Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK280 (mCherry2-NeoR)

A plasmid for construction of C-terminal mCherry tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13923_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5' Linker (mCherry2 5') Sequence file: RDB13923_A6B8e.seq
>D03070A07_A6B8_1_M13(-40)_D02_11.ab1     1 NNNNNNNNCN CNNNNNNGNN TNNCNATAGG GCGAATTGGA GCTCGGATCC GGTGCAGGCG    61 CCGCTAGCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC   121 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG   181 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC   241 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG   301 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAATT   361 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC   421 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG   481 GCCCCGTAAT GCAGTGTCGT ACCATGGGCT GGGAGGCCTC CACTGAGCGG ATGTACCCCG   541 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT   601 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT   661 ACAACGTCGA CATCAAGTTG GACATCCTTT CCCACAACGA GGACTACACN NNNNNNNNAA   721 CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGTAA   781 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA   841 AACCTCCCAC ACCTCCCCNN GAACCTGAAA CATAAAATGA ATGNAATTGT TGNTGNTNAC   901 TTGNTTATTG NANCTTATAA TGNNTACNAN TAAAGCAATA GCATNNCAAA NTTNNNAAAT   961 NANNCATTTT TTNCACTGNN NNCNANTNNT GGNTNNNNNN ANCTCNTNNN GNNNNNNNNN 1021 N //
Primer: M13R (Pr0160) Region: insert 3'(TK pA,NeoR_KanR 3') Sequence file: RDB13923_A6B8f.seq
>D03070A07_A6B8_1_M13R_D01_10.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNAAGCTGGG TACCGGGCCC CCCCTCGAGG    61 GNNCCAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG   121 TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA   181 ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC   241 CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG   301 GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG   361 GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG   421 GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG   481 ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC   541 CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG   601 CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC   661 CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG   721 TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC   781 ATCANCCATG ATGGATACTT TCTCGGCAGG AGCAANGTGA GATGACAGGA GATCCTGCCC   841 NNGCACTTCG CCNNATANCA GCNAGTCCCT TCCCGCTTCN GTGANAACGT CNAGNNNNAG   901 CTGCNNAAGG AANNGCCCGT CGNGGNCNNG CCACGATNNN NCNGCGCTGC CTNGTCTNGN   961 NNTTTNATTC NNGGGNNCCN NNNNNGNNNN GNTCTTNNNN AAAAAANAAN NNGGNNNNNC 1021 CNGNNNCNNA NNNCCNGNNN NNNNNNN //
Primer: SV40polyA-R (Pr0109) Region: insert mid(mCherry2 3') Sequence file: RDB13923_A6B8g.seq
>D03070A07_A6B8_1_SV40polyA-R_G04_19.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNCTCTNN NNNGTGGTAT GGCTGATTAT GATCAGTTAT    61 CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG   121 TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA TGTCCAACTT GATGTCGACG   181 TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG   241 TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG   301 TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA TGGTACGACA CTGCATTACG   361 GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC   421 TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC   481 TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT CGGGGATGTC GGCGGGGTGC   541 TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA GGATGTCCCA GGCGAAGGGC   601 AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC   661 TCGCCCTCGC CCTCGATCTC GAACTCNNGG CCGTTCACGG AGCCCTCCAT GTGCACCTTG   721 AAGCGCATGA ACTCCTTGNN NGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATGCT   781 AGCGGCGCCT GCACCGGATC CGAGCTCCAA TTCGCCCTAT NNNTGAGTCN NNTTANNCGC   841 GCTCACTGNN NNNCNTTTNA CANNGTCNTG NCTGGGNAAA ANNCNTGNNN TNNNNCNANN   901 TTNANTCGNN NTGCANNNNN TNCCCCCTTT NGCCAGCTGG NNNNATNNNC NAANANGNCC   961 NCANNGATNN NCCNTNCCNN NNNNNNGCNC NNCCNNNAAN GNNNNANNNN AAANNNNNNN 1021 NNN //
Primer: SV40pro_F_V2 (Pr0154) Region: insert mid(NeoR_KanR 5') Sequence file: RDB13923_A6B8h.seq
>D03070A07_A6B8_1_SV40pro_F_V2_B10_04.ab1     1 NNNNNNNNNN NNNNGNNNNN NGCTTTTGNA AGNNCGNTCA AGAGANAGGN NGAGGNNCGT    61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC   121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC   181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG   241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG   301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG   361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG   421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC   481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG   541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC   601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG   661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC   721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC   781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CNGATTCGCA GCGCATCGCC TTCTATCGCC   841 TTCTTGACGA NTTCTTCTGN NCGGGNCTCT GGGGGTTCGA AATGACCGAC NNAGCGACGC   901 CCNACCNNNC NTCACGAGAT TNNGATTCCN CCGCCNCCTN NNATGAANNN NTNGGCTTNN 961 NNANNNNNTT TCCGGGNNGC CNNNNNNNTN NTNCNNNCNN NNNNGGNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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