Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV_Tag4_hEGFR WT

Expression vector of FLAG-tagged human EGFR in mammalian cells.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Hiragun, T., Biosens. Bioelectron. 32 (1): 202-207, 2012)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hiragun, T., Biosens. Bioelectron. 32 (1): 202-207, 2012)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13714_A9H2p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, insert 5' Sequence file: RDB13714_A9H2a.seq
>13714_13714_A9H2_2_CMV-Forward_A07_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TTNNNGCANT GGGCGGTANG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGATTACGCC AAGCTCGAAA   121 TTAACCCTCA CTAAAGGGAA CAAAAGCTGG AGCTCCACCG CGGTGGCGGC CGCTCTAGCC   181 CGGGCGGATC CCCCGGGCTG CAGGAATTCG ATATCGCTCT TCGGGGAGCA GCGATGCGAC   241 CCTCCGGGAC GGCCGGGGCA GCGCTCCTGG CGCTGCTGGC TGCGCTCTGC CCGGCGAGTC   301 GGGCTCTGGA GGAAAAGAAA GTTTGCCAAG GCACGAGTAA CAAGCTCACG CAGTTGGGCA   361 CTTTTGAAGA TCATTTTCTC AGCCTCCAGA GGATGTTCAA TAACTGTGAG GTGGTCCTTG   421 GGAATTTGGA AATTACCTAT GTGCAGAGGA ATTATGATCT TTCCTTCTTA AAGACCATCC   481 AGGAGGTGGC TGGTTATGTC CTCATTGCCC TCAACACAGT GGAGCGAATT CCTTTGGAAA   541 ACCTGCAGAT CATCAGAGGA AATATGTACT ACGAAAATTC CTATGCCTTA GCAGTCTTAT   601 CTAACTATGA TGCAAATAAA ACCGGACTGA AGGAGCTGCC CATGAGAAAT TTACAGGAAA   661 TCCTGNNNGG CGCCGTGCGG TTCAGCAACA ACCCTGCCCT GTGCAACGTG GAGAGCATCC   721 AGTGGCGGGA CATAGTCAGC ANTGACTTTC TCAGCAACAT GTCGATGGAC TTCCANAACC   781 ACCTGGGCAG CTGCCAAAAG TGTGATCCAA GCTGTCNCAA TNGGAGCTGC TGGGGTGCNN   841 NANAGGANAA NTGNNNNNNA ACTGACNAAA ATCATCTGTG CCCNNCAGNG CTNCGGGNGC   901 TGCCNNGGNN AAGNNCCCCC AGNNACNNCT NNNANNANCN ANNGTGCNNG CNNNNNNCNN 961 NNNNNNNN //
Primer: T7_20bp Region: FLAG, insert 3' Sequence file: RDB13714_A9H2b.seq
>13714_13714_A9H2_2_T7_20bp_G08_20_ABI24.ab1     1 NNNNNNNNNN NGNNNNNNNN TTNNNNAAGN NACCGGGCCC TACTTATCGT CGTCATCCTT    61 GTAATCCTCG AGTGCTCCAA TAAATTCACT GCTTTGTGGC GCGACCCTTA GGTATTCTGC   121 ATTTTCAGCT GTGGAGCCCT TAAAGATGCC ATTTGGCTTG GCTTCCTTGG GAAAGAAGTC   181 CTGCTGGTAG TCAGGGTTGT CCAGGCTAAT TTGGTGGCTG CCTTTCTGGG CCCAGTGGGC   241 AGGGCTGTCG AATGTGCTGT TGACACAGGT GGGCTGGACA GTGTTGAGAT ACTCGGGGTT   301 GCCCACTGCA GTGCTGTGGG GGTCCTGGTA GTGTGGGTCT CTGCTGGGCG CGGGGTTCAG   361 AGGCTGATTG TGATAGACAG GATTCTGCAC AGAGCCAGCG GGCCTTTTGG GAACGGACTG   421 GTTTATGTAT TCAGGCACTG GGAGGAAGGT GTCGTCTATG CTGTCCTCAG TCAAGGCGCC   481 TGTGGGGTCT GAGCTGTATC GCTGCAAGAA GCTGTCTTCC TTGATGGGAC AGCTTTGCAG   541 CCCATTTCTA TCAATGCAAG CCACGGTGGA ATTGTTGCTG GTTGCACTCA GAGAGCTCAG   601 GAGGGGAGTC CGTGACGTGG AGGGGCTGCT GAAGAAGCCC TGCTGTGGGA TGAGGTACTC   661 GTCGGCATCC ACCACGTCGT CCATGTCTTC TTCATCCATC AGGGCACGGT AGAAGTTGGA   721 GTCTGTAGGA NTTGGCAAAT GCATTCTTTC ATCCCCCTGA ATGACAAGGT AGCGCTGGGG   781 GTCTCGGGCC ATTTTGGAGA ANTCGATGAT CAACTCACGG AACTTTGGGC GANTATCTGC   841 GTCTATCATC CAGCACTTGA CCATGATCAT GTAGACATCG ATGGTACNTA TGGGTGGNTG   901 ANGGNNGNGT TCTCCTTTCN CCNNGNNGGA GGANATNNCG CTNGNAGGGN NTNCNNCATA   961 TGGNTNNNNN NCAAAGGNCA TCNNCNCCCA AANNNNNACC CCGTANCTNC NNNNNTNNNN 1021 NNNGNNNGNN NNNNNNNN //
Primer: EGFR-F5 Region: insert middle region Sequence file: RDB13714_A9H2c.seq
>13714_13714_A9H2_2_EGFR-F5_C07_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGNNGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC    61 CTGGGGATCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG   121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA   181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT   241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC   301 GTCGCTATCA AGGAATTAAG AGAAGCAACA TCTCCGAAAG CCAACAAGGA AATCCTCGAT   361 GAAGCCTACG TGATGGCCAG CGTGGACAAC CCCCACGTGT GCCGCCTGCT GGGCATCTGC   421 CTCACCTCCA CCGTGCAACT CATCACGCAG CTCATGCCCT TCGGCTGCCT CCTGGACTAT   481 GTCCGGGAAC ACAAAGACAA TATTGGCTCC CAGTACCTGC TCAACTGGTG TGTGCAGATC   541 GCAAAGGGCA TGAACTACTT GGAGGACCGT CGCTTGGTGC ACCGCGACCT GGCAGCCAGG   601 AACGTACTGG TGAAAACACC GCAGCATGTC AAGATCACAG ATTTTGGGCT GGCCAAACTG   661 CTGGGTGCGG AAGAGAAAGA ATACCATGCA GAAGGAGGCA AAGTGCCTAT CAAGTGGATG   721 GCATTGNAAT CAATTTTACA CAGAATCTAT ACCCACCAGA GTGATGTCTG GAGCTACGGG   781 GTGACCGTTT GGGANTTGAT GANCTTTGGA TCCAAGCCAT ATGACGGAAT NCCTGCCAGC   841 GAGATCTCCT CCATCCTGGA NAAANNAGAN CGCCTCCCTC AGCCACCCAT ATGTACCATC   901 GATGTCTANN TGATCATGGN CAAGTGCTGG NTGATAGACN CNNANNNNNN CCCNAANNTT 961 NCNNGNNNNT NGATCANNGA ANTTNNNNNA AANNGNCCCN NNNNNNNNN //
Primer: SV40pro_F_V2 Region: SV40pro, NeoR_KanR Sequence file: RDB13714_A9H2d.seq
>13714_13714_A9H2_2_SV40proF_V2_H08_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCTT TTGNNAAGNN CGNTCAAGAG ACNGGNTGAG    61 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG   121 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT   181 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC   241 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT   301 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG   361 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG   421 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG   481 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG   541 ATCTGGACGA AGAACATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA   601 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA   661 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC   721 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAACTTGGC GGCGAATGGG   781 CTGANCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTNGCANCGC ATCGCCTTCT   841 ATCGCCTTCT TGACGAGTTC NTCTGAGCGG GANTCTGGGG TTCGAAATGA NCGANCNAGC   901 NACNNCCAAC CTGCCATCAC GAGANTTCNA TTNNNCCGCC NNCNTTCTAT GAAANGNTGG 961 GNNTTNNGNN NNNGTTTTNC NGGNNNNCNN GCNNGNNNGA TNCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl