Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-hOct3/4-IRES2-Venus

Lentivirus vector plasmid of human OCT3/4 for generating iPS cells.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12908_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 Sequence file: RDB12908_A4Jfa.seq
>12908_12908_A4Jf_pAxEF_F2_1_D02_11.ab1     1 ATCTTCATTT AGTGTCGTGA CACGCTACCG GTCTCGAGAA TTCTGCAGCG GCCATCACAA    61 GTTTGTACAA AAAAGCAGGC TCCGCGGCCG CCCCCTTCAC CATGGCGGGA CACCTGGCTT   121 CGGATTTCGC CTTCTCGCCC CCTCCAGGTG GTGGAGGTGA TGGGCCAGGG GGGCCGGAGC   181 CGGGCTGGGT TGATCCTCGG ACCTGGCTAA GCTTCCAAGG CCCTCCTGGA GGGCCAGGAA   241 TCGGGCCGGG GGTTGGGCCA GGCTCTGAGG TGTGGGGGAT TCCCCCATGC CCCCCGCCGT   301 ATGAGTTCTG TGGGGGGATG GCGTACTGTG GGCCCCAGGT TGGAGTGGGG CTAGTGCCCC   361 AAGGCGGCTT GGAGACCTCT CAGCCTGAGG GCGAAGCAGG AGTCGGGGTG GAGAGCAACT   421 CCGATGGGGC CTCCCCGGAG CCCTGCACCG TCACCCCTGG TGCCGTGAAG CTGGAGAAGG   481 AGAAGCTGGA GCAAAACCCG GAGGAGTCCC AGGACATCAA AGCTCTGCAG AAAGAACTCG   541 AGCAATTTGC CAAGCTCCTG AAGCAGAAGA GGATCACCCT GGGATATACA CAGGCCGATG   601 TGGGGCTCAC CCTGGGGGTT CTATTTGGGA AGGTATTCAG CCAAACGACC ATCTGCCGCT   661 TTGAGGCTCT GCAGCTTAGC TTCAAGAACA TGTGTAAGCT GCGGCCCTTG CTGCAGAAGT   721 GGGTGGAGGA AGCTGACAAC AATGAAAATC TTCAGGAGAT ATGCAAAGCA GAAACCCTCG   781 TGCAGGCCCG AAAGAGAAAG CGAACCAGTA TCGAGAACCG AGTGAGAGGC AACCTGGAGA   841 ATTTGTTCCT GCAGTGCCCG AAACCCACAC TGCAGCAGAT CAGCCACATC GCCCAGCAGC   901 TTGGGCTCGA GAAGGATGTG GTCCGAGTGT GGGTTCTGTA ACCGGCGCCA GAAGGCAAGC   961 GATCAAGCAG CGACTATGCA CAACGAGAGG ATTTTGAGGC TGCTGGGTCT CCTTTTCTCA  1021 GGGGGACAGT GTCCTTTTCT CTGGGCCCCA GGACCCATTT TGCTACCCCA GCCTATGGCA 1081 GTCCGTTCAC TGCACTGGCA CTGTACTCCC TTCGGTTCCC A //
Primer: IRES-R Sequence file: RDB12908_A4Jfb.seq
>12908_12908_A4Jf_IRES_R_1_H02_23.ab1     1 GCAAAACCGG GCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG    61 CCATCACCAC TTTGTACAAG AAAGCTGGGT CGGCGCGCCC ACCCTTACCT CAGTTTGAAT   121 GCATGGGAGA GCCCAGAGTG GTGACGGAGA CAGGGGGAAA GGCTTCCCCC TCAGGGAAAG   181 GGACCGAGGA GTACAGTGCA GTGAAGTGAG GGCTCCCATA GCCTGGGGTA CCAAAATGGG   241 GCCCTGGGGC CAGAGGAAAG GACACTGGTC CCCCTGAGAA AGGAGACCCA GCAGCCTCAA   301 AATCCTCTCG TTGTGCATAG TCGCTGCTTG ATCGCTTGCC CTTCTGGCGC CGGTTACAGA   361 ACCACACTCG GACCACATCC TTCTCGAGCC CAAGCTGCTG GGCGATGTGG CTGATCTGCT   421 GCAGTGTGGG TTTCGGGCAC TGCAGGAACA AATTCTCCAG GTTGCCTCTC ACTCGGTTCT   481 CGATACTGGT TCGCTTTCTC TTTCGGGCCT GCACGAGGGT TTCTGCTTTG CATATCTCCT   541 GAAGATTTTC ATTGTTGTCA GCTTCCTCCA CCCACTTCTG CAGCAAGGGC CGCAGCTTAC   601 ACATGTTCTT GAAGCTAAGC TGCAGAGCCT CAAAGCGGCA GATGGTCGTT TGGCTGAATA   661 CCTTCCCAAA TAGAACCCCC AGGGTGAGCC CCACATCGGC CTGTGTATAT CCCAGGGTGA   721 TCCTCTTCTG CTTCAGGAGC TTGGCAAATT GCTCGAGTTC TTTCTGCAGA GCTTTGATGT   781 CCTGGGACTC CTCCGGGTTT TGCTCCAGCT TCTCCTTCTC CAGCTTCACG GCACCAGGGG   841 TGACGGTGCA GGGCTCCGGG GAGGCCCCAT CGGAGTTGCT CTCCACCCCG ACTCCTGCTT   901 CGCCCTCAGG CTGAGAGGTC TCCAAGCCGC CTTGGGGCAC TAGCCCCACT CCAACCTGGG   961 GCCCACAGTA CGCCATCCCC CCACAGAACT CATACGGCGG GGGGGCATGG GGGGAATCCC  1021 CACACTCAGA GCCTGACCAA CCCCCGGGCC GATTCCTGGT CCTCCAGGAG GGCTTGAGCT  1081 AAGCAGGATC CGAGGATCAC CAAGCCGCTT CCGACTCCTT GACCATTCAC TTCAACCATC 1141 CTTGG //
Primer: IRES-F Sequence file: RDB12908_A4Jfc.seq
>12908_12908_A4Jf-IRES_F_1_E02_14.ab1     1 GCCTTCCTTT TCATGTGTTT AGTTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG    61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG   121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG   181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC   241 TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC   301 TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT   361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG   421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG   481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA   541 ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA   601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC   661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC   721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG   781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGAA TTCAAGGCCT   841 CTCGAGCCTC TAGAGTTAAA CATCGAGGGA TCAAGCTTAT CGATAATCAA CCTCTGGATT   901 ACAAAATTTG TGAAAGATTG ACTGGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT   961 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC  1021 TCCTCCTTGT ATAATCCTGG GTTGCTGTCT CTTATGAGAG GTTGTGGCCC GTTGTCAGCC  1081 ACGTGGCCGT ATGTGCACTG TGTTTGGCTG ACGCACCCCC CCACTGTTGG GGCCATTGGC 1141 CAACCCCA //
Primer: CMV-Forward Sequence file: RDB12908_A4Jfd.seq
>12908_12908_A4Jf_CMV_Forward_1_C02_08.ab1     1 CGTTAAACTC GGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT   121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA   181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA   241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA   301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG   361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT   421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG   481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG   541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA   601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT   661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT   721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG   781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT   841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG   901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA   961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTTGTC  1021 TGGGTATAGT GCAGCAGCCA GAACAATTTG CTGAAGGGGC TATTGAGCGC AACCAGCCAT 1081 CTGGTTGGCA CTCACAGTCT GGGGGCATCC AAGCAGCTCC AAGGGCCAAA GGA //
Primer: BGH_rev Sequence file: RDB12908_A4Jfe.seq
>12908_12908_A4Jf_BGH_rev_1_F02_17.ab1     1 GCTAGACGGG ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT    61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT   121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA   181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC   241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA   301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA   361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG   421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT   481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC   541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA   601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG   661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG   721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA   781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC   841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG   901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC   961 AGCGGGCAAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA TAGGGAGGGG GAAAGCGAAA  1021 GTCCCGGGAA GGAGCTGACA GGTGGTGGCA ATGCCCCAAC CAGTGGGGGG TTGCGTCAGC  1081 AAACACAGTG CACACACGTC ACGATTGCTG ACACGGACAC ACTTCTTCAT AAGAGACAGC 1141 CAACCAAGGG ATTTTATTAC A //
Primer: SV40pro_F Sequence file: RDB12908_A4Jff.seq
>12908_12908_A4JF_SV40pro_F_1_G02_20.ab1     1 GCTTGGTCCT GGACATCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA   121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC   181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC   241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC   301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC   361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG   421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG   481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG   541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC   601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG   661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT   721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC   781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG   841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC   901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC   961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA  1021 TTAATGAAAT CGGCCCAACG GCGCGGGGGA GAGGCGGTTT GCGTATTGGG CGGCTCTTCG  1081 CCTTCTCGCT CACTGAACTC GCTGCGCTCG GATCGATCGG CTGCGGCGAG TCGGATCAGC 1141 TCCATCATAG GGCTCGGTTT A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl