Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEMHE-mEGFP-INCENP

Plasmid for in vitro transcription of human INCENP in mouse oocyte.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13519_B0Jup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574) Region: insert mid (mEGFP 3', INCENP 5') Sequence file: RDB13519_B0Jua.seq
>13519_13519_B0Ju_1_mVenus-M_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNANNNGGN NTCNNGGTGN NCTTCNNGAN CCGCCNCNAC ATCGAGGACG    61 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC   121 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA   181 AGCGCGATCA CATGGTCCTT AAGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG   241 ACGAGCTGTA CAAGTACTCA GATCTCGAGC TCAAGCTTCG AATTCTGCAG TCGACGGCCA   301 CCATGGGGAC GACGGCCCCA GGGCCCATTC ACCTGCTGGA GCTATGTGAC CAGAAGCTCA   361 TGGAGTTTCT CTGCAACATG GATAATAAGG ACTTGGTGTG GCTTGAGGAG ATCCAAGAGG   421 AGGCCGAGCG CATGTTCACC AGAGAATTCA GCAAAGAGCC AGAGCTGATG CCCAAAACAC   481 CTTCTCAGAA GAACCGACGG AAGAAGAGAC GGATTTCTTA TGTTCAGGAT GAAAACAGAG   541 ATCCCATCAG GAGAAGGTTA TCCCGCAGAA AGTCTCGGAG CAGCCAGCTG AGCTCCCGAC   601 GCCTCCGCAG CAAGGACAGT GTAGAGAAGC TGGCTACAGT GGTCGGGGAG AACGGCTCCG   661 TTCTGCGGCG TGTGACCCGT GCTGCGGCTG CAGCTGCCGC GGCTACCATG GCATTGGCTG   721 CACCTTCTTC ACCCACCCCT GANTCTCCCA CGATGCTGAC TAANAAGCCC GANGATAACC   781 ACACCCAGNG CCAGCTGGTG CCTGTGGNGG AGATCGGNAT CAGTGAGCGN CNGAATGCTG   841 ANCANCATGT CACCCAGCTC ATGTCCNCCG ANCCTCNGCC CCGCANNCTG NCCCCGANTC   901 CNGCTTCNNN NNNNGCTNCN ANCNTCCCAG GGNATCCCGA NNTCNNANGA NGNNNCNNNN   961 NCNTANGNNG NNNAANGNCN NNNANACTNN NNNCCANNNN NNNGANNNNC NNNNNNNNNN //
Primer: RDB13509#1 (Pr0307) Region: insert mid (Xenopus globin 3'-UTR, INCENP 3') Sequence file: RDB13519_B0Jub.seq
>13519_13519_B0Ju_1_RDB13509#1_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNANNNGT NNNNNGGTAT TATGTAGCTT AGAGACTCCA TTCGGGTGTT    61 CTTGAGGCTG GTTTAGTGGT AACCAGATCA AGCTATCATT TATCTAGAGC GGCCGCTTTA   121 CTTGTACAGG ATCCGCCTAG TGCTTCTTCA GGCTGTAGGC CAGGCTGCTG GGGACCCTGG   181 CGCCCTGCAG GGGCGGTGAG TTCCAGACAG CAGAGCTGGT GCGCTTGTGA TAGCGGGGCT   241 TGCTCTTCTT GAAGATATCC TCCAAGTCCA GTGGGAGAAT GGTTCCAAAG AGCTCCAGAA   301 GGTTCGGTGG GTGGTAGTAC TGGTGAATGA TAGCCTGGCT GAGCGGGGTG CCTCGGGCCC   361 AGGTGGGGAT GGGCTTCCGG GGATGGGCCT CATCATCGGT GGAGTCGTCG CTATTCAGAT   421 CCATCCCGTA GTTATCTGGG TTGATCTTGG GAGGGGCCCT GTGCCCTTGC GGAGTCATCT   481 GATATGAGGT ACAAGCTGGA GACTGCACGT CCACAGTCAC ATTCAGGGCC TTGCTGGCCC   541 CTGCTGCCTC CTTGGCTTTC TTCTCTTGCT CCTCCTGCAG CTGCCGCTCA GCCAGACGCT   601 GCTGCTCTTC CTTCTTCTTC TTCTCCTCCA GTTCCCTCTG CAGCTGCTCC TTCTGCAGCC   661 GCANGGCCTT CTCCCGCTCC TGCAGCTNCC TCTCNGNCCT NNNCCGCTCC NGNTNCCGNN 721 NANNCNNNNN TCNNNNNNNN NN //
Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (Xenopus globin 5'-UTR, mEGFP 5') Sequence file: RDB13519_B0Juc.seq
>13519_13519_B0Ju_1_HB-NOS_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNGNGNNN NNTTGGNNNN NCCNGGGTTT TCCCAGTCNC GACGTTGTAA    61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTAATTC GAGCTCGGTA   121 CCCAGCTTGC TTGTTCTTTT TGCAGAAGCT CAGAATAAAC GCTCAACTTT GGCAGATCAA   181 TTCCCCGGTG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC   241 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT   301 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC   361 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA   421 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC   481 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG   541 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA   601 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA   661 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA   721 CAACATCGAG GANGGCAGCG TGCAGCTCGC CGACCACTAC NAGCAGANCA CCCCCATCGG   781 CGACNGCCCC GTGCTGCTGC CCGACNACCA CTACCTGANC ACCCAGTCCG CCCTGANCAA   841 AGACCCCAAC GAGAANCGCG ATNACATGGT NCTTAAGGAG TTCGTGANNG CCGCCGGGNT   901 CACTCTCGGC ANGGNNGANC NGTACNAGTA CTCANATCTC GAGCTNNNGC TTCNNNNTCN   961 GCANNCNANN NNCNNCNNNG NNNNNGANNG NNNNAGGGNC CATNNNNCNN GCNNNNNNNN 1021 NN //
Primer: SV40pA3'element Reverse (Pr0108) Region: SP6 pro Sequence file: RDB13519_B0Jud.seq
>13519_13519_B0Ju_1_SV40pA3'elementReverse_H03_24_ABI24.ab1     1 NNNNNNNGNN NNNNNNNNGN NNNNGCCNGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT    61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT   121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG   181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG   241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC   301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT   361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT   421 GACCATGATT ACGCCAAGCT ATTTAGGTGA CACTATAGAA TACTCAAGCT AGATTAATTA 481 ATTTAAATCG CCGGCGGCGC GCCTGCAGGG GGGGGGGGGG NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

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