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pMXs-mEed

Retroviral expression vector of mouse Eed, SNP285A/T

Catalog number RDB14059
Resource name pMXs-mEed
Alternative name clone 67-9
Clone info. Retroviral expression vector of mouse Eed, SNP285A/T
Comment Expression was confirmed by the depositor.
Vector backbone pMXs Retroviral Vector (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name mouse Eed cDNA
Depositor|Developer Okazaki, Yasushi | Iseki, Hiroyoshi |
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External Database
mouse Eed

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
          Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
          付加的提供条件 pMXsベクターについて:利用者は、研究成果の公表にあたって pMXsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄先生への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14059 pMXs-mEed DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14059_A6Dcp1_v2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F2
          Sequence file: RDB14059_A6Dca.seq check
          >D03255E5_A6Dc_2_MMLV-pol_F2_E12_15.ab1
              1 TTGGGGGGGG GTGGACATCC TCTAGACTGC CGGATCTAGC TAGTTAATTA AGGATCCACC
             61 ATGTCCGAGA GGGAAGTGTC GACTGCGCCG GCGGGAACAG ACATGCCCGC GGCCAAGAAG
            121 CAGAAGTTGA GCAGCGACGA GAACAGCAAC CCGGACCTCT CGGGAGACGA AAATGACGAT
            181 GCTGTCAGTA TTGAGAGTGG CACAAACACA GAACGCCCGG ACACGCCCAC AAATACGCCA
            241 AATGCACCAG GAAGGAAAAG CTGGGGAAAG GGAAAATGGA AGTCAAAGAA ATGCAAATAT
            301 TCTTTCAAAT GTGTGAACAG CCTCAAGGAA GATCATAACC AGCCATTGTT TGGAGTTCAG
            361 TTTAACTGGC ACAGTAAAGA AGGAGACCCT CTGGTGTTTG CAACTGTGGG AAGCAACAGA
            421 GTAACCTTAT ACGAATGCCA TTCACAGGGG GAGATACGGT TATTGCAGTC CTATGTAGAT
            481 GCTGATGCAG ATGAAAACTT TTACACTTGT GCATGGACCT ATGATAGCAA CACCAGCCAC
            541 CCTCTATTAG CAGTTGCTGG ATCTAGAGGC ATTATAAGAA TAATTAATCC TATAACAATG
            601 CAGTGTATAA AGCACTATGT TGGCCATGGA AATGCTATCA ATGAGCTGAA ATTCCACCCA
            661 CGAGACCCAA ACCTTCTCCT GTCAGTAAGT AAAGATCATG CTTTACGGTT ATGGAATATC
            721 CAAACAGACA CTCTTGTGGC AATATTCGGA GGTGTGGAAG GGCACAGAGA TGAAGTTCTG
            781 AGTGCTGATT ATGATCTTTT GGGTGAAAAA ATAATGTCCT GTGGTATGGA TCACTCTCTT
            841 AAACTGTGGA GAATCAACTC AAAGAGGATG ATGAATGCAA TTAAGGAGTC TTATGATTAT
            901 AACCCAAACA AAACTAACAG GCCATTTATT TCCCAGAAAA TCCACTTTCC TGACTTTTCT
            961 ACCAGAGACA TACATAGGAA TTATGTTGAT TGTGTGCGAT GGTTAGGCGA TTTGATACTT
           1021 TCCAAGTCTT GTGAAATGCC ATTGTATGCT GGAAAACCTG GCAAATGGGA GGATGAATAT
           1081 AGAATAAACT CAACCTAGGT GAGGTCTATG GTGACCTATC TGGGCGAATT GGAATTTACA
          1141 GCCCAGTGTG ACATTTTTGG TTTACATTGA GGGCT
          //
          Primer: pMXs-3LTRup_R2
          Sequence file: RDB14059_A6Dcb.seq check
          >D03255E5_A6Dc_2_pMXs-3LTRup_R2_G12_21.ab1
              1 GCGGGTAAAA TCTTTTATTT TATCGTCGAG TTATCGAAGT CGATCCCATC GCCAAATGCT
             61 GGCATCATCG CAGACAGCTA TGAGGATGCT GCTATCCCTA CTGAAACTGG TTTGTCGAAT
            121 AGCCGCGCCA CATTTATGAT GGGTCAGTGT TGTGCATTTG GCTTTATGAG GATCTTCTAC
            181 TTCTAAATCC CAAACATACA GTTTGCCAAC CTGATTGCCC AATGCAAGCA TCTTTTGCCA
            241 GAAATCCATA GAAAACCTCA TGTACCAAAT GTCACACTGG CTGTAATCAA ATCGCCCAAG
            301 AATAGTCACA TTAGACTCAC TAGGTTTAAT TTTATCTATA TCATCCTCCA TTTTGCCAGG
            361 TTTCCAGCAT ACAATGGCAT TTTCACAAGA CTTGGAAAGT ATCAAATCGC CTAACCATCG
            421 CACACAATCA ACATAATTCC TATGTATGTC TCTGGTAGAA AAGTCAGGAA AGTGGATTTT
            481 CTGGGAAATA AATGGCCTGT TAGTTTTGTT TGGGTTATAA TCATAAGACT CCTTAATTGC
            541 ATTCATCATC CTCTTTGAGT TGATTCTCCA CAGTTTAAGA GAGTGATCCA TACCACAGGA
            601 CATTATTTTT TCACCCAAAA GATCATAATC AGCACTCAGA ACTTCATCTC TGTGCCCTTC
            661 CACACCTCCG AATATTGCCA CAAGAGTGTC TGTTTGGATA TTCCATAACC GTAAAGCATG
            721 ATCTTTACTT ACTGACAGGA GAAGGTTTGG GTCTCGTGGG TGGAATTTCA GCTCATTGAT
            781 AGCATTTCCA TGGCCAACAT AGTGCTTTAT ACACTGCATT GTTATAGGAT TAATTATTCT
            841 TATAATGCCT CTAGATCCAG CAACTGCTAA TAGAGGGTGG CTGGTGTTGC TATCATAGGT
            901 CCATGCACAA GTGTAAAAGT TTTCATCTGC ATCAGCATCT ACATAGGACT GCAATAACCC
            961 GTATCTCCCC CTGTGAATGG CATTCGTATA AGGTTACTCT GTTGCTTTCC CACAGTTGCA
           1021 ACACCAGAGG TCTCTTCTTT ACTGTGCCAG TAAACTGACT CAAACATGCT GGGTTATGAT
           1081 CTCGTGAGCT GTTCACACAT TGAAGAATAT TGCAATTTCT GACCTCAATT TCCCTTTTTC
          1141 CCAAGCCAT
          //
          Primer: Amp_R
          Sequence file: RDB14059_A6Dcc.seq check
          >D03255E5-2_A6Dc_Amp_R_E01_05_ABI08.ab1
              1 CTCCCTTTTT TAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
             61 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
            121 GCCACCTGCA TAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA
            181 TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAAA AGTTCAGATC AAGGTCAGGA
            241 ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC
            301 GGCTCAGGGC CAAGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
            361 AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCAGCCCT
            421 CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC
            481 TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT
            541 CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATTGAC
            601 TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG
            661 TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT
            721 TCATTTGGGG GCTCGTCCGG GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC
            781 GGGAGGTAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG
            841 ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG
            901 GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG GGAGACGTCC CAGGGACTTC
            961 GGGGGCCGTT TTTGTGGCCC GACCTGAGTC CAAAAAATCC CGATCGTTTT TGGACTCTTT
           1021 GGTGCACCCC CCCTAATAAG GAGGGATATG TGGATTCTGG TAGGGAGACG AGAACCTAAA
           1081 ACAGTTCCGC TTCCGTTCTG AAATTTTGCT TCCGGATTGG ACGGAAGCCG CGCCGCGCGG
          1141 TCTTTATTCT GGCCTGGCCA G
          //
          Primer: pMFG_1
          Sequence file: RDB14059_A6Dcd.seq check
          >D03255E5-2_A6Dc_pMFG_1_F01_06_ABI08.ab1
              1 GGATGTGGGA AAGGGGCTGT TAGTACTCTT GTCATGTCAG GGTCAGGGAA GTTTACAAGG
             61 GGCGGGGGTG CCCCATATAA GATCTCATAT GGCCATATGG GGGCGCCTAG AGAAGGAGTG
            121 AGGGCTGGAT AAAGGGAGGA TCGAGGCGGG GTCGAACGAG GAGGTTCAAG GGGGAGAGAC
            181 GGGGCGGATG GAGGAAGAGG AGGCGGAGGC TTAGGGTGTA CAAAGGGCTT GACCCAGGGA
            241 GGGGGGTCAA AAGCCAAGGC TTCCCAGGTC ACGATGTAGG GGACCTGGTC TGGGTGTCCA
            301 TGCGGGCCAG GTGAAAAGAC CTTGATCTTA ACCTGGGTGA TGAGGTCTCG GTTAAAGGTG
            361 CCGTCTCGCG GCCATCCGAC GTTAAAGGTT GGCCATTCTG CAGAGCAGAA GGTAACCCAA
            421 CGTCTCTTCT TGACATCTAC CGACTGGTTG TGAGCGATCC GCTCGACATC TTTCCAGTGA
            481 CCTAAGGTCA AACTTAAGGG AGTGGTAACA GTCTGGCCCT AATTTTCAGA CAAATACAGA
            541 AACACAGTCA GACAGAGACA ACACAGAACG ATGCTGCAGC AGACAAGACG CGCGGCGCGG
            601 CTTCGGTCCC AAACCGAAAG CAAAAATTCA GACGGAGGCG GGAACTGTTT TAGGTTCTCG
            661 TCTCCTACCA GAACCACATA TCCCTCCTAT TAGGGGGGTG CACCAAAGAG TCCAAAACGA
            721 TCGGGATTTT TGGACTCAGG TCGGGCCACA AAAACGGCCC CCGAAGTCCC TGGGACGTCT
            781 CCCAGGGTTG CGGCCGGGTG TTCCGAACTC GTCAGTTCCA CCACGGGTCC GCCAGATACA
            841 GAGGCTAGTT AGGCTAACTA GTACCGACGC AGGCGCATAA AATCAGTCAT AGACACTAGA
            901 CAAATCGGAC AGACACAGAT AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG
            961 TTCCCTGGGC AGGGGTCTCC CGGATCCCGG ACGAGCCCCC AAATGAAAGA CCCCCCGCTG
           1021 GACGGTAGTT CATCACTCAG AGGAGACCCT TCCCCAAGGA ACAGCGGAGA CCCACAAGGT
           1081 TCGGATGCAA CTGGCAGAGG TTTTATTTGG ATACACGGGT AACCCGGGGC GACCTCTCAG
          1141 TCCAAATCT
          //
          Primer: SV40pA3'Element Reverse
          Sequence file: RDB14059_A6Dce.seq check
          >D03255E5-2_A6Dc_SV40pA3'ElementReverse_G01_07_ABI08.ab1
              1 GGTTCAAGGG GGGGGCGGAG CCTATGGAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
             61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
            121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
            181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG
            241 CGCGTTGGCC GATTCATTAA TGCAACTATG GCCATTTAAT GTAAATACTT AAGAAAAAAA
            301 ACCAAATTAA TTTTGATACA TGCTGCATGT GAAAGACCCC CGCTGACGGG TAGTCAATCA
            361 CTCAGAGGAG ACCCTCCCAA GGAACAGCGA GACCACAAGT CGGATGCAAC TGCAAGAGGG
            421 TTTATTGGAT ACACGGGTAC CCGGGCGACT CAGTCAATCG GAGGACTGGC GCCCCGAGTG
            481 AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG GGAGCAGAAG CGCGCGAACA GAAGCGAGAA
            541 GCGAACTGAT TGGTTAGTTC AAATAAGGCA CAGGGTCATT TCAGGTCCTT GGGGCACCCT
            601 GGAAACATCT GATGGTTCTC TAGAAACTGC TGAGGGCTGG ACCGCATCTG GGGACCATCT
            661 GTTCTTGGCC CTGAGCCGGG GCAGGAACTG CTTACCACAG ATATCCTGTT TGGCCCATAT
            721 TCAGCTGTTC CATCTGTTCT TGGCCCTGAG CCGGGGCAGG AACTGCTTAC CACAGATATC
            781 CTGTTTGGCC CATATTCAGC TGTTCCATCT GTTCCTGACC TTGATCTGAA CTTCTCTATT
            841 CTCAGTTATG TATTTTTCCA TGCCTTGCAA AATGGCGTTA CTTAAGCTAG CTTGCCAAAC
            901 CTACAGGTGG GGGTCTTTCA TTCCCCCCCT TTTTTCTGGA GACTAAATAA AATCTTTTTA
            961 TTTTTATCGT CGAGTTTATC GAAGTCGATC CCATCGCCCA AATGCTGGGC ATCATCGCAG
           1021 ACAGCTATGA GGGATGCTGC TTATCCCTAC TGAAACTGGG TTTGTTCGAA TAGGCCGCGC
           1081 ACATTTTATG ATGGGTTCAG TTGTGTGCCA TTTTGGCCTT TATGGAAGGG AATCATTCCT
          1141 TACCGTTCCG TA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl