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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGIPuro-HAmJarid2WT

Expression vector of HA tagged mouse Jarid2, wild type.

Catalog number RDB14117
Resource name pCAGIPuro-HAmJarid2WT
Alternative name clone 14
Clone info. Expression vector of HA tagged mouse Jarid2, wild type.
Comment Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse Jarid2 cDNA
Depositor|Developer Okazaki, Yasushi | Iseki, Hiroyoshi |
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External Database
mouse Jarid2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
          Additional terms and conditions for distribution CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
          付加的提供条件 利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB14117 pCAGIPuro-HAmJarid2WT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14117_A6Dcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB14117_A6Dca.seq check
          >D03255K9_A6Dc_pAxCA_F1_B03_06_ABI24.ab1
              1 GTGAGGGGGT CCTGCTACAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
             61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CCTCGACCAC CATGTACCCT
            121 TACGACGTGC CTGACTACGC CGGAGGAGGC ATGAGCAAGG AAAGACCCAA GAGGAATATC
            181 ATTCAGAAGA AATACGATGA CAGCGATGGG ATCCCGTGGT CAGAAGAGAG AGTTGTACGA
            241 AAAGTCCTGT ATTTGTCCCT AAAGGAATTC AAGAATGCAC AGAAAAGGCA GCATGGGGAA
            301 GGCCTTGCCG GGAGCCTGAA GGCGGTAAAT GGGCTTCTTG GTAATGCCCA GGCTAAGGCA
            361 CTAGGACCAG CCTCAGAGCA GTCAGAGAAC GAGAAGGATG ATGCCTCCCA AGTGTCCTCT
            421 ACTAGCAACG ATGTTAGTTC TTCAGATTTT GAAGAAGGGC CGTCGAGGAA AAGGCCCAGG
            481 CTGCAAGCAC AAAGGAAGTT TGCTCAATCT CAGCCGAATA GTCCCAGCAC AACTCCAGTG
            541 AAGATAGTGG AGCCTTTGCT ACCCCCGCCA GCTACTCAAA TTTCTGACCT CTCTAAAAGG
            601 AAGCCTAAGA CAGAAGACTT TCTTACCTTT CTCTGCCTTC GAGGTTCTCC TGCGCTGCCC
            661 AACAGTATGG TATATTTTGG AAGCTCTCAG GATGAGGAGG ATGTCGAAGA GGAAGATGAT
            721 GAGACGGAAG ATGTCAAAGC AACCACCAAC AATGCTTCAT CTTCGTGCCA GTCAACCCCC
            781 AGGAAAGGAA AAACCCATAA GCATGTACAC AACGGGCACG TTTTCAATGG CTCCAGTATG
            841 TCAGCACGGG AGAAAGAGCC TGCTCACAAA CACAGAAGCA AAGAGGCCAC TCCGGGGAAA
            901 GGAGAAGCAC AGCGAGCCCA GGCAGACAGC CGGGAGAGAA CAGGCTTCAG GGGCTCAGCC
            961 CACGGCCGCC TCGGCTGCGG CTTTCCTCTG CCAAGGGACT TGCTGCCACA CCAACCTCCC
           1021 CCTTTCGCAT CGGTCTGCTC AAGACTTTAC GAACCAGTGT CTAGTAAATT GTGTCACTCG
          1081 TATGTCATCT CTTGGTGCAA GTACCAAAAC AAG
          //
          Primer: IRES-R
          Sequence file: RDB14117_A6Dcb.seq check
          >D03255K9_A6Dc_IRES-R_C03_09_ABI24.ab1
              1 GGACATGCAG CGTGCGACAG TGCGTGAGGC GGGGGGGAGG GAGAGGGGGG GGGGGCGGAA
             61 TTCGATATCA AGCTTATCGA GCGGCCGCTC GATACCGTCG ATCTCAGTGG TATTTGTGAG
            121 CCAGTTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
            181 CTCGACTCAT GAGGATGGGA GCCGAGATGG CGGCACATCC ACGGTCGCTC TCTTGCGGGG
            241 ACCTCTTTTT GGTGTGGGTT TGTTGAGACA GTTCTCGATG CCACCGTGTT TGCCAGACAC
            301 TTTGCCACAT ATCTGGTTGA CCAAACTGAT GATCTGCTCC TCATCGTAGC GGTACATCAG
            361 CTTCAGCCCA CGGCAAGACT TCTGCTTCTC CACATGGCGC AGTGCACACT CCAGGCAGAA
            421 CACGACATTC TCATTCTCCT GTACCACCAT GGACAGGTAG CACAAGTGCT GGCAGATCTG
            481 GCACCTCCTT TCTGAAGTTT CCAGCTGTAG CCACTTTCGA GGCTTTTTCT TCCCATCCGC
            541 CACTGTGCTG TTGCCGTCGT GGCTGCCATA GCGTGCAGAA GAGTGAAGGC CAGCCTCGAA
            601 CAACAGCCTG CGCTGCCGCA GCTCTGTATC CCTGAGCTCA TCCAGAAGGG CTGAGATGGT
            661 ACTGAGGGTG GGACCATTTT CCTTTTTTGC TTCTGCTTGT GCAATCTGGT AGAGCAACTT
            721 CTCCATGGAG AATGGCTTAG CTATATGGCG GCGCTTCATC TCCTTGGCAG TCTCGAAGCC
            781 CATGCTTGTC CACTGGGTGG TAGCAAAGTG CACTGTCTCT GAGACGTTGT AGCCACAACA
            841 TACTTTGGAC ACAAAGGATC CCGGGAAGCA GACGACAAAC TGCCCACTCT GCTGTACAGT
            901 CCTGTGGACC TTGATCCCCT TTCTTGCACA GCACCTCTGG GGAGATCATT ACATTGCTCT
            961 CCAGCATCTG CAGCCCTGGG GTACCATTGC CCTGCAGCAG AGTGTGACTA CGTCCTCCAG
           1021 CCTGGTCTCT CTCAGCAAGA TGCATTACGA ATTGCAGTCA GCACCATGTG TAAGTAGTCA
          1081 TATATGGCAA GTGAATTTTG GATCCTCCGG GAG
          //
          Primer: IRES-F
          Sequence file: RDB14117_A6Dcc.seq check
          >D03255K9_A6Dc_IRES-F_D03_12_ABI24.ab1
              1 GGCAGTCGTT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
             61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGAC CGAGTACAAG
            121 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC
            181 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG
            241 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG
            301 GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG
            361 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG
            421 CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG
            481 GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC
            541 CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC
            601 CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC
            661 GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACGCCC GCCCCACGAC
            721 CCGCAGCGCC CGACCGAAAG GAGCGCACGA CCCCATGCAT CGATGATCTA GAGCTCGCTG
            781 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC
            841 TTCCTTGACC CTGGAAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG
            901 CATCGCATTG TCTGAGTAGT GTCATTCTAT TCTGGGGGGG TGGGGTGGGG CAGACAGCAA
            961 GGGGGAGGAT TGGGAAGACA TAGCAGGCAT GCTGGGGGAT GCGGTGGGCT CTATGGCTTT
           1021 CTGAGCCGAA AGAACTGCAG CCCAGCCTGG CCGTATTCAT GGTCATAGCC TGATTCCTGT
          1081 GGTGAATGGT AATCCGGCCT CAACAATTTC ACCA
          //
          Primer: BGHpA_F
          Sequence file: RDB14117_A6Dcd.seq check
          >D03255K9_A6Dc_BGHpA_F_E10_13_ABI24.ab1
              1 TTGTAATGTT ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
             61 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA
            121 ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
            181 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
            241 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
            301 AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
            361 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
            421 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
            481 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC
            541 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
            601 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
            661 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
            721 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
            781 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCACGGG ATAACGCAGA AAGAACATGT
            841 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
            901 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
            961 ACCCGACAGG ACTATAAGAT ACCAAGGCGT TCCCCCCTGG AGCTCCCTCG TGCGCTCCTC
           1021 TGATCGACCC TGCGCTTACT GCATACTGTC GCATTCTCTT CGGTAAGCGT GCGCATTCTC
          1081 ATAGGCTCAC AGC
          //
          Primer: Amp_R
          Sequence file: RDB14117_A6Dce.seq check
          >D03255K9_A6Dc_Amp_R_F10_16_ABI24.ab1
              1 CTCGTTTTCA TGACCGCTTT GGAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC
             61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
            121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
            181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
            241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
            301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
            361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
            421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
            481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
            541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
            601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
            661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
            721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
            781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
            841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT TCCCATAGTA
            901 ACGCCAATAG GGACTTTCCA TTGACGTCAT GGGGTGGAGT ATTTACGGTA ACTGCCCACT
            961 GCAGTACATC AGTGTATCAT ATGCAGTACG CCCCCTATTG ACGTCCATGA CCGTAATTGA
          1021 CCGCGTGGCA TTATGGCCAG TACATGACGT ATGACGTTCC TACTGCAGTA CATCTTACG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl