Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK281 (mCherry2-Hygro)

A plasmid for construction of C-terminal mCherry tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13924_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5'(Linker,mCherry2 5') Sequence file: RDB13924_A6B8e.seq
>D03070A08_A6B8_1_M13(-40)_E02_14.ab1     1 NNNNNNNNNN NNNCNNNNNN GNNTNNCNAT AGGGCGAATT GGANCTCGGA TCCGGTGCAG    61 GCGCCGCTAG CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA   121 TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG   181 AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG   241 GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT   301 ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA   361 ATTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT   421 CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG   481 ACGGCCCCGT AATGCAGTGT CGTACCATGG GCTGGGAGGC CTCCACTGAG CGGATGTACC   541 CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC   601 ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG   661 CCTACAACGT CGACATCAAG TTGGACATCC TTTCCCACAA CGAGGNCTAC ACCATCGTGG   721 AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAN CTGTACAAGT   781 AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTNA   841 AAAANCTCCC ACACCNCCCC CNGANNCTGA AACATAAAAN GAATGCNATT GTTGTTGNTA   901 ACTTGTTNAT TGCAGCTTNN AATGNTTACN AATAAANCAA TAGCNTCNCN ANNTTCACAA   961 ATAAAGCATT TTTTTCNCTG NNNTCTANNN GNGGNTNGNN CNAANNCATN NNNGTATNNN 1021 NNNNNNN //
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter,HygR) Sequence file: RDB13924_A6B8f.seq
>D03070A08_A6B8_1_M13R_E01_13.ab1     1 NNNNNNNNNN NNNNNNNNNN NANGNNNNNN AAGCTGGGTA NCGGATCCTA GTGAACCTCT    61 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG   121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA   181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC   241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT   301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT   361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA   421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT   481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA   541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT   601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG   661 CGGCTAGCCT ATTCCTTTGC CCTCGGACGA GTGCTGGGGC GTCGGTTTCC ACTATCGGCG   721 AGTACTTCTA CACAGCCATC GGTCCAGACN GCCGCGCTTC TGCGGGCGAT TTGTGTACGC   781 CCGACAGTCC CGGCTCCNGA TCGGANGATT GCGTCGCATC GACCCTGCGC CCAAGCTGNN   841 NTCATCGAAN NTGCCGTCNA CCAANNCTCT GANAGAGTTG GTCNNGANCA ATGCNGNANC   901 ATATACGCCC NGNANNCNCN GNNATCCTGC NANCNNNNGA TGNCNNNCNC TCNNAANNNN   961 NNCGTCTGNN GCNNCNNNNN ANNNCNNANC NNNNGNNCNN NCNNNNNNNA NNNNGNNNNN 1021 NNNNN //
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mCherry2 3') Sequence file: RDB13924_A6B8g.seq
>D03070A08_A6B8_1_PGK-N-rev_C06_09.ab1     1 NNNNNNNNNN NNNNNNNNNC NGGNANANTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG    61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG   121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA   181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA   241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG   301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG   421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG   481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG   541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC   601 TCGCCCTTCA GGGCGCCGTC CTCGGGNNNN NNCCGCTCAG TGGAGGCCTC CCAGCCCATG   661 GTACGACACT GCATTACGGG GCCGTCGGAN GGGAAGTTGG TGCCNCGCAN CTTCACCTTG   721 TAGATGAACT CNNCGTCCTG CANGGANGAN NNNNTGGGTC ACGGTCACCN NNNCNCCGTC   781 CTCNAAGTTC NTCNCGCGCT CCCNNNNNNN NNCCCTCNGG GGAAGGACAG CTTCANNNNN 841 NNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(HygR) Sequence file: RDB13924_A6B8h.seq
>D03070A08_A6B8_1_PGK-proF_C07_07.ab1     1 NNNNNNNNNN NNNNNNNNNN NTCTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT   121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG   181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA   241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC   301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC   361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT   421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG   481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC   541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC   601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA   661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC   721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC   781 AACATCTTCT TCTGGANGCC GTGGTTGGCT TGTATGGAGC AGCAGANGCG CTACTTCGAG   841 CGGANGCATC CGGANCTTGC NNGATCGCCG CGGCNNCNGG CGTATATGCT CCGCATTGGT   901 CTTGACNNAC TCTATCANAN CTNNNNTGAN NGCNATTTCN ATGANGCAGC TNNNNNCNNG   961 NTCGATGCNA CNNNNNCGTC CNATNNNGNA NNCNNGNNNN GNCNGGNNGT ACNNNANNNN 1021 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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