Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV-Tag2B_ALDH2

Expression vector of FLAG-tagged human ALDH2.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Amanuma, Y., Sci. Rep. 5: 14142, 2015)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Amanuma, Y., Sci. Rep. 5: 14142, 2015)
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13740_A6A5p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMVpro,T3pro,FLAG,insert5 Sequence file: RDB13740_A6A5a.seq
>D02971A1_A6A5_1_CMV_Forward_B05_05.ab1     1 TTGTTCAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GATTACGCCA AGCTCGAAAT   121 TAACCCTCAC TAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC GCCACCATGG   181 ATTACAAGGA TGACGACGAT AAGAGCCCGG GCGGATCCCC CGGGCTGCAG GAATTCATGT   241 TGCGCGCTGC CGCCCGCTTC GGGCCCCGCC TGGGCCGCCG CCTCTTGTCA GCCGCCGCCA   301 CCCAGGCCGT GCCTGCCCCC AACCAGCAGC CCGAGGTCTT CTGCAACCAG ATTTTCATAA   361 ACAATGAATG GCACGATGCC GTCAGCAGGA AAACATTCCC CACCGTCAAT CCGTCCACTG   421 GAGAGGTCAT CTGTCAGGTA GCTGAAGGGG ACAAGGAAGA TGTGGACAAG GCAGTGAAGG   481 CCGCCCGGGC CGCCTTCCAG CTGGGCTCAC CTTGGCGCCG CATGGACGCA TCACACAGGG   541 GCCGGCTGCT GAACCGCCTG GCCGATCTGA TCGAGCGGGA CCGGACCTAC CTGGCGGCCT   601 TGGAGACCCT GGACAATGGC AAGCCCTATG TCATCTCCTA CCTGGTGGAT TTGGACATGG   661 TCCTCAAATG TCTCCGGTAT TATGCCGGCT GGGCTGATAA GTACCACGGG AAAACCATCC   721 CCATTGACGG AGACTTCTTC AGCTACACAC GCCATGAACC TGTGGGGGTG TGCGGGCAGA   781 TCATTCCGTG GAATTTCCCG CTCCTGATGC AAGCATGGAA GCTGGGCCCA GCCTTGGCAA   841 CTGGAAACGT GGTTGTGATG AAGGTAGCTG AGCAGACACC CCTCACCGCC CTCTATGTGG   901 CCAACCTGAT CAAAGGAGGC TGGCTTTCCC CCTGGTGTGG TCAACATTGT GCCTGGATTT   961 GGCCCCACGG CTGGGGCCGC CATTGCCCTC CCATGAGATG TGGACAAAGT GGCATTCACA  1021 GCTCCACTGA GATTGGGCCG CGTATCCAGG TGCTGCTGGG GAGCAGCACT CAGAGAGTGA  1081 CTGCAGCTGG GGGGAGAGCC CACATCATCA TGTCAGATGC CGGAATATGT GAATTGGGGG 1141 CG //
Primer: SV40polyA_R2 Region: SV40p(A),T7pro,insert3' Sequence file: RDB13740_A6A5b.seq
>D02971A1_A6A5_1-SV40polyA_R2_E10_05_ABI08.ab1     1 CATTAAAAGG CTGGCAATAA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA    61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT   121 GGCTGATTAT GATCATGAAC AGACTGTGAG GACTGAGGGG CCTGAAATGA GCCTTGGGAC   181 TGTGAATCTA AAATACACAA ACAATTAGAA TCAGTAGTTT AACACATTAT ACACTTAAAA   241 ATTGGATCTC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGAGTGAATT   301 GTAATACGAC TCACTATAGG GCGAATTGGG TACACTTACC TGGTACCTTA ATTAATTAAG   361 GTACCGGGCC CCCCCTCGAG TTATGAGTTC TTCTGAGGCA CTTTGACTGT GACAGTTTTC   421 ACTTCAGTGT ATGCCTGCAG CCCGTACTCG CCCAACTCCC GGCCACTCCC CGACATCTTG   481 TAGCCACCAA AGGGTGACTG GGCTCCAAAC ACATCATAGC AGTTGACCCA CACAGTGCCC   541 GCCTGGAGGG CCTGGGACAG GTAATTGGCC TTGTCCAAAT CCTTTGTGAA GACAGCTGCG   601 GCCAGCCCGT ACGTGGAATT GTTGGCTCTC CCAACAACCT CCTCTATGGT CTTGAACTTC   661 AGGATCTGCA TCACTGGCCC GAAGATCTCC TCCTTGGCGA TGGTCATGCC ATCCTGCACA   721 TCTCCAAACA CAGTGGGCTG GATGAAGTAA CCACGGTCAG CAGCAATGCC CCCACCACAC   781 AGCAGCTTCG CCCCCTCTTG CTTCCCCGTG TTGATGTAGC CGAGGATCTT CTTAAACTGA   841 GTTTCATCCA CCTGCGGCCC CTGCTCGGTC TTGCTATCAA AGGGGTTCCC GACCACCCGA   901 GACTTGGCCC GGGCAACGCT CCGCTCCACA AACTCATCAT AGATGTCCTC CTGCACGAAA   961 GTCCGGGAGC CGGCACAGCA GCACTGGGCC CTGGTTGAAG AACAGGCGAA GTGGGCCTGT  1021 TCCACGGGCC CAATCCATAT CGGCATCTGA CATGATGATG TGGGGCTCTC CCCCCCAGCT  1081 CCAGTCACTC TTTGAGTTGC TGCTCCAGCA GCAACTTGGA TTACGGGCGG GCCAAATCTC //
Primer: SV40pro_F_V2 Region: SV40pro_ori,NeoR_KanR Sequence file: RDB13740_A6A5c.seq
>D02971A1_A6A5_1_sv40poly_F_v2_D05_11.ab1     1 ACGAGCCTTT TTTTTTGGAG GCCTAGGGCT TTTTGGCAAG ATCGATCAAG AGACAGGATG    61 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT   121 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT   181 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC   241 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC   301 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA   361 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT   421 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA   481 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA   541 TGATCTGGAC GAAGAACATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC   601 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT   661 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGGCGG   721 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAAACTTG GGCGGCGAAT   781 GGGGCTTGAC CCGCTTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGGCAA   841 TCGCCTTCCT ATCGCCTTTC TTGAACGAGT TCCTTCTGAA CGGGACTCTT GGGGGTTCGA   901 AATGAACCGA CCAAACCGAC GCCAAACCTG GCCATCGCGA GATTTCGAAT TCCCCCTCCG   961 CCCTTCTATA AAAGTTTGGG CTTTCGGAAT CTTTTTCCGG GACGCCCGGC TGGGATGATC  1021 CTCCAACGCG GGATCTCCAG GCTGGAATTT CTCCGCCCAC CCCTAGGGGG TAGGCTAACT 1081 GAAACCACGG GAAGGAGAAA AATTACCGGT AAACGGAAAA CTCCGCGTCC TAATGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl