGAG   601 GTCCATGGGG TGCTTAATGA TGTCATGGTA GTCATGCAGG CCAAGTGCAG AAGCATCCAC   661 TGGTTTATAG AAAGGCCAAG CATAGGCAGC ATGCTTCTTA GAAGAGTAAC TCCTTCAAAT   721 GCCATTGGCA AATGTTTTAA CTGTTCTGAA AGCTTTCCTT TCTTAGAGGC TCTGGTGTTG 781 TTGCTGAGAG TCAGGCAAGT CTTTTCGTGG GGGCTTGATG GGGCGAACCA ACTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl