Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEMHE-mBubR1-WT

Plasmid for in vitro transcription of mouse Bub1b in mouse oocyte.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13513_A6Aep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB13513_A5Isa.seq
>D02675A5-A5Is_4_T7_20mer_B02_05.ab1     1 TACCCCGGTA CCAGCTTGCT TGTTCTTTTT GCAGAAGCTC AGAATAAACG CTCAACTTTG    61 GCAGATCAAT TCCCCGGTGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA   121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA   181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT   241 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA   301 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC   361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA   421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA   481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA   541 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA   601 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC   661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC   721 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTTAAGGAGT TCGTGACCGC   781 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAC TCAGATCTCG AGCTCAAGCT   841 TCGAATTCTG CAGTCGACGA TGGCGGAGGC GAGTGAAGCC ATGTGCCTGG AGGGAGCAGA   901 GTGGGAGCTG AGTAAAGAAA ACATACAGCC CTTTACGGCA CGGGCGGGTC ATGTCCACAC   961 TTCAGGAGCT TTGGGCAAGC AGAGTCAGCC CGGCCACACT GCTCTGCAGC AGCAGAAACC  1021 GGGCAATTTT GATCTGAAAT CCGCTTTTAC TCTGAGATGA ACCCTCTGGT ATGTGGACCG 1081 ATATATATGG GACAGAAACG GAACTTAA //
Primer: mEGFP-down2 Sequence file: RDB13513_A5Isb.seq
>D02675A5_A5Is_4_mEGFP-down2_E12_15.ab1     1 CTGAATTTGC AGTCGACGAT GGCGGAGGCG AGTGAAGCCA TGTGCCTGGA GGGAGCAGAG    61 TGGGAGCTGA GTAAAGAAAA CATACAGCCC TTACGGCACG GGCGGGTCAT GTCCACACTT   121 CAGGGAGCTT TGGCAAAGCA AGAGTCAGCC GGCCACACTG CTCTGCAGCA GCAGAAACGG   181 GCATTTGAAT CTGAAATCCG CTTTTACTCT GGAGATGACC CTCTGGATGT GTGGGACAGA   241 TATATTAATT GGACAGAACA GAACTACCCT CAAGGGGGGA AAGAGAGTAA CATGTCAGCG   301 TTAGTGGAGA GAGCGATAGA AGCACTCCAA GGAGAGACGC GCTATTATAA TGACCCCCGC   361 TTTCTCAGTC TCTGGATCAA ATTGGGACAT TTGTGCAATG AACCTTTGGA TATGTACAGC   421 TATTTACAAA GCCAAGGAAT TGGCGTTTCC CTTGCCCAGT TCTATATTTC ATGGGCTGAA   481 GAATACGAAG CTAGAGAAAA TTTCAAGAAA GCGGACATAA TATTCCAGGA AGGGATTGAA   541 CGCAAGGCTG AGCCCCTGGA CAGACTGCAG TCCCAGCACA GACAGTTCCA GTCTCGAGTG   601 TCGCGACAAG CTTTCTTGGC CCTTGGGAAT GAAGAGGAGA GCTTTGGAGC CTTCTGAACC   661 ACAGAGAGCT CGCTAGCTGA GCTGAAGCAA AGGAAGAAGA TGGCCAGAGC GCCATCAGCG   721 TGTCGAGGTG CTCTGAAAGC TCCAGTCGAG CGAAGGTCTA AATGCAGTTC CCCAGCAGTA   781 CACGGTAATC GCAGATCACC GTTTTGATGA ATGGCGAAAC CCGGCTCTAA CGATTATAGC   841 CTTGTACCCA CCATGGATGC ACCCTTGTGC CAGGCAAGGA AAGACTCACA GTCTAGGGAA   901 CAATGGCCGG GAACAGCCTA GAATCAGCCT GGGAGCGAAT CCAGGTCCAG TACGATGAAG   961 GCCCCAACTG AACCTGATGC TATACCGTTC CCGACCGGGA CGACCTTGGA GTCACTCCGT 1021 GAGGAGTCGG AAACT //
Primer: RDB13509#1 Sequence file: RDB13513_A5Isc.seq
>D02675A5-A5Is_4_RDB13509#1_D02_11.ab1     1 CGGGCCTTTT GTAGTGTAAG TTGGTATTAT GTAGCTTAGA GACTCCATTC GGGTGTTCTT    61 GAGGCTGGTT TAGTGGTAAC CAGATCAAGC TATCATTTAT CTAGAGCGGC CGCTTAGGCA   121 GGGCTCTGGG AGCCGCCTGG CTGCTTGTCT TGCTGAGTGA GCAAAGCTTC CGGGCTGATT   181 GTCTTCCCCA GCTTCCACAG GGCTCTGTTC AGGTGGCTAT GGAATGTGGC ATCAAAAGCC   241 CCACCCATTT CTGCTGCCAG CTCCCCCAGA ACAGACACTG TGGACTTGTC ACTGGCATTC   301 AGAATCCGCA CAAAGAATTT ATTCCACAAT TCACTGTCTT TTAGCTCAGA GGTGCTCTGG   361 CTAAGTTTCC AGAGGAGTCC ATCCCAGAAG ACATGGAGGT GCTCCTTGAA CAGGAGTAAG   421 TGCGCTAGGT CTGCTATACC CAACAGATCT ACATGGTAGG GAGAAGAACA GTTAGCCAGG   481 ATCTTTTGTC CTTCCAGGAT CTGTGCAGTC CGAAAGCCAC TATAGGCAAA CGCATCCAGC   541 TGCACCCTCA GGTCAACACT GTAGGAGAAG TCCATGATCC TCACAGCGTG ATCGTCCTTA   601 TTTACATAGT CATAGGGGTC GTGGATTCTG TTTCGTAGGA TCAGACTCCG TGGACTCAAG   661 TCTCCGTGTA CTATTTCAGC TCTGTGTAGC TTCTCCACGA TTGTCAAGAG GTTGTAAATA   721 ATCAACACTA TTATTTCATG AGTAACAAAT TCGCTGTGTT GGAGAAGATT CTGAAGGGTG   781 GAGCAGTTTA TATACTGGTA CCAAACAACA TGGCCATCTT GGTACTGACA GCAGCTGCAA   841 AGCTGGTCAT AGTCCTCATT CAGACGCTCC TTCAACTTGA GGTTGATATA AAAAATCCCA   901 CGGGATAGGC TGAGATGATG CCTTTATCAT GGGTTAGCTC TGCAGAGCTT CTTTGGTGCC   961 ACCCCAGAAT AACCCTGTAA TCGTCACATG TTTAGTGCTC TTGCTTGATG ACGTTAATCT  1021 CAGACCCTAA CTCAATCTCG ACCCTATTTC AAGCACAGCA TCGGTTCGGA TCATCCACAG 1081 AAAACTTTCG CAAAAGTCAA CT //
Primer: RDB13513#1 Sequence file: RDB13513_A5Isd.seq
>D02675A5-A5Is_4_RDB13513#1_E02_14.ab1     1 CCGTTACCGA GAGGGAATGT TTGCAGGAAA GAGGATCTGG ATGGGAAAGG CCGGCCGAGG    61 CTCATCACAC TGTTCATCAC CAGGCCCTCA TCATAAAGAA ACTGAGCCCA ATTATTGAAG   121 AGAGCCGTGA GGCCACCCAC TCATCTGGCT TCTCCAGGTC TTCTTCCTCA GCTCCCAGTA   181 CATCCTCCAT CAAAGGCTTT CAGCTTCTGG AAAAGCTGGA GCTGACTAAT GACGGGGCAG   241 AAGATGCTAT TCAGTCACCC TGGTGTTCAC AGTATCGCCT ACAACTGTTA AAATCCCTAC   301 TAGAATTAAG TGCTTTTGCG GAGTTTTCTG TGGAAGACCG ACCGATGCCT GTGCTGGAAA   361 TAGGGAAGGA GATTGAGTTA GGTCCTGAGG ATTACGTCAT CAAGCAAGAG CACCTAACAT   421 GTGACGATTA CAGGTTATTC TGGGTGGCAC CAAGAAGCTC TGCAGAGCTA ACCATGATAA   481 AGGCATCATC TCAGCCTATC CCGTGGGATT TTTATATCAA CCTCAAGTTG AAGGAGCGTC   541 TGAATGAGGA CTATGACCAG CTTTGCAGCT GCTGTCAGTA CCAAGATGGC CATGTTGTTT   601 GGTACCAGTA TATAAACTGC TCCACCCTTC AGAATCTTCT CCAACACAGC GAATTTGTTA   661 CTCATGAAAT AATAGTGTTG ATTATTTACA ACCTCTTGAC AATCGTGGAG AAGCTACACA   721 GAGCTGAAAA TAGTACACGG AGACTTGAGT CCACGGAGTC TGATCCTACG AAACAGAATC   781 CACGACCCCT ATGACTATGT AAATAAAGGA CGATCACGCT GTGAGGATCA TGGACTTCTC   841 CTACAGTGTT GACCCTGAGG GTGCAGCTGG ATGCGTTTGC CTATAGTGGC TTTCGGACTT   901 GCACAGATCC TGGAAAGGAC AAAAGATCCC TGGCTAACTG TTCTTCCTCC CTACCAATGT   961 AGATCTGTTT GGGGTATAGC CAGACCCTAG CCGCAACTTA CTTCCCTGTT CCAAGGGAGC  1021 CACCCTTCCA TGTTCTTTCC TTGGGAATGG ACCTTCCTTC TGGGAAACCT TTAGCCCAGA  1081 AGCCAACCCT TCTGAAGCTT AAAAAGACAC AGTTGAAAAT TTGTGGGAAT TAAATTCCTT 1141 TTGTTGCCGA TATTCTGAAA TGACCAAGGT GGGAAACAAG AAGATTTTCC CA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl