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pDR274 CiHox5Sg

Plasmid clone for in vitro transcription of sgRNA targeting Ciona intestinalis Hox5 gene by Cas9.

Catalog number RDB12998
Resource name pDR274 CiHox5Sg
Clone info. Plasmid clone for in vitro transcription of sgRNA targeting Ciona intestinalis Hox5 gene by Cas9.
Vector backbone pDR274 ()
Size of vector backbone 2.1 kb
Selectable markers Kam^r
Gene/insert name Ciona intestinalis Hox5 genomic DNA
Depositor|Developer Sasakura, Yasunori |
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External Database
Ciona intestinalis Hox5
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Dev Growth Differ., 56, 499-510(2014)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Dev Growth Differ., 56, 499-510(2014))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
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            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB12998 pDR274 CiHox5Sg Under QC test. Please contact us. DNA solution Please contact us.

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pDR274 CiHox5Sg was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12998).

            Reference section:

            Sasaki, H., Yoshida, K., Hozumi, A., Sasakura, Y., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            Featured content

            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Sasaki, H., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715. [link to RRC of NBRP]

            2023.08.17

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