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pCAGIPuro-HAmSuz12

Expression vector of HA tagged mouse Suz12.

Catalog number RDB14116
Resource name pCAGIPuro-HAmSuz12
Alternative name clone 6
Clone info. Expression vector of HA tagged mouse Suz12.
Comment Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse Suz12 cDNA
Depositor|Developer Okazaki, Yasushi | Iseki, Hiroyoshi |
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External Database
mouse Suz12

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
          Additional terms and conditions for distribution CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
          付加的提供条件 利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB14116 pCAGIPuro-HAmSuz12 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14116_A6Dcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB14116_A6Dca.seq check
          >D03255K8_A6Dc_pAxCA_F1_H02_23_ABI24.ab1
              1 CTTACGCAAT CTGCTACATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
             61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGACCACC ATGTACCCTT
            121 ACGACGTGCC TGACTACGCC GGCGGCGGAA TGGCGCCTCA GAAGCACGGC GGTGGGGGAG
            181 GGGGCGGCTC GGGGCCCAGC GCGGGGTCCG GGGGAGGCGG CTTCGGGGGT TCGGCGGCGG
            241 CGGTGGCGGC GGCGGCTTCG GGCGGCAAAT CCGGCGGCGG GGGCTGTGGA GGCGGCGGCA
            301 GTTACTCGGC CTCCTCCTCC TCGGCGGCGG CCGCGGCGGC GGCGGCGGGG GCCGCGGTGT
            361 TGCCGGTGAA GAAGCCGAAA ATGGAGCACG TCCAGGCTGA CCACGAGCTT TTCCTCCAGG
            421 CCTTTGAGAA ACCAACACAG ATATATAGAT TTCTTCGAAC TCGAAATCTT ATCGCACCAA
            481 TATTTTTGCA CAGAACTCTT ACTTACATGT CTCATCGAAA TTCCAGAACA AGCATCAAAA
            541 GGAAAACATT TAAAGTTGAT GATATGTTAT CAAAAGTAGA GAAAATGAAA GGAGAGCAAG
            601 AATCTCATAG CTTGTCTGCA CATTTGCAAC TTACATTTAC CGGTTTCTTC CACAAAAATG
            661 ATAAGCCATC ACAAAACTCA GAAAATGAAC AAAATTCTGT TACCCTGGAA GTCCTGCTTG
            721 TGAAAGTTTG CCACAAAAAA AGGAAGGATG TAAGTTGTCC AATAAGACAA GTCCCTACTG
            781 GTAAAAAGCA GGTGCCTTTG AATCCTGACC TCAATCAAAC AAAACCAGGA AATTTTCCAT
            841 CCCTGGCAGT TTCCAGTAAT GAATTTGAAC CTAGTAACAG CCATATGGTG AAGTCCTACT
            901 CGTTGCTGTT TAGAGTAACT CGTCCAGGGA GAAGAGAATT TAAATGGAAT GATTAATGGA
            961 GAAACCCAAT GAAATATTGA TGTCAGTGAG ACCTTTCCAA GCCCAGAGAA AACGAAATTC
           1021 GCGAGATGGA GGAAAAGACA TTTTGTGGCA CAATTGACCA GGTTTTTGAC TACACAGCGA
          1081 TTCACAGCCT TTTAGAATTG GGGCCA
          //
          Primer: IRES-R
          Sequence file: RDB14116_A6Dcb.seq check
          >D03255K8_A6Dc_IRES-R_F09_18_ABI24.ab1
              1 GGCAAGCGGG CTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGGGGG GGGCGGAATT
             61 CGATATCAAG CTTATCGAGC GGCCGCTCGA TACCGTCGAT CTCAGTGGTA TTTGTGAGCC
            121 AGTTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTGAATTCCT
            181 CGACTCAGAG TTTTTGTTTC TTGCTCTGTT TTGGAACCCC TGAGACACCG TCAGTTTCCA
            241 AAGCTTTCTC TTTTGAGTTA GTTTCACTGA ATCCATTTGC TGTTCCATTT TGTTCCTCAG
            301 CTATTTCTTC ATTTGAAGGG GTCGCAGATT CTCCTTTTTC TAGTTTTTGC TGCATTTCTC
            361 GGAGCTTGGT AACAGCTTTA TCTATTGACA TTATGCTAAT AAGATTAAAG TCATGCATGC
            421 TGACTAGATG AAGCATGAAG TTTCGACATA AATTCTTCTT AATTATTTTC TGTCCATAAT
            481 TTTCTACAAA CAGCATACAG GCATGATTCA TTTGATTGTC AGCAATAAAT CCATGCTTCA
            541 TGACATGGAG GTTCCACAGC TTCATCACTT CTTTCTCTCC TTCATTCACA TCAGAAAAAT
            601 TCTTCAATTT GAGTAATGGT TTTTTCTCTC AGCCATTCTG GATCTTTCTC ATCTTCACTA
            661 TCTACTTCCA TTTCTTGTGG CCGAAGAGGT AAGCAGTATC ACTGTGGAAA TAGAGACGAT
            721 TGTGGCCACT GCTGTATGTT CTCTGCTGCT CCACTTCTCC ATCTTTCAGA TTCAAGAAAC
            781 TCCGACATGC TTGCTTTTGT TCTTTTTGGC CCTGCAAACA AGAATATGTG TGATAGGTGT
            841 TCTCTTTACC GGGTCCAATT CGACTAAAGC AAATCCCAGG TTTGGCGATG TATTATCCTG
            901 AGATTCCTGC CATAGGAAGC ATCATAACAC CTCATTGATG GAAACATCTA TCTAGCACTT
          961 TTGGATGAAT ATACGTAATT GAATGACCTG CTGTGCAAGC CTTTTCAGAA TGCTG
          //
          Primer: IRES-F
          Sequence file: RDB14116_A6Dcc.seq check
          >D03255K8_A6Dc_IRES-F_A03_03_ABI24.ab1
              1 GTAAAGCTTT ATGTGTTTAG TCGAGGTTAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
             61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
            121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
            181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
            241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
            301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
            361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
            421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
            481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
            541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
            601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
            661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
            721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT
            781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
            841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
            901 CGCATTGTCT GAGTAGTGTC ATTCTATTCT GGGGGGGTGG GGTGGGGCAG GACAGCAAAG
            961 GGGGAGGATT TGGGAAGACA ATAGCAGGCA TGCTGGGGGA TGCGGTGGCT CTATGGCTTT
           1021 CTGAGCGGAA AGAACCCTGC AGCCCAAGCC TGCGTAATCA TGGTTCAATA GCTGATACCT
          1081 GTGTGTGAAA TTGTTATCCG CCTCACCAAT TCCACACACA GAC
          //
          Primer: BGHpA_F
          Sequence file: RDB14116_A6Dcd.seq check
          >D03255K8_A6Dc_BGHpA_F_A06_03_ABI24.ab1
              1 TCGTAAGGTT ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
             61 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA
            121 ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
            181 CCGCTCACAA TTCCACACAA CATACGAGCC GAAAGCATAA AGTGTAAAGC CTGGGGTGCC
            241 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
            301 AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
            361 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
            421 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
            481 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC
            541 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
            601 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
            661 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
            721 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
            781 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
            841 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
            901 CATAGGCTCC GCCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
            961 AAACCCGACA GGACTATAAA GATACCCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
           1021 CTTCTGTTCG ACCCTGCGCT TTACCGGATA CTGTCGCGTT TTCTCCGTTC CGGTAGCCGT
          1081 TGCGCTTTCT CATAGCTCCA CCGCTGATAG GATAATTCTC A
          //
          Primer: Amp_R
          Sequence file: RDB14116_A6Dce.seq check
          >D03255K8_A6Dc_Amp_R_A10_01_ABI24.ab1
              1 CTCGTTTTCA TGACGCTTTG GAAACAAAGA CTGTATTTCC TGGAAATTAA TGTTTATTCA
             61 ATAAACTGTG TATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG
            121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC
            181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA
            241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG
            301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA
            361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG
            421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT
            481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC
            541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC
            601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
            661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG
            721 ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT
            781 AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG
            841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
            901 CGCCCAATAG GACTTTCCAT TTGACGTCAA TGGGGTGGGA GTATTACGTA AACTGCCCAC
            961 TTGGCAGTTA CATCCAGTGT ATCATAATGC TAGTTACGCC CCCTATGACG GTCATGAACG
          1021 GTAATGACCG GCCTGGCATA TGCCAGTACA TTGACTTATT GGGGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl