Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-hc-Myc-IRES2-Venus

Lentivirus vector plasmid of human MYC for generating iPS cells.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12911_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 Sequence file: RDB12911_A4Jfa.seq
>12911_12911_A4Jf_pAxEF_F2_1_F04_16.ab1     1 GATTTCTCTC CTTTTAGGGT GTCGTGAACA CGCTACCGGT CTCGAGAATT CTGCAGCGGC    61 CATCACAAGT TTGTACAAAA AAGCAGGCTC CGCGGCCGCC CCCTTCACCA TGCCCCTCAA   121 CGTTAGCTTC ACCAACAGGA ACTATGACCT CGACTACGAC TCGGTGCAGC CGTATTTCTA   181 CTGCGACGAG GAGGAGAACT TCTACCAGCA GCAGCAGCAG AGCGAGCTGC AGCCCCCGGC   241 GCCCAGCGAG GATATCTGGA AGAAATTCGA GCTGCTGCCC ACCCCGCCCC TGTCCCCTAG   301 CCGCCGCTCC GGGCTCTGCT CGCCCTCCTA CGTTGCGGTC ACACCCTTCT CCCTTCGGGG   361 AGACAACGAC GGCGGTGGCG GGAGCTTCTC CACGGCCGAC CAGCTGGAGA TGGTGACCGA   421 GCTGCTGGGA GGAGACATGG TGAACCAGAG TTTCATCTGC GACCCGGACG ACGAGACCTT   481 CATCAAAAAC ATCATCATCC AGGACTGTAT GTGGAGCGGC TTCTCGGCCG CCGCCAAGCT   541 CGTCTCAGAG AAGCTGGCCT CCTACCAGGC TGCGCGCAAA GACAGCGGCA GCCCGAACCC   601 CGCCCGCGGC CACAGCGTCT GCTCCACCTC CAGCTTGTAC CTGCAGGATC TGAGCGCCGC   661 CGCCTCAGAG TGCATCGACC CCTCGGTGGT CTTCCCCTAC CCTCTCAACG ACAGCAGCTC   721 GCCCAAGTCC TGCGCCTCGC AAGACTCCAG CGCCTTCTCT CCGTCCTCGG ATTCTCTGCT   781 CTCCTCGACG GAGTCCTCCC CGCAGGGCAG CCCCGAGCCC CTGGTGCTCC ATGAGGAGAC   841 ACCGCCCACC ACCAGCAGCG ACTCTGAGGA GGAACAAGAA GATGAGGAAG AAATCGATGT   901 TGTTTCTGTG GAAAAGAGGC AAGCTCCTGA CAAAAGGTCA GAGTCTGGAT CACCCTTCTG   961 CTGGAGGCCA CAGCAAACCT CCCTCACAGC CCACTGGTCC TCAAGAGGTG CCACGGTCTC  1021 CACACATCAG CACAACTACG CAGCGGCCTC CCTCCACTCG GAAAGGACTA TCCTTGCTGC  1081 CAAGAAGGGT CCAAGTTGGG AACAGTGTTC AGAAGTCCTT GAAAACGATC AGCTACCAAC  1141 CGAAATGCAC CAGCCCCTCA GGGTCCCTTC GGACACTCGA GAGGAAGAAA TTGTTCTCAG 1201 AGGGGGCGCG TATATCACA //
Primer: IRES-R2 Sequence file: RDB12911_A4Jfb.seq
>12911_12911_A4Jf_1_IRES-R2_A09_03.ab1     1 GAGCCCATCA GGCGGGCTTC GGCAGTAACG TTAGGGGGGG GGGAGGGAGA GGGGCGGATC    61 CGCGGCCATC ACCACTTTGT ACAAGAAAGC TGGGTCGGCG CGCCCACCCT TTACGCACAA   121 GAGTTCCGTA GCTGTTCAAG TTTGTGTTTC AACTGTTCTC GTCGTTTCCG CAACAAGTCC   181 TCTTCAGAAA TGAGCTTTTG CTCCTCTGCT TGGACGGACA GGATGTATGC TGTGGCTTTT   241 TTAAGGATAA CTACCTTGGG GGCCTTTTCA TTGTTTTCCA ACTCCGGGAT CTGGTCACGC   301 AGGGCAAAAA AGCTCCGTTT TAGCTCGTTC CTCCTCTGGC GCTCCAAGAC GTTGTGTGTT   361 CGCCTCTTGA CATTCTCCTC GGTGTCCGAG GACCTGGGGC TGGTGCATTT TCGGTTGTTG   421 CTGATCTGTC TCAGGACTCT GACACTGTCC AACTTGACCC TCTTGGCAGC AGGATAGTCC   481 TTCCGAGTGG AGGGAGGCGC TGCGTAGTTG TGCTGATGTG TGGAGACGTG GCACCTCTTG   541 AGGACCAGTG GGCTGTGAGG AGGTTTGCTG TGGCCTCCAG CAGAAGGTGA TCCAGACTCT   601 GACCTTTTGC CAGGAGCCTG CCTCTTTTCC ACAGAAACAA CATCGATTTC TTCCTCATCT   661 TCTTGTTCCT CCTCAGAGTC GCTGCTGGTG GTGGGCGGTG TCTCTCATGG AGCACCAGGG   721 GCTCGGGGCT GCCCTGGCGG GGAGGACTCC GTCGAGGGAG AGCAGAAGAT CCGAGACGAG   781 AAGAAGCGCT GGGAGTTCTT GCGAGGCGCA GACTTGGGGC GAGCTTGCTT GTCGTTGAAG   841 GTAAGGAGAC CATCGGAGGG GTCGATGCAC TTCTGGAGCG GCGGCGCCTC AATTCCTTGC   901 AAGTAACAAG CCTGGAGGTT GAACCAACGC TGTGGCCCCG GGCGGGATCC GCTTGCTGTG 961 TTCTTGGCGC AACTGTAGAG CACCTTCTGG TACAGACTTG GCGGCGAGAC GTTACA //
Primer: IRES-F Sequence file: RDB12911_A4Jfc.seq
>12911_12911_A4Jf_IRES_F_1_G04_19.ab1     1 GCAGCTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG    61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG   121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC   181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG   241 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC   301 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG   361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC   421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG   481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC   541 AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC   601 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC   661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC   721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC   781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCAA GGCCTCTCGA   841 GCCTCTAGAG TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAT   901 TTGTGAAAGA TTGACTGGTA TTCTACTATG TGCTCCTTTT ACGCTATGTG GATACGCTGC   961 TTTATGCCTT GTATCATGCT ATGCTCCCGT ATGCTTCATT TCTCTTCTGA TAATTCTTGC  1021 TGCTGTCTCT TTATGAGAGT GTGTCGTGGT CAAGCACGTG CCTGTGGCAC TGGTTGCTGA 1081 CGCACCCACT GTGGCATGCA CACTGTCAGT CTTCGACTCG CATC //
Primer: CMV-Forward Sequence file: RDB12911_A4Jfd.seq
>12911_12911_A4Jf_CMV_Forward_1_E04_13.ab1     1 TGTCACTCCG CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC   121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA   181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG   241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC   301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG   361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG   421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT   481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC   541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC   601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA   661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT   721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA   781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA   841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC   901 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG   961 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG  1021 GGTATAGTGC AGCAGCAGAA CAATTTGCTG AGGCTATTGA GGCGCAACAG CATCTGTGCA  1081 CTCACAGTCT GGGGCAATCA GCAGCTCAGG CAGATCTGAC CTGGTGGAAA GAAATACCCT 1141 TAAAGGAATT CCA //
Primer: BGH_rev Sequence file: RDB12911_A4Jfe.seq
>12911_12911_A4Jf_BGH_rev_1_H04_22.ab1     1 CGTAATACGG GTCTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG    61 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT   121 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT   181 AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG   241 CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC   301 ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA   361 ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT   421 GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA   481 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG   541 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA   601 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT   661 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAG   721 GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC GAAGACGCGG AAGAGGCCGC   781 AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGAT TGAGGGCCGA AGGGACGTAG   841 CAGAAGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG   901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC   961 AGCGGGCAAG GCAGGCGGCG ATGAGTTCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAAG  1021 TCCCGGGAAG GAGCTGACAG GTGGTGGCAA TGCCTCCAAC CAGTGGGGGT GCGTCAGCAA  1081 ACACAGTGCA CCACCACGGC CACGTGTCTG ACAACGGCCA CAACTTCTCA TAAGAGACAG 1141 CCACAAGATT ATCCAGAGAA AATTGAAGGC CATACCGGAA GCCATTAG //
Primer: SV40pro_F Sequence file: RDB12911_A4Jff.seq
>12911_12911_A4Jf_SV40pro_F_1_A05_02.ab1     1 CCCCGTCCTT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT   601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA   661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG   721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA   781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA   841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT   901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT   961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA  1021 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGCGCTCTTC CGCTTTCCTC  1081 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCCGTATCAG CTTCACTTCA 1141 AGCCGGTATT ACGCTTATCC CACAGAATCA ATGGGGATAT AACCGCCCAA G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl