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pDll1-Ub-Fluc

Reporter vector for time-lapse imaging of Dll1 transcription.

Catalog number RDB14127
Resource name pDll1-Ub-Fluc
Clone info. Reporter vector for time-lapse imaging of Dll1 transcription. This construct contains Dll1 promoter and destabilized luciferase cDNA.
Comment Expression was confirmed by the depositor with bioluminescence imaging. See Fig.1 of Shimojo, H. et al., Genes Dev. 30 (1): 102-116, 2016.
Vector backbone pBluescript (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Dll1 genomic DNA firefly (Photinus pyralis) cDNA
Depositor|Developer Kageyama, Ryuichiro |
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External Database
Mouse Dll1 firefly (Photinus pyralis)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)。
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          RDB14127 pDll1-Ub-Fluc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Shimojo, H., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Notch signaling pathway (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14127_A6Djp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB14127_A6Dja.seq check
          >D03343A1_A6Dj_2_M13_-40_E03_15.ab1
              1 CCTGCACAGG GATTGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG
             61 AGGTCGACGC GGCCGCTTGT AGTTCATTAT ATTTATTTTG GAAAAATATT TTTAAAATGA
            121 ATTTCTTCAT TAACAAAACA GTAAAAAACT CAAATAACAT TTGCACAATA TTCCATAAAT
            181 ACATTTATAT ACAAAAAAAT ATAGTCACAT AGACCCGAAG TGCCTTTGTA CATATTTACC
            241 AAAATTTAAA TTATAAAAAA ATGAACGTCA CAAAATACTC AAGCTTTACA GATATCATGA
            301 AAAATATTTT TACAAATCCC AAAAAAATAA CACACGTTTT CTTTTTCCCG TCTAGGAAAA
            361 AAAAAAACAC ATCTCAGTAT CAAAAAGGAC AATAAAGGCA GTTGTGTTTC TAGTTCAAGG
            421 AAAGACTGGC TCATAGTAAT CCAAGATAGA CGTGTGGGCA GTGCGTGCTT CCTATATGAA
            481 TCAAGTCACT CGTCCATTTA AATATATACT CTTCAAAGCA ACTGTCCATA GTGCAATGGG
            541 AACAACCAGC AGGCAGTCCA GCGGCAGCCA AAGCCTAGGC TGGCAGTGTC GGGCGCCTCT
            601 GCTAACTCTG AGAGAACCAG CTTCGCCTGA ACCTGGTTCT CAGCAGCAGT CCCTGGGTTT
            661 CCTCTCCCTT CCTCTCTCAG CAGCATTCAT CGGGGCTATA TCCTTGGAAT TTTATTTAAG
            721 AGAAATGGGA ATTTTGCCAC ATCGCTTCCA TCTTACACCT AGAAGGGGTC GACGGTATCG
            781 ATGTGAGGTT ACACGGCGAT CTTTCCGCCC TTCTTGGCCT TTATGAGGAT CTCTCTGATT
            841 TTTCTTGCGT CGAGTTTTCC GGTAAGACCT TTCGGTACTT CGTCCACAAA CACAACTCCT
            901 CCGCGCAACT TTTTTCGCGG TTGTTACTTT GACTGGCGAC GTAATCCACG ATCTCTTTTT
            961 TCCGTCATCG TCTTTCCGTG CTCCCAAAAC AACAACGGCG GCGGGAAAGT TTCACCGGCG
           1021 TCATCGTCGG AAGACTGCGA CACCTGCGTC GAAGATGTGG GGTGGTTGGA GCAGGATTGA
          1081 TCCGAATCAG CGGGAAGCCA CCTTGATAGC CTGTTTTGTT ACCTA
          //
          Primer: Reverse2
          Sequence file: RDB14127_A6Djb.seq check
          >D03343A1_A6Dj_2_Reverse2_H10_22.ab1
              1 ATACCGGGGA ATTACGGCCC AAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG
             61 GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTCCTG ATACCAGGTG AGGTGAGAGC
            121 ACTGTGGCCC GGGCCCAGCT GCATGGAACC AGCACGACTT CGTCTCAAAG CTTTGTGACT
            181 CAAGTCGTAC CCACATCCAC AGGCCCAGCG ATGGCCACCT CTCTAGCACC GCCACCGTCC
            241 AGAAGCCCCC AGGGGCGCTA ACTCTAGTTG AGAGTCAGAA ACGTATGAGC AAACTAAACG
            301 CAACCCCTCC CCTGTTAGAG CCTTCTTCCC ACAAACCCCT CACCCCATTG CTACCCATTC
            361 CAGACCAAAG GCCGCGGAAA GTTCCGAGCG AGGTGCACGC GTGACTCTCT TCGGCTGGCC
            421 ACGAGCAGCT GCTAGGATAA TCTGATCCTG GGACAAAGGC CCGCCCGCCC GGGCTGCAGC
            481 CGCAGGTAAA CACGCGCTTT GTTATGGAAA TCAGCGCAGG ACAGCCCGGA GCAGACAACC
            541 CACGATTCCG AGGAAGGAGA GGCATAGGGG GAGGGGCCTC GGGTGGGGCG ATCTGTCATC
            601 TGCGCGGCAG CCGCCAGCTG AGCCACGCGC CTCCCCTTTT CTCATGTAAA TCAGCACATG
            661 CCTTTGCAAC TTTCTGCAGC CGGCTTCCCA AGACCCACGC ACCCCAGGGT GAGGTGTCCT
            721 CGGAGCGCAT ACAATGGCTC TGAGTTCCTA TCTAGCTACC CAAAGGAAAA TCACCAACTA
            781 GGTGGGTAGA AGGAGGCGGC AGCCGGTTCC ATCAAAGAAG AAAGGGCTGT GATCCTACGG
            841 CATGACGGAG TTCGGACCCC GATCCACTCT GAAGTTCACG GGGCGCCATG CCGGAGTCTT
            901 TTGGGTTCAT TACTCCTTTT TGAGTGTACT GCCCACAGAA CGCAGAGGAG GCTAGGCCTA
            961 CCGGTTCCAC CTGGTGGGTG TGAATCTGAA GGCTCGAAGT CTCTAGTCTA ATTGGAAAGC
           1021 CAAATGCCTG AATTGCATTC AAGTGTGCAC ATGGTCTGTA CAACCATTAA GTGTGTGCGC
          1081 CAAGTATAAT GGGTTGGCAT GGCCTTTGTA A
          //
          Primer: Luc_R
          Sequence file: RDB14127_A6Djc.seq check
          >D03343A1_A6Dj_2_Luc_R_B01_04.ab1
              1 CATATGCTTC AAGGAACAGA GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG
             61 TTCCATCTTC CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCAA
            121 GCTTACTAGT CACCACACCT CTGAGACGGA GTACCAGGTG CAGGGTGGAC TCTTTCTGGA
            181 TGTTGTAGTC AGACAGGGTG CGCCCATCTT CCAGCTGTTT TCCAGCAAAG ATCAGCCTCT
            241 GCTGGTCAGG AGGGATGCCT TCCTTATCTT GGATCTTTGC CTTGACATTC TCAATGGTGT
            301 CACTGGGCTC AACCTCGAGG GTGATGGTCT TACCAGTCAG AGTCTTCACG AAGATCTGCA
            361 TGGTGGCGGC GGTTACCTTA ACATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG
            421 ATCCACTAGT CCGCTGGACG CCCTTGCTCT CCTAGAGTAT CCTCTTTTCT TTCTTCTTCA
            481 AAGACCCAGG GATGCCAGGA AAGTCTATAT GACCCGAGGA GTACTGGCGG AAAGGCGTCT
            541 CTCGGCTCCA GGTGTGCTAT CAGGGCCAGG TCGCTTCAGG AGGGTGCGGT GCCTTCAGCT
            601 AGGTCCCCGG CAAGAGCTCT ACGAGGCACT GAGAAAGGAC AACTTTCGGT TTCCTCTTCG
            661 TCTGGCTTTC AGTCCACGGA GAGGTGAGTG TCTCCTGCTT TCTGGTTTTG TCTTAAGCTT
            721 CTTCGCGGAG GGAAAGAGGA GAAAGGAGAT AAGACACAGA GGGAAGAGAG CCCAGATGTT
            781 CAGCTTAATT CCCAAGAGAG CTTGCAGTGG CTTCCTTCGC AGGCCTATTA GGAAGCGGCG
            841 GTCTGGAAAT CCCACCGGTG AGGCGATAAT CCGGGGCCCC GAGGGGCGCG GTGGGAACGG
            901 TGGTGGCAAG GACTGCGGCG CGCGCCCTGG TTTCCCGGCC CGGCGTCACT CGGCAGCCCG
            961 CTGCCGCTCC CAGAGTGTTC TAGACCGGGT ATCTCTTGCA CTATCGCCAG CCTCGCGTCT
           1021 TTCCCTCAAG AATTTCAGCC CAATGCCCAT GCGAGACACA CACCGAAGAA CCGGAGCCCG
          1081 AAAGCGTGGG AAGCTCGTTG CCGCACCGGG TGCTTCTTCC CCGGTGTAAT CT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shimojo, H., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl