Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV-GFP-ERK2

Expression vector of GFP-tagged human ERK2.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14156_A6Ecp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14156_A6Eca.seq
>D03441A3_A6Ec_2_CMV_Forward_C05_08.ab1     1 ACATCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG   121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT   181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT   241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC   301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA   361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA   421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG   481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA   541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA   601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA   661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG   721 CACCCAGTCC AAAGCTGAGC AAAGGACCCC AACGAGAAGC GCGATCACAT GGGTCCTGCT   781 GGAGTTCGTG ACCGCCGCCG GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGGC   841 CGGACTCAGA TCCAAGGCTT CGAATTCTGC AGTCGACGGT ACCCGCCGGG CCCATCAACA   901 AGTTTGTACA AAAAAGCAAG GGCTTTATCC CGGGCAGCCC CATATGGCGG CGGCGGGCCG   961 GCCGGGCGGG GCGCGGCCTC AGGAGAATGG TTCCCGCTGA GGTCAGTGTT CCGACGGTGA  1021 GAGCCGGCCG CCTACACCCA AAAACCCTCT TGTTACCATC CGGCCGAAGG GCAGTCAATA 1081 ACGGAGTTAC AAT //
Primer: EGFP-C Sequence file: RDB14156_A6Ecb.seq
>D03441A3_A6Ec_2_EGFP_C_D05_11.ab1     1 CGAAGGTTAT CTCGGGCATG GAGAGCTGTA CAGTCCGGCC GGACTCAGAT CCAAGCTTCG    61 AATTCTGCAG TCGACGGTAC CGCGGGCCCA TCAACAAGTT TGTACAAAAA AGCAGGCTTT   121 ATCCCGGCAG CCCATATGGC GGCGGCGGCG GCGGCGGGCG CGGGCCCGGA GATGGTCCGC   181 GGGCAGGTGT TCGACGTGGG GCCGCGCTAC ACCAACCTCT CGTACATCGG CGAGGGCGCC   241 TACGGCATGG TGTGCTCTGC TTATGATAAT GTCAACAAAG TTCGAGTAGC TATCAAGAAA   301 ATCAGCCCCT TTGAGCACCA GACCTACTGC CAGAGAACCC TGAGGGAGAT AAAAATCTTA   361 CTGCGCTTCA GACATGAGAA CATCATTGGA ATCAATGACA TTATTCGAGC ACCAACCATC   421 GAGCAAATGA AAGATGTATA TATAGTACAG GACCTCATGG AAACAGATCT TTACAAGCTC   481 TTGAAGACAC AACACCTCAG CAATGACCAT ATCTGCTATT TTCTCTACCA GATCCTCAGA   541 GGGTTAAAAT ATATCCATTC AGCTAACGTT CTGCACCGTG ACCTCAAGCC TTCCAACCTG   601 CTGCTCAACA CCACCTGTGA TCTCAAGATC TGTGACTTTG GCCTGGCCCG TGTTGCAGAT   661 CCAGACCATG ATCACACAGG GTTCCTGACA GAATATGTGG CCACACGTTG GTACAGGGCT   721 CCAGAAATTA TGTTGAATTC CAAGGGCTAC ACCAAGTCCA TTGATATTTG GTCTGTAGGC   781 TGCATTCTGG CAGAAATGCT TTCTAACAGG CCCATCTTTC CAGGGAAAGC ATTATCTTGA   841 CCAGCTGAAC CACATTTTGG GGTATTCTTG GGATCCCCCA TCACAAGAAA GACCTGAATT   901 GTATAAATAA ATTTTAAAAA GCTAGGAAAC TATTTTGCTT TTCTCTTTCC ACACAAAAAT   961 AAAGGTGCCC ATGGGAACAG GCTGTTCCCA AATGGCTGGA CTCCCAAGCT TCTGGACTTT  1021 ATTGGACAAA AAATGGTGGA CATTTCCAAT CCCACACTAG GAGGATGAGT TAGCAAACAG 1081 GCCTCTGGGC CCAAC //
Primer: ME_1290Rv Sequence file: RDB14156_A6Ecc.seq
>D03441A3_A6Ec_2_ME_1290RV_E05_14.ab1     1 ATGGCCAATT CATTTTATGG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA    61 AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT   121 CCCATCAACC ACTTTGTACA AGAAAGCTGG GTCTAGATAT CTCGAGAACT AGTGATCCTC   181 TGAGCCCTTG TCCTGACAAA TTTAAGATCT GTATCCTGGC TGGAATCTAG CAGTCTCTTC   241 AAAAATTAGT TCTTTGAGCT TTTCCTTAGG CAAGTCATCC AATTCCATGT CGAACTTGAA   301 TGGTGCTTCG GCGATGGGCT CGTCACTCGG GTCGTAATAC TGCTCCAGAT ATGGGTGGGC   361 CAGAGCCTGT TCTACTTCAA TCCTCTTGTG TGGGTTGAAT GTCAACATTT TGTCCAATAA   421 GTCCAGAGCT TTGGAGTCAG CATTTGGGAA CAGCCTGTTC CATGGCACCT TATTTTTGTG   481 TGGAAGAGAA AGCAAATAGT TCCTAGCTTT TAAATTTATT ATACAATTCA GGTCTTCTTG   541 TGATGGGGAT CCAAGAATAC CCAAAATGTG GTTCAGCTGG TCAAGATAAT GCTTCCCTGG   601 AAAGATGGGC CTGTTAGAAA GCATTTCTGC CAGAATGCAG CCTACAGACC AAATATCAAT   661 GGACTTGGTG TAGCCCTTGG AATTCAACAT AATTTCTGGA GCCCTGTACC AACGTGTGGC   721 CACATATTCT GTCAGGAACC CTGTGTGATC ATGGTCTGGA TCTGCAACAC GGGCCAGGCC   781 AAAGTCACAG ATCTTGAGAT CACAGGTGGT GTTGAGCAGC AGGTTGGAAG GCTTGAGGGT   841 CACGGTGCAG AACGTTAGCT GAATGGATAT ATTTTTAACC CCTCTGAGGA TCTGGTAGAG   901 AAAATAGCAG ATATGGGTCA TTGGCTGAAG GTGGTTGTGT CTTCAAGAGC TTTGTAAAGA   961 TCTGTTTCCA TGAGGTCCCT GTACTATATA TACATCTTTC ATTGCTCGAT GGTTTGGGTG  1021 CTCGGAATAA TGTCATTGGA TTTCCATGGA ATGGTTCTCA AGTATCTGAG GCGCCAGTTA //
Primer: SV40pro_F_V2 Sequence file: RDB14156_A6Ecd.seq
>D03441A3_A6Ec_2_SV40pro_F_V2_F05_17.ab1     1 GTGGGCGTTT TTTGGAGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG    61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG   901 CCCCAACCTG CCATCACGAG ATTTCGATTC CACCGGCCGC CCTTCTATGA AAGTTTGGGG   961 CTTCGGAATC GTTTTTCCCG GGGACGGCCG GCTTGGAATG GATCCCTTCC AAGCGGCGGG 1021 GAATCCTCAT GGCTTGAAGA TCATTCGCCT ATCCCTTAGG GGGGATGGCC TAACC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl