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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human MLC20 T18A

Shuttle vector to generate recombinant adenovirus expressing human MYL12B (mutant, T18A).

Catalog number RDB14159
Resource name human MLC20 T18A
Clone info. Shuttle vector to generate recombinant adenovirus expressing human MYL12B (mutant, T18A).
Comment Expression was confirmed by the depositor with immunoblot and microphotographs.
Vector backbone pAxCAwtit (Cosmid, use packaging extracts for transformation)
Size of vector backbone 45 kb
Selectable markers Amp^r
Gene/insert name Human MYL12B cDNA
Depositor|Developer Hirano, Katsuya |
Other clones in our bank
External Database
Human MYL12B
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, M. et al., Sci. Rep. 6:20989, 2016)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, M. et al., Sci. Rep. 6:20989, 2016)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14159 human MLC20 T18A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hirano, M., Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci. Rep. 6: 20989 (2016). PMID 26863988.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14159_A6Epp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB14159_A6Epa.seq check
          >D03455A2_A6Ep_pAxCA_F1_A12_03.ab1
              1 GACCGGGGCA ACCATGTTCA GCCTTCTTCT TTTTCCTAAC AGCTCCTGGG CAACGTGCTG
             61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGATTT ATCGATTTGA TCCCCACCAT
            121 GTCGAGCAAA AAGGCAAAGA CCAAGACCAC CAAGAAGCGC CCTCAGCGTG CAGCATCCAA
            181 TGTGTTTGCC ATGTTTGACC AGTCACAGAT TCAGGAGTTC AAAGAGGCCT TCAACATGAT
            241 TGATCAGAAC AGAGATGGCT TCATCGACAA GGAAGATTTG CATGATATGC TTGCTTCTCT
            301 AGGGAAGAAT CCCACTGATG CATACCTTGA TGCCATGATG AATGAGGCCC CAGGGCCCAT
            361 CAATTTCACC ATGTTCCTGA CCATGTTTGG TGAGAAGTTA AATGGCACAG ATCCTGAAGA
            421 TGTCATCAGA AACGCCTTTG CTTGCTTTGA TGAAGAAGCA ACAGGCACCA TTCAGGAAGA
            481 TTACCTAAGA GAGCTGCTGA CAACCATGGG GGATCGGTTT ACAGATGAGG AAGTGGATGA
            541 GCTGTACAGA GAAGCACCTA TTGACAAAAA GGGGAATTTC AATTACATCG AAGTTCACAC
            601 GCATCCTGAA ACATGGAGCC AAAGACAAAG ATGACTGAAA AGAACTTTAG CTAAAAGGGC
            661 CCGCGAAATT AATTAAAACT AGTCTAGAAA TCGATAATCA ATTCACTCCT CAGGTGCAAG
            721 GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCCAATGCC CCTGGCTCAC AAAATACCAC
            781 TGAGATCTTT TTTCCCTCTG CCAAAAAATT ATGGGGGACA TCATGAAAGC CCCCTTTGAG
            841 CATCTGACTT TCTGGCTAAT AAAAGGAAAA TTTATTTTTT CATTGGCAAT AGTGTGTTGG
            901 GAATTTTTTT GTGTCCTCTC ACCTCGGAAG GGACATATGG GGAAGGGCAA ATCAATTTAA
            961 AAACATCAGA ATGAGTATTT TGGTTTAGAG GTTTGGCCAA CATTATGCCC CAAATGCTTG
           1021 GCCTGCCATG AACAAAAGCT TGGCTATTAA AAGAGAGTCA ATCAGATATA TATGAAACAA
           1081 CCCCCTCATG CTGGTCCACT CCGTATTTCC ATAGAAAAGC CCTGGATAAG AAAGTTTTTT
          1141 ATTTTGTATG TAATTTCTAA TT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Hirano, M., Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci. Rep. 6: 20989 (2016). PMID 26863988.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl