Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCNL-N1

Cyan Nano-lantern to construct fusion proteins to the N-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13325_A5E8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB13325_A5E8a.seq
>13325_13325_A5E8_1_CMV_Forward_G12_21.ab1     1 NNNNNNNNNN NNNNNNNNNN NTGNNGCNNN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG ACTCAGATCT   121 CGAGCTCAAG CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGTCGC   181 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT   241 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC   301 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC   361 CACCCTCGTG ACCACCCTGT CCTGGGGCGT GCAGTGCTTC GCCCGCTACC CCGACCACAT   421 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT   481 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC   541 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG   601 GCACAAGCTG GAGTACAACT ACTTTAGCGA CAACGTCTAT ATCACCGCCG ACAAGCAGAA   661 GAACGGCATC AAGGCCAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT   721 CGCCGANCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA   781 CCACTACCTG ANCACCCAGT CCAAGCTGAN CAAAGACCCC AACGAGAAGC GCGATCACAT   841 GGTCCTGCTG GANTTCGTGA NCGCCGCCGG GGGTNNCAAG GTGTACGANC CCNANCAGAN   901 GAAGANGANG ATCANCGGCC CCCAGNGGNG GGCCANGNGC AAGCANATGA ACNNGCTNGA   961 NAGCTNNNTN NNNNNCNACN ANNNCGANAA NNCNNGCNNN ANNNNNNCNN NNNNNNTNNC 1021 NNNNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB13325_A5E8b.seq
>13325_13325_B0IiF_1_SV40polyA_R2__D11_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTAANNN NAACAATTGC ATTCNTTTTA TGTTTCAGGT    61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC   121 TGATTATGAT CTAGAGTCGC GGCCGCCTAC TGCTCGTTCT TCAGCACTCT CTCCACGAAG   181 CTCTTGATGT ACTTGCCCAT CTCGTCGGGG GCGTCCTCCT GGAGGAAGTG CAGGCCCTTC   241 ACCTTCACGA ACTCGGTGTT GGGGAACTTC TTGGCGCCCT CCACGATGGC GTTGCTGAAG   301 AAGCCGGGGT CGCCCTCGAT GAACAGCTTG GGCAGGTCGT CGCTGGCTCT CAGGTAGGCG   361 TTGTAGTTTC TCACGATCTG CACCACGTCG GGCTTGCCGC CCTTCACCAG GGGGATCTCT   421 CTGGGCCAGC TCAGGGTGGG TCTTCTCACC TCGCCCTTCT CCTTGAAGGG CTCCAGGTAG   481 GCGGCGAACT CCTCGGGCTC CAGCTTTCTC ATGATCTTGC TGGGCAGCAC GGTCTCCACG   541 AAGAAGTTGT TCTCCAGCAC CATCTTCTCG CCCTCCTCGC TCTTGATCAG GGCGATGTCC   601 TCCTCGATGT CTGGCCACTC GTCCCAGCTC TCGATCACGT CCACCACGCT CTCCATGTGC   661 ACGATGGCCT TGATCCTGTC CTGGTGCTCG TAGGCGTAGT GGAAGGCCAG GGCGGCGCCC   721 CAGTCGTGGC CCACGAAGAT GATCTTCTTG GGCANGTTCA GAAGCTCGAA CCAGGCGGTC   781 AGGTACTTGT AGTGGTCCAG CAGCCTGTAG CTGCCGTTGC CGCTCTTGCC GCTCTTGCCC   841 NTGCCGATCA NATCNGGGAN GATGCACCTG GCCNCGGGCT CGATGTGGGG CACCACGNGC   901 CTCNNCAGGN AGCNGCNNNN GGCGNNNCCG TGCNNNAANA TCACGGNNTT CTCNGNGTGC 961 TNCTCGCNGN CNNANNNNTT GANGNNNCTN NNCNNNNNNN NNN //
Primer: mTurquoise2-M_F (Pr0577) Region: insert mid Sequence file: RDB13325_A5E8c.seq
>13325_13325_B0IiF_1_mTurquoise2-M_F_H10_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NGCANNACAC CCCCATCGGC GNNGGCCCCG    61 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCAA GCTGAGCAAA GACCCCAACG   121 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGGGT ACCAAGGTGT   181 ACGACCCCGA GCAGAGGAAG AGGATGATCA CCGGCCCCCA GTGGTGGGCC AGGTGCAAGC   241 AGATGAACGT GCTGGACAGC TTCATCAACT ACTACGACAG CGAGAAGCAC GCCGAGAACG   301 CCGTGATCTT CCTGCACGGC AACGCCACTA GCAGCTACCT GTGGAGGCAC GTGGTGCCCC   361 ACATCGAGCC CGTGGCCAGG TGCATCATCC CCGATCTGAT CGGCATGGGC AAGAGCGGCA   421 AGAGCGGCAA CGGCAGCTAC AGGCTGCTGG ACCACTACAA GTACCTGACC GCCTGGTTCG   481 AGCTTCTGAA CCTGCCCAAG AAGATCATCT TCGTGGGCCA CGACTGGGGC GCCGCCCTGG   541 CCTTCCACTA CGCCTACGAG CACCAGGACA GGATCAAGGC CATCGTGCAC ATGGAGAGCG   601 TGGTGGACGT GATCGAGAGC TGGGACGAGT GGCCAGACAT CGAGGAGGAC ATCGCCCTGN   661 TCAAGAGCGA GGAGGGCGAG AAGATGGTGC TGGANAACAA CTTCTTCGTG GANACCGTGC   721 TGCCCAGCAA GATCATGAGA AAGCTGGAGC CCGANGANTT CGCCGCCTAC CNGGAGCCNT   781 TCAAGGAGAA NNGNCGAGGT GNNAANNACC CACCCTGANC TGGCCNNNAN NNNNNCCCCC   841 NGGNNNNNNG GCGGNNAGCC CNANNNGGNG NANATCGNGA GAAACTACNA NNNNNANNNN 901 NNNNNCNGCG ACNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: NeoR_KanR Sequence file: RDB13325_A5E8d.seq
>13325_13325_B0IiF_1_SV40pro_ori_F_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNCNNNNN NNGCCTCTGN NCTANTCNNG AANTAGTGNN GAGGCTTTTT    61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA   121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT   181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC   241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG   301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG   361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC   421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC   481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG   541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC   601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG   661 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC   721 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGNTGTGGC GGANCGCTAT CNNNCATAGC   781 GTTGGCTACC CNNGATATTG CTGAAGANCT TGGCGGCNAA NNGGGCTGAN NGCTNCCTCN   841 NGCTTTACGG TATCNNCNNC TCCNNATTCG CANNNNNTCG NCNTTCTATC NNCNTNNNTN 901 NANNANTTNN NNTGANNNNN NNTNNNGGNN NTNNNNANNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl