Please review the QC test results indicated by icon below as well as clone information before placing your order.

pONL-N1_ITPKA

Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13349_A5E8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(r ITPKA,hmKO) Sequence file: RDB13349_A5E8a.seq
>13349_13349_A5E8_3_CMV_Forward_D02_11.ab1     1 NNNNNNNNNN NCNNTTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC   121 AAGCTTATGG GCATGGCGCG ACCACGGGGC GCGGGGCCCT GCAGCCCCGG GTTGGAGCGG   181 GCTCCGCGCC GGAGCGTCGG GGAGCTGCGC CTGCTCTTCG AAGCGCGCTG CGCCGCAGTC   241 GCCGCCGCAG CGGCCGCAGG GTCGGATCCA CCGGTCGCCA CCATGGTGAG CGTGATCAAG   301 CCCGAGATGA AGATGAGGTA CTACATGGAC GGCTCCGTCA ATGGGCATGA GTTCACAATC   361 GAGGGTGAGG GCACAGGCAG ACCTTACGAG GGACATCAGG AGATGACACT GCGCGTCACA   421 ATGGCCGAGG GCGGGCCAAT GCCTTTCGCC TTCGACCTGG TGTCCCACGT GTTCTGTTAC   481 GGCCACAGAG TTTTTACTAA ATATCCAGAA NAGATCCCAN ACTATTTCAA GCAGGCCTTT 541 CCTGAGGNNN NNNNNNN //
Primer: Rluc8-M_F2 (Pr1033) Region: insert 3'(Rluc8.6-535) Sequence file: RDB13349_A5E8b.seq
>13349_13349_-B1G9F_Rluc8-M_F2_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG GGNNCGCTCT GGCCTTTCNC TACGCCTACG AGCACCAAGA    61 CAGGATCAAG GCCATCGTCC ATATGGAGAG TGTCGTGGAC GTGATCGAGT CCTGGATGGG   121 GTGGCCTGAC ATCGAGGAGG AGCTGGCCCT GATCAAGAGC GAAGAGGGCG AGAAAATGGT   181 GCTTGAGAAT AACTTCTTCG TCGAGACCCT GTTGCCAAGC AAGATCATGC GGAAACTGGA   241 GCCTGAGGAG TTCGCTGCCT ACCTGGAGCC ATTCAAGGAG AAGGGCGAGG TTAGACGGCC   301 TACCCTCTCC TGGCCTCGCG AGATCCCTCT CGTTAAGGGA GGCAAGCCCG ACGTCGTCCA   361 GATTGTCCGC AACTACAACG CCTACCTTCG GGCCAGCGAC GATCTGCCTA AGCTGTTCAT   421 CGAGTCCGAC CCTGGGTTCT TTTCCAACGC TATTGTCGAG GGAGCTAAGA AGTTCCCTAA   481 CACCGAGTTC GTGAAGGTGA AGGGCCTCCA CTTCCTCCAG GAGGACGCTC CAGATGAAAT   541 GGGTAAGTAC ATCAAGAGCT TCGTGGAGCG CGTGCTGAAG AACGAGCAGT AGGCGGCCGC   601 GACTCTAGAT CATAATCNGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC   661 TCCCNCNCNT CCCCCNGNNN CNGANNATAA AATGNATGNN ATTGNTGNTG NNNNCTNGNT 721 NATNNNNNCT NNNNNNNGNN NNNNNNNN //
Primer: hRluc_N_rev (Pr0567) Region: insert mid(Rluc8.6-535,hmKO) Sequence file: RDB13349_A5E8c.seq
>13349_13349_B1GfF_3_hRluc_N_rev_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNTTNGCT TGNNGCGAGC CCNCCACTGA GGCCCAGTGA TCATGCGTTT    61 GCGTTGCTCG GGGTCGTACA CCTTGGTACC GGAGTGGGCC ACGGCGTCCT CCACCTGCTC   121 GGTGATGTTG CCCTCGGTCT TCCTCACCAG CCTGTGGCCG ATGTAGTGGT CGCCGGGCAT   181 CTCCAGGATC TCCTTGGCGG CCTTGTAGGT GGTCTTCATC TGGCACTTGT GGTTGCCGCC   241 GCCCTCCAGC TTCAGGTACA TGGTCACGTC GCCCTTCAGC ACGCCGTCGC TGGCGGTGAT   301 CTTCTCGGTG GAGGGCTCCC AGTCCACGCT CTGGTTCTGC ATGATGGGGC CGTCGGCGGG   361 GAAGTTCACG CCGGTGAACT TGGACTTGTG GTAGAAGGTG TTGCCCCTCA GGCTGATGTG   421 GGCGCTCACG GAGGCGGAGC CGCCGTCCTC GAACTCCAGG GACCTCTCCC AGGACAGGCC   481 CTCAGGAAAG GCCTGCTTGA AATAGTCTGG GATCTCTTCT GGATATTTAG TAAAAACTCT   541 GTGGCCGTAA CAGAACACGT GGGANACCAG GTCGAANGCG AAAGGCATTG GCCCGCCCTC   601 NGCCNTTGTG ACGCGCAGTG TCNTCTCCTG ATGTNCCTCN TAANGNCNGN CTGTGCCNTC   661 NCCCTCNANT GNGAACNCNT GCCCATTGAN NNNNNTCNNT GNNNNANNTN NTCNTNNTCT 721 NNGGCNNGNT NNCNCTNNNN NNGNNNNNNN NNN //
Primer: mKO2-M_F (Pr0578) Region: insert mid(hmKO,Rluc8.6-535) Sequence file: RDB13349_A5E8d.seq
>13349_13349_B1GfF_3_mKO2-M_F_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNGCNNGANG AAGANCACCT ACNAGGCCGC CAAGGAGATC    61 CTGGAGATGC CCGGCGACCA CTACATCGGC CACAGGCTGG TGAGGAAGAC CGAGGGCAAC   121 ATCACCGAGC AGGTGGAGGA CGCCGTGGCC CACTCCGGTA CCAAGGTGTA CGACCCCGAG   181 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG   241 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT   301 CTGCATGGTA ACGCTACCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC   361 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT   421 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC   481 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGA GCGCTCTGGC CTTTCACTAC   541 GCCTACGAGC ACCAAGACAG GATCAAGGCC ATCGTCCATA TGGAGAGTGT CGTGGANGTG   601 ATCGANTCCT GNATGGGGTG GCCTGACNTC GANGANNANC TGGNCCNGAT CAAGAGCGAA 661 NANGGNGANA AAATGGTGCT TGANAANNAC TNCNNCNNCN ANANCNNNNN NNNNN //
Primer: RLuc8-m1dN3-C_F (Pr0265) Region: insert 3'(Rluc8.6-535),SV40 pA,f1 ori Sequence file: RDB13349_A5E8e.seq
>13349_13349_B1GfF_3_Rluc8-m1dN3-c_F_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCTT CGTGGANCGC GTGCTGAAGA ACGAGCAGTA GGCGGCCGCG    61 ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT   121 CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT   181 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC   241 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAGGCGT   301 AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT   361 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA   421 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA   481 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT   541 AATCAAGTTT TTTGGGGTCG AGGTGNCGTA AAGCACNAAA TCGGAACCCT AAAGGGAGCC   601 CCCGATTTAN AGCTTGACGG NGAAAGCCNG CGANNGTGNC GANAAANGAA NGGAANAAAG   661 NNNAAGGANC GGGCGCTNNG GCNCNGGCNA GNGNANCGGT CANNCTGCNC NNANCNNCCN 721 NNCCNNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori,NeoR_KanR Sequence file: RDB13349_A5E8f.seq
>13349_13349_B1G9F_SV40pro_ori_F_H01_22_ABI24.ab1     1 NNNNNNNNNN NNGCCGCCTC NGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT    61 GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT   121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA   181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA   241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA   301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA   361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT   421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG   481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA   541 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA   601 TCNNNNNCTC GCGCCNGCCG AACTGTTCGC NNGGCTCNNN GCGANCATNN NNNANGGCGA   661 GGNNCTCGTC NTGNNCCANG NNNATNCNTG CTTNNCGAAN ATCATGGNNG AAANNGGNNN 721 CNTTNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl