Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK278 (mClover-Hygro)

A plasmid for construction of C-terminal mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering

Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13921_A6B8p1_3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: T7pro,insert5'(mClover(A206K),SV40p(A) Sequence file: RDB13921_A6B8e.seq
>D03070A05_A6B8_1_M13(-40)_B02_05.ab1     1 CCGCCGGTAG CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG ATCCGGTGCA    61 GGCGCCGCTA GCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG   121 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TCCGCGGCGA GGGCGAGGGC   181 GATGCCACCA ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG   241 CCCTGGCCCA CCCTCGTGAC CACCTTCGGC TACGGCGTGG CCTGCTTCAG CCGCTACCCC   301 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG   361 CGCACCATCT CTTTCAAGGA CGACGGTACC TACAAGACCC GCGCCGAGGT GAAGTTCGAG   421 GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC   481 ATCCTGGGGC ACAAGCTGGA GTACAACTTC AACAGCCACA ACGTCTATAT CACGGCCGAC   541 AAGCAGAAGA ACGGCATCAA GGCTAACTTC AAGATCCGCC ACAACGTTGA GGACGGCAGC   601 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG   661 CCCGACAACC ACTACCTGAG CCATCAGTCC AAGCTGAGCA AAGACCCCAA CGAGAAGCGC   721 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TTACACATGG CATGGACGAG   781 CTGTACAAGT AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA   841 CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT   901 GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAGCAAT AGCATCACAA   961 ATTTCACAAT AAAGCATTTT TTTTCACTGC ATTCTAGTTG TGGTTTTGTC CAAACTCATC  1021 AATGTATCTT AACGCGTCGA TCATATTCAT AACCTTATAT AACTTCGTAA TAATGTATGC  1081 TATTACGAGG TTATTAGGTC TGAGAGAGTT AACGTTCCAG CAAGCTTAGG AACCTCGAAC 1141 TCTCGAGACT //
Primer: PGKpro_F (Pr0249) Region: PGK pro,insert mid(HygR) Sequence file: RDB13921_A6B8f.seq
>D03070A05_A6B8_1_PGK-proF_A07_01.ab1     1 ACTACGCGTT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT   121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG   181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA   241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC   301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC   361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT   421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG   481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC   541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC   601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA   661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC   721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC   781 AACATCTTCT TCTGGAGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC   841 GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC   901 TTGACCAACT CTATCAGAGC TTGGTTGACG CAATTTCGAT GATGCAGCTT GGGCGCAAGG   961 GTTCGATGCG ACGCATCGTT CCGATCGGAG TCTGGGACTG TCGGGCGTAC ACAAATCGCC  1021 GCAGAGCGCG CCGTCTTGGA ACCGAATGCT GTGTAGAGTA CCTCGCGATA GTGAAATCGG  1081 ACGCTCAGCA CCTGTCGAGC AAGGATTGCC TTAGCTGCGG CCCCTCACGA CTCGCTGAGC 1141 TGTC //
Primer: PGK_N_rev (Pr0243) Region: lox P,SV40p(A),insert mid(mClover(A206K) Sequence file: RDB13921_A6B8g.seq
>D03070A05_A6B8_1_PGK-N-rev_A06_03.ab1     1 CATGGGCGCT CCCTACCGGT AGATTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA    61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT   121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT   181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT   241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA   301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA   361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCATGTGTA ATCCCGGCGG   421 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT   481 TGGACTGATG GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG   541 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCAACGTTG TGGCGGATCT   601 TGAAGTTAGC CTTGATGCCG TTCTTCTGCT TGTCGGCCGT GATATAGACG TTGTGGCTGT   661 TGAAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT   721 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT   781 AGGTACCGTC GTCCTTGAAA GAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG   841 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAAGCA GCCACGCCGT   901 AGCCGAAGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA   961 ACTTCAGGGT CAAGCTTGCC GTTTGGTGGC ATCGCCCTCG CCCTCGCCCG CGGACGCTGA  1021 ACTTTGTGGC CGTTAACGTC GGCCGTCAAG CTCGACCAGA TGGCACCACC CCGGTGAACA  1081 GCTCTTCGCC ATGCCTCACA ATGCTAGCGC GCATGCACGG GATCCGAGCT CCATTTCGCC 1141 CCTATTTAGT GAAGATCCGC A //
Primer: M13R (Pr0160) Region: lox P,mPGK ter,HygR Sequence file: RDB13921_A6B8h.seq
>D03070A05_A6B8_1_M13R_B01_04.ab1     1 CTTCGCGCAT TACCCTCACT AAAGGGAACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC    61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG   121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT   181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC   241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC   301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT   361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT   421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA   481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC   541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA   601 TATCATCGAT GCATGGGGTC GTGCGCTCCT TTCGGTCGGG CGCTGCGGGT CGTGGGGCGG   661 GCGGCTAGCC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC   721 GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG   781 CCCCGACAGT CCCGGCTCCG GATCGGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT   841 GCATCATCGA AAATTGCCCG TCAACCAAAG CTCTGATAGA GTTTGGTCAA GACCAATGGC   901 GGGAGCATAA TACCGCCCGG GGAGCCCGCG GGCGAATCCT GCAAGGCTCC GGGATGCCTC   961 CGGCTCGAAA GTAAGCGCGT CTGGCTGGCT CCATACAAGG CCAACCACGG GCCTTCCAGA  1021 AAGAAGATGT TTGGGGACCC TCGTATTGGT GAAAATCTCC CGAACAGTCG GCCCTCCGCC  1081 TCCAGTTCAA TGAACCGCGC TGTTTAATGC GGGGCCTATT TGTTCCGACT CAGGGGACAT 1141 ATTGTGTT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

GNP_filter3_RDBDEP_html_231001.pl