Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSIV-TRE-RfA-CMV-KT

Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12876_A4Klp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: KO-M_R (Pr0821) Region: hKO1 5', CMV pro2 Sequence file: RDB12876_A4Kla.seq
>12876_12876_B0BiS_KO-M_R1_A06_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTGGNNGCT CCTCTCCNNG CTCNGGCCCT CGGGGAAGGC    61 CTGCTTGAAG TAGTCGGGGN NCTCCTCGGG GTACTTGGTG AAGGGCCTGT GGCCGTAGCA   121 GAAGGTGTGG CTCACCAGGT CGAAGCTGAA GGGCATGGGG CCGCCCTTGG CCATTGTCAC   181 CCTCAGGGTC ATCTCCTGGT GGCCCTCGTA GGGCTTGCCG GTGCCCTCGC CCTCCACGGT   241 GAACTCGTGG CCGTTCACGC TGCCGTCCAT GAAGTACTTC ATCTTCATCT CGGGCTTGAT   301 CACGCTCACC ATGGTGGACC GGTGAATTCG GGGCCGCGGA GGCTGGATCG GTCCCGGTGT   361 CTTCTATGGA GGTCAAAACA GCGTGGATGG CGTCTCCAGG CGATCTGACG GTTCACTAAA   421 CGAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG   481 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCNAAA CAAACTCCCA   541 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA   601 TTGATGTACT GCCNAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC   661 TGCCNAGTAG GAAAGTCCCA TAANGTCATG TACTGGGCAT AATGNCAGGC GGGCCATTTA   721 CCGTCATTGA CGTCAATANG GGGCGTACTN NGCATATGAT ACACTTGATG TACTGNNNAN   781 NGGGCAGTTT ACCGNAAATA CTCCACCNNT NNNCNTCNNN GGNAAGNNCN NATNNNNNTT   841 ACTATGGGNN NNTACNNCNN TNNNTNGANN NNNNNNGGCN GGGGNNNNNN GGCNNNCANC 901 CNNNNNGGNC NNNNNNNNNN //
Primer: hKO_F (Pr0157) Region: T2A, rtTA-Ad Sequence file: RDB12876_A4Klb.seq
>12876_12876_A4Kl_2_hKO_F_A10_01.ab1     1 NNNNNNNNNN NNNGCNNGNN NNGANNNCGA NGGCAACATC ACNNNNNNGG TGGAGGANGC    61 CGTGGCCCAC TGNNNNNNCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC   121 CGGCCCTATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGCGCTCTGG AATTACTCAA   181 TGGAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA   241 GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA   301 GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT   361 TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA   421 AGTGCATCTC GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC   481 GTTCCTGTGT CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG   541 CCACTTTACA CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG   601 AGAGACACCT ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA   661 CCGGCAGGGA GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA   721 GAAACAGCTA AAGTGCGAAA GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTANA   781 CATGCTCCCA GCCGATGCCC TTGNCGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT   841 TGACGANTTT GACCTTGACA TGCTCCCCGG GNAACTAAGT AANGNTCCTC TAGAGTTAAC   901 ATCNAGGGNT CNAGCTTATC GATAATCAAC NNCNNGATTA CNAAANTNGN NAAANANTGN 961 CNGGNANNNN NNNNTATGNT GNNNNNTTTN NNNNNNNNN //
Primer: Cm_N_rev (Pr0014) Region: attR1, tight TRE pro, cPPT_CTS Sequence file: RDB12876_A4Klc.seq
>12876_12876_A4Kl_2_Cm_N_rev_B10_04.ab1     1 NNNNNNNNNN NNTNTTNNNG NNGCNNTTGG GNNNTATCAA CNNNNNNATA TCCNGTGATT    61 TTTTTCTCCN NNNNNNNNTC CTTAGCTCCT GAAAATCTCG ACGGATCCTA ACTCAAAATC   121 CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC   181 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT   241 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTT TTGTACAAAC   301 TTGTGATGCT AGCGGCGATC TGACGGTTCA CTAAACGAGC TCTGCTTATA TAGGCCTCCC   361 ACCGTACACG CCTACCTCGA CATACGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA   421 CGTTCTCTAT CACTGATAGG GATAAACTCG ACATACGTTC TCTATCACTG ATAGGGAGTA   481 AACTCGACAT ACGTTCTCTA TCACTGATAG GGAGTAAACT CGACATACGT TCTCTATCAC   541 TGATAGGGAG TAAACTCGAC ATCGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG   601 TTCTCTATCA CTGATAGGGA GTAAACTCGA TTGAAGACGA AAGGGCCGAA TTCAAAGTGA   661 ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA   721 TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC   781 CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGANTACT GCCATTTGTA GATCCGTTCA   841 CTAATCNAAT GGATCTNNCT CTGNCTCTCT CTNCNCCTTC NNCNNNTATT NCNNCNGNNC   901 TGTCNGNNCC CNNNNGGNNN NGNANGNNGN NNTGAANCNA NNNNGGNNNN NNTNCNNNNN 961 NNANTNNNNN NNNNN //
Primer: CS-CMVen_upR (Pr0822) Region: attR2, ccdB Sequence file: RDB12876_A4Kld.seq
>12876_12876_B0BiS_CS-CMVen_upR_B06_02_ABI08.ab1     1 NNNNNNNNNN NNNANNGANN NGGNNNNNNN NTATGCAATG GGCCNAGCTC CTCGAGACCG    61 GTAGCATCAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA   121 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA   181 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT   241 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC   301 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG   361 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA   421 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT   481 ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT   541 TCACCAGCCC CTGTTCTCGT CAGCAAAAGA GCCGTTCATT TCAATAAACC GGGCGACCTC   601 AGCCATCCCT TCCTGATTTT CCGCTTTCCA GCGTTCGGCA CGCAGACGAC GGGCTTCATT   661 CTGCATGGTT GTGCTTACNN GACCGGANAT ATTGACATCA TATATGCCTT GANCAACTGA   721 TAGCTGTCGC TGTCAACTGT CACTGTAATA CGCTGCTTCN TANCATACCT CTTTTTGACA   781 TACTTCNGGT ATACNTATCA GTATATATTC NTATANCGCA AAAATCANCG CGCAAATACN 841 CATACNGNNN TCTGGNTTTN ANNNNNNCNG NNNNNNCGCN NTNNNNNNNN NN //
Primer: tTA-Ad R (Pr0542) Region: T2A, hKO1 3' Sequence file: RDB12876_A4Kle.seq
>12876_12876_B0BiS_tTA-Ad_R_C06_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT NNNNTCTTGT CNNGTCTAGA CATAGGGCCG    61 GGNTTCTCCT CCACGTCACC GCATGTTAGA AGACTTCCTC TGCCCTCGCA GTGGGCCACG   121 GCGTCCTCCA CCAGCTCGGT GATGTTGCCC TCGGTCTTCC TCACCAGCCT GTGGCCGATG   181 AAGTGGCTCT GGGGCATCTT CAGGATCTTC TTGGCGGCCT TGTAGGTGGT CTTGAACTGG   241 CACTTGTGGT TGCCGCCGCC GGCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG   301 CCGTCGCAGG TGGTGATCTT CTCGGTGCTG GGCTCCCAGT CGCTGCTCTG GTTCTGCATC   361 ACGGGGCCGT CGGCGGGGAA GTTCACGCCC ACGAACTTGC TCTTGTGCTC GAAGCAGTTG   421 CCCCTCAGGC TGATGTGGGC GCTCACGGCG GCGAAGCCGC CGTCCTCGAA CTGGAGGCTC   481 CTCTCCCAGC TCAGGCCCTC GGGGAAGGCC TGCTTGAAGT AGTCGGGGAT CTCCTCGGGG   541 TACTTGGTGA AGGGCCTGTG GCCGTAGCAG AANGTGTGGC TCACCAGGTC GAAGCTGAAG   601 GGCATGGGGC CGCCCTTGGC CATTGTCACC CTCAGGGTCA TCTCCTGNTG GCCCTCGTAN   661 GGCTTGCCGG TGCCCTCGCC CTCCACGGTG AACTCGTGGC CGTTCACGCT GCCGTCCATG   721 AAGTACTTCA TCTTCATCTC GGGCTTGATC ACGCTCACNA TGGTGGNNCG GTGAATTCNG   781 GGCCGCGGNN GCTGGATCGG TCCCGGTGTC TTCTATGGAN GTCAAAACAG CGTGGATGGC   841 GTCTCCNNGC GATCTGANGG TTCACTNAAN NGANCTNTGN TNATATAGAN CNNCNNCCGN   901 ACNCGCNNNN CNNCNNNTTG NNNCNATGGG NNNGNANNNG NTNNNANNTT TNGGAANNTC 961 CNNNNNNNTT NNNNNNNNNA ANNNNNNNNC NNNNTNNNNN NNNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi, 5' LTR Sequence file: RDB12876_A4Klf.seq
>12876_12876_A4Kl_2_HIVgp_R1_F10_16.ab1     1 NNNNNNNNNT NNNNNNNNNC TTTTTTCTCT CTGCACCNCT CTNCNCTTTG CCTTGGTGGG    61 TGCTACTNNN NNNNNNNNAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT   121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT   181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA   241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG   301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT   361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT   421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA   481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG   541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG   601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT   661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGANGG ATCTCTAGTT   721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG   781 ANGCTTAAGC AGTGGGTTCN CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA   841 CCAGANAGAC NNAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCNAC CNTACACGCC   901 NANCGCCCAT TTGCGTCAAT GGGGCGGANT NNNNACNANA TTTNNNAAAG TCCCNNNNAN   961 TTNNNNNNNN AAACAAANTC CNNTTNNNNN NNNNGGGGGN NNNNNNTNNN NNANNNNNNN 1021 NNN //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), 3'LTR, loxP, PRE Sequence file: RDB12876_A4Klg.seq
>12876_12876_B0BiS_BGH_rev2_D06_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGAATAA CTTCGTATAA   301 TGTATGCTAT ACGAAGTTAT GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT   361 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT   421 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG   481 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG   541 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGAGGGCGA   601 AGGCGAAGAC GCGGAAGAGG CCGCAGAGCC GGCAGCAGGC CGCGGGAAGG AAGGTCCGCT   661 GGATTGAGGG CCGAAGGGAC GTAGCAGAAG GACGTCCCGC GCAGAATCCA GGTGGCAACA   721 CAGGCGAGCA GCCATGGAAA GGACGTCAGC TTCCCCGACA ACACCACGGA ATTGTCAGTG   781 CCCAACAGCC GANCCCCTGT CCAGCAGCGG GCAAGGCAGG CGGCGANGAN TTCCGCCGNG   841 GCAATANGGA NGGGGGAAAG CGAAAGTCCC GGAAAGGANC NNANAGGNGG NGGCNNNGCC   901 CCNANCNNNG GGGGGTTGCG NCAGCAAACN CNNNGCNNAN NNNNNCNNNN NTNNNCNNGA 961 NNNNGGNNNN NNNNNN //
Primer: SV40pro_F (Pr0110) Region: Zeo, SV40 p(A) Sequence file: RDB12876_A4Klh.seq
>12876_12876_A4Kl_2_SV40pro_F_E10_13.ab1     1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GCTTTTTTGG AGGCCNNNNN NTTTGCAAAA    61 AGCTCCCGGG AGCNNGNNNN NNNNTTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT   601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA   661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GNTTACNAAT AAAGCAATAG   721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCNCTGCAT TCTAGTTGTG GTTTGTCCNA   781 ACTCNTCNAT GTATCTTATC ATGTCTGTAT ANNGTCNNNC TCTANCTAGA NCTTGGCGTA   841 ANCATGGNCA TAGCTGNTTC CTGNGTGAAA TTGNTATCCN NTCNCNATTC CNNNCNANAT   901 ACNANCNNGN NNNATAAAGN GNNANNNNNG GGNNGNNNNN NGNNTGNNCT NNCNNNNNNN 961 NANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.04.04

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